Isolation of Secondary Metabolites-Producing Endophytics and Free Living Microorganisms from Sea Active against Methicillin-Resistant Staphylococcus aureus
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Abstract
This study is a part of drug-based screening from natural products. The sponges and mud from the sea were randomly collected for antibiotic-producing bacteria isolation. The bacterial isolates mud from the sea and endophytic from sponges found on Ko Sarai area, Satun province. They were tested for antibiotic-sensitive bacteria (Staphylococcus aureus TISTR517) and methicillin-resistance bacteria (Staphylococcus aureus MRSA142). Double layer overlay and agar well diffusion methods were tested. From the experiment, 11 isolates bacteria were separated from the endophytic group that covered the surface of the sponge and free-living bacteria groups. The morphology of isolated bacteria showed embedded colony which acted as Actinomycetes. Isolated bacteria code S6.2 and SK3 showed antimicrobial against two bacterial strains tested. This research may be useful for methicillin resistant antibiotic studies in the future.
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