Reduced Neovascularization in Aged Rats: A Study Using Lipopolysaccharide-induced Inflammation

Authors

  • Maethinee Sakhakorn
  • Sheepsumon Viboolvorakul
  • Nipan Israsena
  • Suthiluk Patumraj

Keywords:

aging, neovascularization, lipopolysaccharide, oxidative stress

Abstract

Aged tissues are associated with reduced capillary density and delayed neovascularization. The aging environment is characterized by increased oxidative stress, which damages macromolecules, and thereby causes apoptosis of endothelial cells. Lipopolysaccharide (LPS) has been shown to have angiogenic property. Therefore, the objective of this study was to examine whether neovascularization after LPS-induced inflammation in aged rats is decreased when compared to adult rats. Male Wistar rats were divided into four groups: adult (aged 6-8 months), aged (aged 22-24 months), adult + LPS, and aged + LPS. Blood perfusion, capillary vascularity, plasma and tissue MDA, and tissue VEGF levels were measured. Plasma MDA in aged group was higher than in adult group (P < 0.05). Blood perfusion and capillary vascularity (%CV) in aged group were lower than in adult group (P < 0.05). Blood perfusion and %CV in aged + LPS group were significantly lower than those in adult + LPS group. Aged rats have reduced capillary vascularity when compared to adult rats. LPS could induce neovascularization in both adult and aged rats. However, aged rats have reduced neovascularization after LPS pre-treatment when compared to adult + LPS rats.

Author Biographies

Maethinee Sakhakorn

Inter-department of Physiology, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand.

Sheepsumon Viboolvorakul

Physiology Unit, Department of Medical Science, Faculty of Science, Rangsit University, Pathum Thani 12000, Thailand.

Nipan Israsena

Stem Cell and Cell Therapy Research Unit, Department of Pharmacology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

Suthiluk Patumraj

Center of Excellence for Microcirculation, Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

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Published

2013-09-30

How to Cite

1.
Sakhakorn M, Viboolvorakul S, Israsena N, Patumraj S. Reduced Neovascularization in Aged Rats: A Study Using Lipopolysaccharide-induced Inflammation. J Physiol Biomed Sci [Internet]. 2013 Sep. 30 [cited 2024 Dec. 14];26(1):9-12. Available from: https://li01.tci-thaijo.org/index.php/j-pbs/article/view/250961

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Original Articles