Effect of Sodium Hypochlorite and Cytokinin Concentrations on Survival and Shoot Development of In Vitro Damask Rose (Rosa damascena Mill.)
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Abstract
In the present, environmental variation is an important problem for propagation of damask rose grown under field condition which affects survival, quantity and quality of damask rose. Therefore, the objective of this study was to investigate the effect of sodium hypochlorite and cytokinin concentrations on micropropagation of in vitro Damask rose. The experimental design was completely randomized design with 5 replications. The results showed that the highest contamination of 40.00±0.00% was obtained after single node explants of damask roses were sterilized by 5% and 10% (v/v) sodium hypochlorite and cultured on the Murashige and Skoog (1962) for 28 days. In contrast, the highest survival and development of 76.67±1.67% and 53.33±3.33%, respectively occurred when single node explants were sterilized by 15% (v/v) sodium hypochlorite. The greatest of shoot number (2.67±0.17) and leaf number (35.00±0.50) were found on MS medium supplemented with 2.00 mg L-1 BAP. In addition, the highest shoot length (2.05±0.15 cm), root number (2.17±1.01) and root length (2.13±0.82 cm) were achieved when shoot explants were cultured on the MS medium without BAP for 42 days.
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