Isolation of Secondary Metabolites-Producing Endophytics and Free Living Microorganisms from Sea Active against Methicillin-Resistant Staphylococcus aureus

Main Article Content

Monthon Lertcanawanichakul
Pachara Pedpradab


This study is a part of drug-based screening from natural products.  The sponges and mud from the sea were randomly collected for antibiotic-producing bacteria isolation. The bacterial isolates mud from the sea and endophytic from sponges found on Ko Sarai area, Satun province. They were tested for antibiotic-sensitive bacteria (Staphylococcus aureus TISTR517) and methicillin-resistance bacteria (Staphylococcus aureus MRSA142). Double layer overlay and agar well diffusion methods were tested. From the experiment, 11 isolates bacteria were separated from the endophytic group that covered the surface of the sponge and free-living bacteria groups. The morphology of isolated bacteria showed embedded colony which acted as Actinomycetes. Isolated bacteria code S6.2 and SK3 showed antimicrobial against two bacterial strains tested. This research may be useful for methicillin resistant antibiotic studies in the future.

Article Details

How to Cite
Lertcanawanichakul , M., & Pedpradab, P. (2020). Isolation of Secondary Metabolites-Producing Endophytics and Free Living Microorganisms from Sea Active against Methicillin-Resistant Staphylococcus aureus. Rajamangala University of Technology Srivijaya Research Journal, 12(3), 399–407. Retrieved from
Research Article
Author Biographies

Monthon Lertcanawanichakul , Walailak University

School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand.

Tropical Medicine Research Unit, Research Institute for Health Sciences, Walailak University 80160, Thailand.

Pachara Pedpradab, Faculty of Sciences and Fisheries Technology, Rajamangala University of Technology Srivijaya.

Faculty of Sciences and Fisheries Technology, Rajamangala University of Technology Srivijaya, Trang Campus, Sikao, Trang 92150, Thailand.


Anand, T., Abdul, W.B., Yogesh, S., Shouche, U.R., Jay, S. and Siddhartha, P.S. 2005. Antimicrobial Activity of Marine Bacteria Associated with Sponges from the Waters of the Coast of South East India. Microbiological Research 16: 252-262.

Blunt, J.W., Copp, B.R.M., Munro, M.H.G., Northcote, P.T. and Prinsep, M.R. 2004. Marine Natural Products. Natural Product Reports 21: 1-49.

Colwell, R.R. 2002. Fulfilling the Promise of Biotechnology. Biotechnology Advances 20: 215-228.

Dechsakulwatana, C. and Putchakarn, S. 1998. Research Report on Studies on Bioactive Metabolites from Marine Bacteria which Associated with Thai Sponges. National Research Council of Thailand. (In Thai)

Ely, R., Supriya, T. and Naik, C.G. 2004. Antimicrobial Activity of Marine Organisms Collected of the Coast of South East India. Journal of Experimental Marine and Ecology 339: 121-127.
Fenecal, W. and Jensem, P.R. 1993. Marine microorganisms: A new Biomedical Resource, pp. 500. In Attaway, D.H. and Zaborsky, O.R., eds. Marine Biotechnology, I. Pharmaceutical and bioactive natural products, Plenum press, New York.

Friedrich, A.B., Markert, H., Fendert, T., Hacker, J., Proksch, P. and Hentschel, U. 1999. Microbial Diversity in the Marine Sponge Aplysina caverniucola (formerly Verongia cavernicola) Analyzed by Fluorescence In Situ Hybridization (FISH). Marine Biology 134: 461-470.

Gandhimathi, R., Arunkumar, M., Selvin, J., Thangavelu, T., Sivaramakrisnan, S., Kiran, G.S., Shanmughapriya, S. and Natarajseenivasan, K. 2008. Antimicrobial Potential of Sponge Associated Marine Actinomycetes. Journal of Medical Mycology 18: 16-22.

Groombridge, B. 1992. Global Biodiversity: Status of the Earth’s Living Resources. Chapman and Hall, London.

Menezes, C.B., Bonugli-Santos, R.C., Miqueletto, P.B. Passarini, M.R., Silva, C.H., Justo, M.R., Leal, R.R., Garboggini, F.F., Oliverira, V.M., Berlinck, R.G. and Sette, L.D. 2010.
Microbial Diversity Associated with Algae, Ascidians and Sponges from the North Coast of Sao Paulo State, Brazil. Microbiological Research 165: 466-482.

Prapaiwong, A. 2012. Isolation of Bacteria with Antimicrobial Activity from Marine Invertebrates. Thesis for the Degree of Master of Science in Microbiology. Department of Microbiology, Faculty of Science, Prince of Songkla University.

Proksch, P., Edrada, R.A. and Ebel, R. 2002. Drugs from the Sea-Current Status and Microbiological Implications. Applied Microbiology and Biotechnology 59: 125-134.

Shetty, P.R., Buddana, S.K., Tatipamula, V.B., Naga, Y.V.V. and Ahmad, J. 2014. Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activity. Brazilian Journal of Microbiology 45(1): 303-312.

Wilson, J.E. 1988. The Systematics’ Perspective, pp. 1-6. In Fautin, D.G., ed. Biomedical Importance of Marine Organisms. California Academy of Science, San Francisco.