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Respiratory disease conditions are causing heavy economic losses in the poultry industry. Ornithobacteriosis is one of the infectious diseases of avian species that has been observed in the poultry flocks of many different countries and is continuing to emerge as a growing concern in the economic viability of the poultry industry. The objectives of this study were to detect and identify the ORT from layer flocks in Chiang Mai, Thailand. Layer antibodies were determined in the serum samples of 30 clinically infected layer flocks of different ages (18-63 weeks) by ELISA. The serum analysis showed that the antibody responses were 78% negative, 7% suspect, and 15% positive. Older layers (41-63 weeks) revealed higher positive results than the younger aged layers (18-38 weeks). The bacteria were isolated and identified by polymerase chain reaction (PCR). Bacterial isolation and identification revealed that out of the eleven isolates tested, 8 showed positive results to PCR analysis. This study is very useful and is the first report about ORT found in layer flocks in Chiang Mai where the ORT vaccines are not available. Most importantly, this research has found that both bacteriological and molecular techniques can be used to detect ORT.
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