Screening of Lactic Acid Bacteria Producing Bacteriocin Capable to Inhibit the Growth of Bovine Mastitis Pathogens
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Abstract
Bovine mastitis treatment with antibiotics may cause an impact on the quality of raw milk due to the occurrence of milk residues. Bacteriocin is a peptide or protein synthesized by bacteria. It can be used as a safe food preservative and used for mastitis treatment. This research aimed to isolate bacteriocin-producing lactic acid bacteria from pickled cockle, sour pork (Nham), pig and cow dungs using agar spot test. Antibacterial capacity against mastitis pathogens: Streptococcus agalactiae and Streptococcus uberis were determined. It was found that 57 isolated lactic acid bacteria were gram positive, rod shaped, non-spore forming, non-motile, gas-producing or non gas-producing, and catalase negative. There were 36 and 32 isolated bacteria that could inhibit S. agalactiae and S. uberis, respectively. The inhibitory activities of bacterial isolates LAB 21, LAB 31, and LAB 34 were decreased or disappeared when tested their Cell-free neutralized supernatant (CFNS) with protease. The results revealed that CFNS were peptide. The efficacy of bacteriocins tested by agar well diffusion showed that bacterial isolates LAB 21, LAB 31 and LAB 34 had clear zones of 14.50, 12.50 and 14.00 mm against S. agalactiae respectively, whereas they had lower clear zones of 10.75, 10.50 and 11.50 mm against S. uberis, respectively. There were no significant differences between clear zones of LAB 21, LAB 34 and amoxicillin on S. agalactiae at 95% confidence interval (p≤ 0.05).
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