Identiffication of Durian (Durio spp.) Cultivars, clones by DNA Amplification Fingerprinting (DAF)

Abstract

The polymerase chain reaction (PCR) – based DNA amplification fingerprinting (DAF) approach was used to investigate genetic relationships among 9 species of genus Durio, 56 cultivars of common durian (D.zibethinus Murr.) Pairwise similarity matrix were developed using band-sharing data generated by the selected 12 primers out of 180 primers such as Operon A-04, A-06, A-09, A-19, A-20, B-01, B-03, B-16, C-06, G-20, S-01, and S-02. These experiments were conducted at Chanthaburi Horticultural Research Center and Horticulture Research Institute between October 1999- September 2005. In the first experiment with 8 spccies and 18 cultivars, there are 187 polymorphic loci from 217 loci or 86.17% for species and cultivars identification. Second experiment with 9 species and 56 cultivars, 278 polymorphisms were identified from a total of 298 produced bands or 93.29%. The third experiment, 48 polymorphic loci from 192 loci or 25.00% were identified for selected clones of 4 commercial cultivars. Dendrograms were constructed using the unweighted pair group method with arithmetic averages (UPGMA), could separate the genotypes of species and cultivars into 4 and 3 groups, and the genotypes of selected clones into 4 groups for these experiments, respectively.