Germination, Vigor and Seedling Growth of Soybean after Seed Coating and Pelleting with Bacillus subtilis

Main Article Content

Jakkrapong Kangsopa
Phetcarat Jeephet
Sureemard Chantain

Abstract

Soybeans are an important economic crop in Thailand. However, the soybean seeds used for growing soybeans lack quality and standards. This results in inconsistent and weak seedlings after cultivation, which can be easily destroyed by diseases and pesticides. This current experiment aims to study the effect of seed coating and seed pelleting using Bacillus subtilis on the germination, vigor, and growth of soybean seedlings. The experiment was conducted at the Seed Technology Laboratory, Agronomy Major, Faculty of Agricultural Production, Maejo University. An experimental design was done in a completely randomized design (CRD). The results of the experiment were as follows. Seeds coated with 0.5 milliliters of B. subtilis had the highest rate of radicle emergence at 99 %. Moreover, the speed of radicle emergence and speed of germination were statistically significantly higher compared to untreated seeds. On the other hand, all seeds coated with the B. subtilis and normal coated treatments gave the best germination rate. In addition, seeds coated with 0.5 milliliters of B. subtilis still had higher shoot length, root length, and total seedling quality when compared with untreated seeds under laboratory conditions. Under greenhouse conditions, compared to seed coating and pelleting, seed coating in combination with B. subtilis gave the best germination. Therefore, seed coating with 0.5 milliliters of B. subtilis is the most suitable method and recommended rate for soybean seed enhancement.

Article Details

How to Cite
Kangsopa, J., Jeephet , P., & Chantain, S. (2022). Germination, Vigor and Seedling Growth of Soybean after Seed Coating and Pelleting with Bacillus subtilis. Rajamangala University of Technology Srivijaya Research Journal, 14(1), 266–281. Retrieved from https://li01.tci-thaijo.org/index.php/rmutsvrj/article/view/245464
Section
Research Article
Author Biographies

Jakkrapong Kangsopa, Division of Agronomy, Faculty of Agricultural Production, Maejo University.

Division of Agronomy, Faculty of Agricultural Production, Maejo University, San Sai, Chiang Mai 50290, Thailand.

Phetcarat Jeephet , Division of Agronomy, Faculty of Agricultural Production, Maejo University.

Division of Agronomy, Faculty of Agricultural Production, Maejo University, San Sai, Chiang Mai 50290, Thailand.

Sureemard Chantain, Division of Agronomy, Faculty of Agricultural Production, Maejo University.

Division of Agronomy, Faculty of Agricultural Production, Maejo University, San Sai, Chiang Mai 50290, Thailand.

References

Accinelli, C., Abbas, H.K., Little, N.S., Kotowicz, J.K. and Shier, W.T. 2018. Biological control of aflatoxin production in corn using non-aflatoxigenic Aspergillus flavus administered as a bioplastic-based seed coating. Crop Protection 107(2018): 87-92.

Arshad, M., Saleem, M. and Hussain, S. 2007. Perspectives of bacterial ACC deaminase in phytoremediation. Trends in Biotechnology 25(8): 356-362.

Ashraf, M. and Foolad, M.R. 2005. Pre-sowing seed treatment-a shotgun approach to improve germination, plant growth, and crop yield under saline and non-saline conditions. Advances in Agronomy 88(2005): 223-271.

Baki, A. and Anderson, J.D. 1973. Vigor determination in soybean seed by multiple criteria. Crop Science 13(6): 630- 633.

Cakmakci, R., Erat, M., Erdogan, U. and Donmez, M.F. 2007. The influence of plant growth-promoting rhizobacteria on growth and enzyme activities in wheat and spinach plants. Journal of Plant Nutrition and Soil Science 170(2): 288-295.

Chanprasert, W. 2010. Seed Physiology. Department of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok. (in Thai)

Chindaprasirt, P., Boonserm, K., Chairuangsri, T., Vichit-Vadakan, W., Eaimsin, T., Sato, T. and Pimraksa, K. 2011. Plaster material from waste calcium sulfate containing chemicals, organic fibers and inorganic additive. Construction and Building Materials 25: 3193-3203.

Department of Internal Trad. 2020. Situation of the Soybean Seed. Available Source: https://bit.ly/3pOcLkU, October 28, 2021. (in Thai)

Glick, B.R. 2012. Plant growth-promoting bacteria: mechanisms and applications. Scientifica 2012(5): 1-15. Article ID 963401.

Glick, B.R., Cheng, Z., Czarny, J. and Duan, J. 2007. Promotion of plant growth by ACC deaminase-producing soil bacteria. European Journal of Plant Pathology 119(3): 329-339.

ISTA. 2019. International Rules for Seed Testing, Edition 2019. International Seed Testing Association, Bassersdorf.

Junges, E., Toebe, M., Feliciano dos Santos, R., Finger, G. and Muniz, M.F.B. 2013. Effect of priming and seed-coating when associated with Bacillus subtilis in maize seeds. Revista Ciência Agronômica 44(3): 520-526.

Kangsopa, J. 2019. Seed Coating. Journal of Agricultural Production 1(2): 63-76. (in Thai)

Kangsopa, J. 2020. Binder material for seed pelleting. Khon Kaen Agriculture Journal 48(1): 119-130. (in Thai).

Keereetaweep, R., Kaewmeechai, S., Chotiyarnwong, A., Gong-in, S., Phothaen, N. and Kajonmalee, V. 1987. Soybean varietal improvement for rust resistance, pp. 49-58. In Seminar Report: the 2nd Soybean Research. Pitsanulok. (in Thai)

Kesan, J.P. 2007. Agricultural Biotechnology and Intellectual Property: Seeds of Change. CAB International, Wallingford, UK.

Khorasani, A.C. and Shojaosadati, S.A. 2017. Starch- and carboxymethylcellulose-coated bacterial nanocellulose-pectin bionanocomposite as novel protective prebiotic matrices. Food Hydrocolloids 63(2017): 273-285.

Kloepper, J.W., Ryu, C.M. and Zhang, S. 2004. Induced systemic resistance and promotion of plant growth by Bacillus spp. The American Phytopathological Society 94(11): 1259-1266.

Kundu, B.S. and Gaur, A.C. 1980. Establishment of nitrogen-fixing and phosphate-solubilizing bacteria in rhizosphere and their effect on yield and nutrient uptake of wheat crop. Plant and Soil 57(1980): 223-230.

Lakshminarayana, K., Narula, N., Hooda, I.S. and Faroda, A.S. 1992. Nitrogen economy in wheat (Triticum aestivum) through use of Azotobacter chroococcum. Indian Journal of Agricultural Sciences 62(1): 75-76.

Martínez, L.L., Peniche, R.M., Iturriaga, M.H. and Arvizu-Medrano, S.M. 2013. Characterization of rhizobacteria isolated from tomato and their effect on tomato and bell pepper growth. Revista Fitotecnia Mexicana 36(1): 63-69.

Oteino, N., Lally, R.D., Kiwanuka, S., Lloyd, A., Ryan, D., Germaine, K.J. and Dowling, D.N. 2015. Plant growth promotion induced by phosphate solubilizing endophytic Pseudomonas isolates. Frontier in Microbiology 6(2015): 745.

Pa-oblek, S. 2015. Research and Development on Soybean. Department of Agricultural, Bangkok. (in Thai)

Pedrini, S., Merritt, D.J., Stevens, J. and Dixon, K. 2017. Seed Coating: Science or Marketing Spin?. Trends in Plant Science 22(2): 106-116.

Priestley, D.A. 1986. Seed Aging: Implications for Seed Storage and Persistence in the Soil. Comstock Publishing Associates, London.

Punsukumtana, L. 2009. Reuse Plaster. Ceramics Journal 3(3): 34-35. (in Thai)

Ramyabharathi, S., Meena, B. and Raguchander, T. 2013. Induction of defense enzymes and proteins in tomato plants by Bacillus subtilis EPCO16 against Fusarium oxysporum f. sp. Lycopersici. Madras Agricultural Journal 100(Special Issue): 126-130.

Rekha, P.D., Lai, W., Arun, A.B. and Young, C.C. 2007. Effect of free and encapsulated Pseudomonas putida CC-FR2-4 and Bacillus subtilis CC-pg104 on plant growth under gnotobiotic conditions. Bioresource Technology 98(2): 447-451.

Rocha, I., Ma, Y., Carvalho, M.F., Magalhães, C., Janoušková, M. and Vosátka, M. 2019. Seed coating with inocula of arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria for nutritional enhancement of maize under different fertilization regimes. Archives of Agronomy and Soil Science 65(1): 31-43.

Rowe, R.C., Sheskey, P.J. and Quinn, M.E. 2009. Handbook of Pharmaceutical Excipients. 6th ed. Pharmaceutical Press, London.

Siri, B. 2015. Seed Conditioning and Seed Enhancements. Klungnanawitthaya Priting, Khon Kaen. (in Thai).

Taylor, A.G., Allen, P.S., Bennett, M.A., Bradford, K.J., Burris, J.S. and Misra, M.K. 1998. Seed enhancements. Seed Science Research 8(2): 245-256.

Thomma, K. and Sirithorn, P. 2012. Effect of antagonistic bacterium Bacillus subtilis B006 as seed coating for control of Botryosphaeria rhodina, cause of gummosis disease. Khon Kaen Agriculture Journal 40(1): 53-60. (in Thai)

Tu, L., He, Y.H., Shan, C.H. and Wu, Z.S. 2016. Preparation of microencapsulated Bacillus subtilis SL-13 seed coating agents and their effects on the growth of cotton seedlings. BioMed Research International 2016: 1-7. DOI: 10.1155/2016/3251357

Widnyana, K.I. and Javandira, C. 2016. Activities Pseudomonas spp. and Bacillus sp. to stimulate germination and seedling growth of tomato plants. Agriculture and Agricultural Science Procedia 9(2016): 419-423.

Yang, O., Li, C., Li, H., Li, Y. and Yu, N. 2009. Degradation of synthetic reactive azo dyes and treatment of textile wastewater by a fungi consortium reactor. Biochemical Engineering Journal 43(3): 225-230.

Zaidi, S., Usmani, S., Singh, B.R. and Musarrat, J. 2006. Significance of Bacillus subtilis strain SJ 101 as a bioinoculant for concurrent plant growth promotion and nickel accumulation in Brassica juncea. Chemosphere 64(6): 991-997.