Isolation of Lactic Acid Bacteria from Mantis Shrimp (Harpiosquilla raphidea) Intestine for Probiotic Development.

Abstract

The collecting sample of mantis shrimp from traditional fishing area of Thasala, Nakhon Si Thammarat province were counted for total bacteria and isolated for lactic acid bacteria (LAB) from mantis shrimp intestinal. It was found that 139 isolates of LAB could be isolated.  Probiotic properties showed that 7 isolates could survive at low pH (pH 3-6), tolerated to bile salt (0.15% and 0.3%), utilized starch, protein and lipid, and could grow in anaerobic condition. Moreover, it could inhibite Vibrio parahaemolyticus and V. harveyi. For the studies of growth and inhibition properties, 7 isolates LAB showed the highest growth rate at 33 hours in MRS broth (pH 6.0) incubated at 35^oC. The results revealed that isolated LAB from mantis shrimp intestine had probiotic properties. Therefore, it is possible that the isolated LAB can be developped into probiotics.