Xylanase Production by Candida glabrata Strain SKa4/2 using Agricultural Residues
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Abstract
Xylanase production from Candida glabrata strain SKa4/2 in the medium containing yeast extract as nitrogen source at the different concentration of 0.5 and 1% and carbon sources from glucose or Birchwood xylan at the concentration of 1% was investigated. The result showed that this yeast produced the highest yields of xylanase when grew in the medium containing 1% yeast extract and Birchwood xylan as a carbon source with the enzyme activity of 0.89 UmL-1. Among the different concentration of Birchwood xylan (0.5-1.5%) on xylanase production, the maximum enzyme activity was achieved in the medium containing 1% Birchwood xylan (0.85 UmL-1). Amongst different agricultural waste samples (such as sugarcane bagasse, rice straw and corn husk) as a carbon sources, sugarcane bagasse gave the highest yields of xylanase and the activities was 0.61 UmL-1. This study suggests that sugarcane bagasse could be utilized as a carbon source for economical xylanase production by Candida glabrata strain SKa4/2. This may in turn reduce the cost of enzyme production leading to efficient use of lignocellulosic materials to produce value-added products.
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