Effect of Sodium Hypochlorite and Cytokinin Concentrations on Survival and Shoot Development of In Vitro Damask Rose (Rosa damascena Mill.)

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Thanapoom Siringam
Wasinee Pongprayoon


In the present, environmental variation is an important problem for propagation of damask rose grown under field condition which affects survival, quantity and quality of damask rose. Therefore, the objective of this study was to investigate the effect of sodium hypochlorite and cytokinin concentrations on micropropagation of in vitro Damask rose. The experimental design was completely randomized design with 5 replications. The results showed that the highest contamination of 40.00±0.00% was obtained after single node explants of damask roses were sterilized by 5% and 10% (v/v) sodium hypochlorite and cultured on the Murashige and Skoog (1962) for 28 days. In contrast, the highest survival and development of 76.67±1.67% and 53.33±3.33%, respectively occurred when single node explants were sterilized by 15% (v/v) sodium hypochlorite. The greatest of shoot number (2.67±0.17) and leaf number (35.00±0.50) were found on MS medium supplemented with 2.00 mg L-1 BAP. In addition, the highest shoot length (2.05±0.15 cm), root number (2.17±1.01) and root length (2.13±0.82 cm) were achieved when shoot explants were cultured on the MS medium without BAP for 42 days.


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Siringam, T., & Pongprayoon, W. (2022). Effect of Sodium Hypochlorite and Cytokinin Concentrations on Survival and Shoot Development of In Vitro Damask Rose (Rosa damascena Mill.). Rajamangala University of Technology Srivijaya Research Journal, 14(2), 296–308. Retrieved from https://li01.tci-thaijo.org/index.php/rmutsvrj/article/view/246853
Research Article
Author Biographies

Thanapoom Siringam, Modern Agricultural Technology Management Program, Faculty of Science and Technology, Phranakhon Rajabhat University

Modern Agricultural Technology Management Program, Faculty of Science and Technology, Phranakhon Rajabhat University, Anusawaree, Bangkhen, Bangkok 10220, Thailand.

Wasinee Pongprayoon, Department of Biology, Faculty of Science, Burapha University.

Department of Biology, Faculty of Science, Burapha University, Saensook, Muang, Chonburi 20131, Thailand.


Abbaszadeh, M., Kazerani, H.R. and Kamrani, A. 2010. Laxative effects of Rosa damascena Mill. in dogs. Journal of Applied Animal Research 38(1): 89-92.

Aridogan, B.C., Baydar, H., Kaya, S., Demirci, M., Ozbasar, D. and Mumcu, E. 2002. Antimicrobial activity and chemical composition of some essential oils. Archives of Pharmacal Research 25(6): 860-864.

Autaijamsripon, J., Jirakiattikul, Y., Rithichai, P. and Itharat, A. 2019. Effect of plant growth regulators on in vitro shoot multiplication and root induction of Ocimum sanctum L. (Holy Basil Purple Type). Thai Science and Technology Journal 27(4): 630-638. (in Thai)

Baig, M.M.Q., Hafiz, I.A., Hussain, A., Ahmad, T. and Abbasi, N.A. 2011. An efficient protocol for in vitro propagation of Rosa gruss an teplitz and Rosa centifolia. African Journal of Biotechnology 10(22): 4564-4573.

Basim, E. and Basim, H. 2003. Antibacterial activity of Rosa damascena essential oil. Fitoterapia 74(4): 394-396.

Bruneau, A., Starr, J.R. and Joly, S. 2007. Phylogenetic relationships in the Genus Rosa: New evidence from chloroplast DNA sequences and an appraisal of current knowledge. Systematic Botany 32(2): 366-378.

Chanprame, S., Sontikun, Y., Onwimol, P. and Chanprame, S. 2018. Shoots Induction from In Vitro Node of Teak. King Mongkut’s Agricultural Journal 36(2): 126-134. (in Thai)

Chumpookam, J., Kaewnabon, K. and Chanchula, N. 2017. In vitro micropropagation of ‘Chiangmai 60’ and ‘Vietnam GQ2’ mulberry. Thai Journal of Science and Technology 5(3): 273-282. (in Thai)

Gholamhoseinian, A., Shahouzehi, B., Joukar, S. and Iranpoor, M. 2012. Effect of Quercus infectoria and Rosa damascena on lipid profile and atherosclerotic plaque formation in rabbit model of hyperlipidemia. Pakistan Journal of Biological Sciences 15(1): 27-33.

Gholamhoseinian, A., Shahouzehi, B. and Sharififar, F. 2010. Inhibitory effect of some plant extract on pancreatic lipase. International Journal of Pharmacology 6(1): 18-24.

Gholamhoseinian, A., Fallah, H. and Sharififar, F. 2009. Inhibitory effect of methanol extract of Rosa damascena Mill. Flowers on aglucosidase activity and postprandial hyperglycemia in normal and diabetic rats. Phytomedicine 16(10): 935-941.

Gogoi, G., Borua, P.K. and Al-Khayri, J.M. 2017. Improved micropropagation and in vitro fruiting of Morus indica L. (K-2 cultivar). Journal of Genetic Engineering and Biotechnology 15(1): 249-256.

Hameed, N., Shabbir, A., Ali, A. and Bajwa, R. 2006. In vitro micropropagation of disease free rose (Rosa indica L.). Mycopathology 4(2): 35-38.

Horn, W.A.H., Schlegel, G. and Lerstuhl, K.J. 1992. Micropropagation of roses (Rosa hybrida). Acta Horticulturae 226: 623-627.

Jafari, M., Zarban, A., Pham, S. and Wang, T.J. 2008. Rosa damascena decreased mortality in adult Drosophila. Journal of Medicinal Food 11(1):9-13.

Jala, A. and Paitoon, S. 2013. Micropropagation of Dionaea muscipula by tissue culture. Thai Journal of Science and Technology 2(2): 134-139. (in Thai)

Kachonpadungkitti, Y. and Jala, A. 2014. Influence of BA and NAA on inducing new shoots and roots in Bacopa monaneiri (L.) Pennel in vitro. Thai Journal of Science and Technology 3(1): 7-14. (in Thai)

Kachonpadungkitti, Y. and Jala, A. 2016. Influence of BA, IAA, 2,4-D and Kinetin on micropropagation of Hylocereus undatus from segments of hypocotyl and true leaf. Thai Journal of Science and Technology 4(2): 147-154. (in Thai)

Kaewpiboon, C. and Boonnak, N. 2019. Effect of plant growth regulators on the grape callus induction and growth for secondary metabolite production. Thai Science and Technology Journal 27(4):675-683. (in Thai)

Khamrit, R. and Preeman, J. 2019. In vitro surface sterilization and shoot induction from the rhizome of Zingiber montanum. Khon Kaen Agriculture Journal 47(1) (Suppl.): 1393-1398. (in Thai)

Khosravi, P., Kermani, M.J., Nematzadeh, G.A. and Bihamta, M.R. 2007. A protocol for mass production of Rosa hybrida cv. Iceberg through in vitro propagation. Iranian Journal of Biotechnology 5(2): 100-104.

Laohavisuti, N., Ruangdej, U., Seesanong, S. and Wangwibulkit, S. 2018. Effect of disinfectants and plant growth regulator in aquatic plant Bucephalandra sp. Micropropagation. King Mongkut’s Agricultural Journal 35(2): 95-103. (in Thai)

Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with Tobacco tissue culture. Physiologia Plantarum 15(3): 473-497.

Nak-Udom, N., Kanchanapoom, K. and Kanchanapoom, K. 2009. Micropropagation from cultured nodal explants of rose (Rosa hybrida L. cv. ‘Perfume Delight’). Songklanakarin Journal of Science and Technology 31(6): 583-586.

Naquvi, K.J., Ansari, S.H., Ali M. and Najmi, A.K. 2014. Volatile oil composition of Rosa damascene Mill. (Rosaceae). Journal of Pharmacognosy and Phytochemistry 2(5): 130-134.

Palee, J. 2016. Natural propagation and micropropagation of Tupistra albiflora K. Larsen. Science and Technology RMUTT Journal 6(2): 1-16. (in Thai)

Pati, P.K., Rath, S.P., Sharma, M., Sood, A. and Ahuja, P.S. 2006. In vitro propagation of rose: A review. Biotechnology Advance 24(1): 94-114.

Prasertsongskun, S. 2016. In vitro propagation of Etlingera elatior. Journal of Yala Rajabhat University 10(2): 21-28. (in Thai)

Pumisutapon, P., Saisueb, S. and Rodpradit, S. 2018. Studies on surface sterilization and callus induction in Selaginella. Agricultural Science Journal 49(1)(Suppl.): 270-272. (in Thai)

Razavizadeh, R. and Ehsanpour, A.A. 2008. Optimization of in vitro propagation of Rosa hybrida L. cultivar Black Red. American-Eurasian Journal of Agricultural Environment Science 3(1): 96-99.

Ren, W., Qiao, Z., Wang, H., Zhu, L. and Zhang, L. 2003. Flavonoids: promising anticancer agents. Medicinal Research Reviews 23(4): 519-534.

Sharma, N.K., Choudhary, R.C. and Kumar, M. 2014. An improved plant regeneration system of Ocimum sanctum L- An important Indian holy basil plant. Journal of Cell Tissue Research 14(1): 4143-4148.

Somsong, U. 2013. Tissue culture of citrus medica L. var. linetta Risso. Prawarun Agricultural Journal 10(1): 29-37. (in Thai)

Srisawang, A., Laohavisuti, N. and Ruangdej, U. 2019. The optimum sterilization procedures on micropropagation of Anubias sp. ‘White’. King Mongkut’s Agricultural Journal 37(4): 648-654. (in Thai)

Sukkeaw, T., Ramasoot, S. and Parinyapong Chareonsap, P. 2016. Microprapagation of Black Bat Flower. Wichcha Journal 35(2): 33-40. (in Thai)

Topoonyanont, N., Audtama, N. and Kaewdam, T. 2019. Factors affecting micropropagation system of strawberry cv. Praratchatan 80 and cv. 329. Thai Journal of Science and Technology 8(2): 176-189. (in Thai)

Tudses, N., Phungbunhan, K. and Audta, L. 2019. Effects of BA and NAA on growth and development of Musa sapientum (ABB group) cv ‘Kluai Hin’ in vitro and effects of substrate on growth in vivo. King Mongkut’s Agricultural Journal 37(2): 262-273. (in Thai)

Zeinali, H., Tabaei-Aghdaei, S.R. and Arzani, A. 2009. A study of morphological variations and their relationship with flower yield and yield components in Rosa damascena. Journal of Agricultural Science and Technology 11(4): 439-448.