A Study of the Effect of Methotrexate and Vitamin A on NOR Expression in Hepatocytes of Male Wistar Rats

Authors

  • Rao Yeshwanth
  • Laxminarayana K Bairy
  • Regunathan Deepthinath
  • Bhat Ramachandra

Abstract

The effects of methotrexate (MTX) and vitamin Ahich resulted in the reduction in the NOR count. on NOR expression and histopathology in liver of male Wistar rats were evaluated. Male Wistar rats aged 4 months and maintained in our institution, were used in the present study. Animals were injected intraperitoneally with 3 different doses of methotrexate in alternative days for one week and the animals were sacrificed on the seventh day by using ether. Paraffin sections of the liver were prepared and histopathology as well as AgNOR studies were done. The same procedure was repeated with vitamin A and with both vitamin A and methotrexate. MTX treatment showed disruption of normal hepatic plates, vaculation of the hepatocytes, nuclear variability and enlarged blood vessels. When MTX was given with vitamin A, the latter reduced the damage done by MTX. In AgNOR study, data showed an overall decrease in the total NOR count (numerical) from normal to 12 mg dose MTX. The total count includes small, medium and large sized NORs. A statistically high significant difference was found from normal to 12 mg dose MTX as well as between the doses. The genotoxicity in rats is well documented with high dose of methotrexate (12 mg/kg) compared to low dose (8 mg/kg). Methotrexate treatment reduced the transcription rate and ploidy. Methotrexate reduced the cell proliferation w

Author Biographies

Rao Yeshwanth

Department of Physiology, Melaka Manipal Medical College (Manipal Campus) Karnataka 576104, India.

Laxminarayana K Bairy

Department of Pharmacology, Kasturba Medical College Manipal, Karnataka 576104, India.

Regunathan Deepthinath

Department of Anatomy, Melaka Manipal Medical College (Manipal Campus) Karnataka 576104, India.

Bhat Ramachandra

Department of Anatomy, Kasturba Medical College Manipal, Karnataka 576104, India.

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Published

2010-09-23

How to Cite

1.
Yeshwanth R, Bairy LK, Deepthinath R, Ramachandra B. A Study of the Effect of Methotrexate and Vitamin A on NOR Expression in Hepatocytes of Male Wistar Rats. J Physiol Biomed Sci [Internet]. 2010 Sep. 23 [cited 2024 Dec. 10];23(1):1-7. Available from: https://li01.tci-thaijo.org/index.php/j-pbs/article/view/251043

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Original Articles