Restriction Endonuclease Pattern of Thai Bombyx mori L. Nucleopolyhedrovirus

Abstract

Bombyx mori nucleopolyhedrovirus (BmNPV) is a causative agent of grasserie which is the most destructive disease of silkworm (Bombyx mori. L.)  Restriction profiel of Thai BmNPV destructive disease of silkworm (Bombyx mori. L.)  Restriction profile of Thai BmNPV genomic DNA was studied by cleaving the DHA with BamHI, Bg/II, HindIII, Ncol, Pstl, and the minimize of 6, 11, 19, 11 and 17 different DNA fragments wee produced, respectively.   Molecular size of the fragments was estimated by summation of the size of DNA fragments generated by restriction exzymes degestion as viewed on the electrophoresed get.  The Thai BmNPV genome was estimated to be in the range of 92.3-125.8 kb with the average of 110.6 kb.  In comparison with genome of BmNPV T3 and D1 isolates from Japan, and N isolate from India, the Thai BmNPV genome is the smallest in size.