Suitable Carbon Sources and C/N Ratio for Biofloc Production
Main Article Content
Abstract
This study aims to investigate the appropriate carbon sources and C/N ratio required for biofloc formation. This study involves two experiments. The first experiment compares the use of three different carbon sources i.e. rice flour, rice bran and molasses for biofloc formation. The results showed that total ammonia of the treatment groups that added rice flour, rice bran, and molasses was statistically lower than that of the control group (p<0.05). The treatment group that added rice bran could reduce total ammonia most effectively. Besides, total suspended solids in the water of the treatment group with added rice bran were statistically higher than those of the treatment groups with added rice flour and molasses and those of the control group (p<0.05). Therefore, in the second experiment, rice bran was chosen as a potential carbon source for biofloc formation while urea was selected as a source of nitrogen to find out a suitable carbon-nitrogen (C/N) ratio for biofloc formation. The effect of four different initial C/N ratios (10:1, 15:1, 20:1 and no carbon sources) were evaluated. The results indicated that a C/N ratio of 20:1 could reduce total ammonia most effectively (91.62%) and total suspended solids in water were statistically higher (p<0.05) than using the other ratios. In conclusion, the most suitable carbon source was rice bran and the appropriate C/N ratio for biofloc formation was 20:1 as they could reduce total ammonia most effectively.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The content and information in the article published in Journal of Rajamangala University of Technology Srivijaya It is the opinion and responsibility of the author of the article. The editorial journals do not need to agree. Or share any responsibility.
References
Anand, P.S., Kohli, M.P.S., Kumar, S., Sundaray, J.K., Roy, S., Venkateshwarlu, G., Sinha, A. and Pailan, G.H. 2013. Effect of dietary supplementation of biofloc on growth performance and digestive enzyme activities in Penaeus monodon. Aquaculture 418-419: 108-115.
APHA. 1980. Standard methods for the examination of water and wastewater. American public health association, Washington DC.
Asaduzzaman, M., Wahab, M.A., Verdegem, M.C.J., Huque, S., Salam, M.A. and Azim, M.E. 2008. C/N ratio control and substrate addition for periphyton development jointly enhance freshwater prawn Macrobrachium rosenbergii production in ponds. Aquaculture 280: 117
-123.
Asaduzzaman, M., Wahab, M.A., Verdegem, M.C.J., Adhikary, R.K., Rahman, S.M.S., Azim, M.E. and Verreth, J.A.J. 2010. Effects of carbohydrate source for maintaining a high C:N ratio and fish driven re-suspension on pondecology and production in periphyton-based freshwater prawn culture systems. Aquaculture 37: 46-301.
Avnimelech, Y., Mozes, N., Shaher Diab, S. and Kochba, M. 1995. Rates of organic carbon and nitrogen degradation in intensive fish ponds. Aquaculture 134: 211-216.
Avnimelech, Y. 1999. C/N ratio as a control element in aquaculture systems. Aquaculture 176(3-4): 227-235.
Avnimelech, Y. 2009. Biofloc Technology-A Practical Guide Book. 1sted. World Aquaculture Society, Baton Rouge, LA.
Azim, M.E. and Little, D.C. 2008. The biofloc technology (BFT) in indoor tanks: Water quality, biofloc composition, and growth and welfare of Nile tilapia (Oreochromis niloticus). Aquaculture 283: 29-35.
Boyd, C.E. and Fast, A.W. 1992. Pond monitoring and management, pp. 497-513. In Fast, A.W. and Lester, L.J., eds. Marine shrimp culture: principles and practices. Developments in aquaculture and fisheries science, volume 23. 1sted. Elsevier Science Publisher B.V., Amsterdam.
Boyd, C.E. and Tucker, C.S. 1992. Water quality and pond soil analyses for aquaculture. 1sted. Alabama Agricultural Experiment Station, Auburn University, Alabama.
Boyd, C.E. and Tucker, C.S. 1998. Pond aquaculture water quality management. 1sted. Kluwer academic publishers, New York.
Burford, M.A., Preston, N.P., Glibert, P.M. and Dennison, W.C. 2004. Tracing the fate of 15N enriched feed in an intensive shrimp system. Aquaculture 206: 199-216.
Cao, L., Wang, W., Yang, Y., Yang, C., Yuan, Z., Xiong, S. and Diana, J. 2007. Environmental impact of aquaculture and countermeasures to aquaculture pollution in China. Environment Science Pollution Research 14(7): 452-462.
Crab, R., Avnimelech, Y., Defoirdt, T., Bossier, P. and Verstraete, W. 2007. Nitrogen removal techniques in aquaculture for a sustainable production. Aquaculture 270: 1-14.
Crab, R., Kochva, M., Verstraete, W. and Avnimelech, Y. 2009. Bio-flocs technology application in over-wintering of tilapia. Aquaculture Engineering 40: 105-112.
Crab, R., Defoirdt, T., Bossier, P. and Verstraete, W. 2012. Biofloc technology in aquaculture: beneficial effects and future challenges. Aquaculture 356: 351-356.
Forteath, N. 1990. A handbook on recirculating systems for aquatic organisms. 1sted. Fishing industry training board of Tasmania Inc, Hobart.
Hargreaves, J.A. 1998. Nitrogen biogeochemistry of aquaculture ponds. Aquaculture 166: 181-212.
Hargreaves, J.A. 2013. Biofloc production systems for aquaculture. SRAC publication, United States.
Hari, B., Kurup, M.B., Varghese, T., Schrama, J.W. and Verdegem, M.C.S. 2004. Effects of carbohydrate addition on production in extensive shrimp culture systems. Aquaculture 241: 179-194.
Koydon, S. 2014. Nitrogen elimination in zero waste aquaculture system. RMUTSB Academic Journal 2(1): 66-80. (in Thai)
Naylor, R.L., Goldburg, R.J., Primavera, J.H., Kautsky, N., Beveridge, M.C.M., Clay, J., Folke, C., Lubchenco, J., Mooney, H. and Troell, M. 2000. Effect of aquaculture on world fish supplies. Nature 405: 1017-1024.
Roselien, C., Tom, D., Peter, B. and Willy, V. 2012. Biofloc technology in aquaculture: Beneficial effect and future challenges. Aquaculture 356-357: 351-356.
Serra, P.T., Gaona, A.C., Furtado, S.P., Poersch, H.L. and Wasielesky, W. 2015. Use of different carbon sources for the biofloc system adopted during the nursery and grow-out culture of Litopenaeus vannamei. Aquaculture International 23(6): 1-16.
Van Wyk, P. and Scarpa, J. 1999. Water quality and management, pp 128-138. In Van Wyk, P., Hodgkins, M.D., Laramore, R., Main, K.L., Mountain, J. and Scarpa, J., eds. Farming marine shrimp in recirculating freshwater systems. Harbor Branch Oceanographic Institution, Tallahassee.
Wankanapol, A., Chaibu, P. and Soonthornvipat, S. 2017. Evaluation of different carbon sources for biofloc production in tilapia (Oreochromis niloticus L.) culture. Silpakorn University Sciences & Technology Journal 11(3): 17-24.
Wasielesky, W., Atwood, H., Stokes, A. and Browdy, C.L. 2006. Effect of natural production in a zero-exchange suspended microbial floc based super-intensive culture system for white shrimp Litopenaeus vannamei. Aquaculture 258: 396-403.