Potency of Natural Phage in Pseudomonas aeruginosa Lysis
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Abstract
A phage, also known as a bacteriophage, is a bacteria's natural predator. Phages can sustain themselves by relying on bacteria to increase their numbers. Many pathogenic bacteria are now resistant to antibiotics. Therefore, phages can be used as an alternative method to kill antibiotic-resistant bacteria. Pseudomonas aeruginosa is an antibiotic-resistant bacterium because it can produce biofilm, which contributes to more resistance than other bacterial species. In this study, a natural phage capable to destroy P. aeruginosa was isolated. It was found that the shape of the PAMFUP2 lytic phage under a transmission electron microscope belonged to the Myoviridae family. It had a lytic life cycle that was able to destroy P. aeruginosa by causing a clear zone lysis. The phage PAMFUP2 was specific to P. aeruginosa TISTR 1287 strain in a specificity test. It was also able to inhibit P. aeruginosa TISTR 357, but not the other bacterial strains. The phage PAPMFU2 less than 20% could destroy P. aeruginosa TISTR 1287 when incubated in a solution with a pH value of 3 and a temperature of at 70°C. The stability of destructive capacity was maintained when the pH solution was in the range of 3-12. Additionally, a single PAMFUP2 phage could inhibit bacteria growth for up to 7 hours. Phage PAPMFU2 significantly (P < 0.01) inhibited biofilm formation of P. aeruginosa TISTR 1287. Based on the above characteristics, natural phage PAMFUP2 could be used to destroy P. aeruginosa in the future.
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