In Vitro Propagtion of Venus' Slipper (Paphiopedilum godefroyae (Godefr.) Pfitz.)

Abstract

In vitro propagation of Paphiopedilum godefroyae (Godefr.) Pfitz. was inducted the large number of multiple shoots and conducted at the laboratory of Biotechnology Research and Development Office during 2001-2006. The experiment was divided into two trials. The frist one was studied on the effects of growth regulator of BA (6-bebzyladenine) and NAA (naphthaleneacetic acid) on the plantlets development. The experiment was designed s 4x5 Factorial in CRD with 5 replications. The plantlets were cultured on Murashige and Skoog (MS,1962) medium suplemented with 0, 1, 2 and 3 mg/I BA and 0, 011, 0.3,0.5 and 0.7 mg/I NAA. After 8 months the best growth and development of plantlets was obtained in cultured of MS + BA 2 mg/I + NAA 0.5 mg/I media. The result was completely supported vigorous leaves, roots and 3 shoots per plant initiation. The second trial was studied on colnal propagation or root part and was designed as CRD with 10 replications and 3 treatments. The diameter of expansion roots 2, 3 and 4 cm of 8 months old were cultured on MS+BA 2 mg/I + NAA 0.5 mg/I media. After 10 months in culture, the expansion root 4 cm of diameters gave the highes tnumber at 38.9 shoots per root. The shoots were expcised and cultured on MS+BA 2 mg/I NAA 0.5 mg/I + activated charcoal 2 gm/I media to develop the roots and vigorous plantlets into the large numbers for propagation and conservation under natural condition.