Identification of Selected F1 hybrid durians as promising lines by DNA Amplification Fingerprinting (DAF)

Abstract

The polymerase chain reaction (PCR) – based DNA Amplification Fingerprinting (DAF) approach was used to investigate genetic relationships among F₁ hybrid durians and their parents including promising lines. Pair wise similarity matrix were developed using band sharing data generated by the selected 12 out of 180 primers such as Operon A-04, A-06, A-09, A-19, A-20, B-01, B-03, B-16, C-06, G-20, S-01 and S-02. These experiments were conducted at Chanthaburi Horticultural Research Centre and Horticulture Research Institute between October 1999 – September 2005. In the first experiments with 5 F₁ hybrids from the CN x MT cross, there are 31 polymorphisms from a total of 118 produced bands or 26.27%. Second experiment with 120 F₁ hybrids from 24 crosses, 166 polymorphisms were identified from a total of 276 produced bands or 60.14%. The third experiments, 156 polymorphic loci from 298 loci or 52.35% were evaluated for 29 promising F₁ hybrids identification. Dendrograms were constructed using the unwished pair group method with arithmetic averages (UPGMA), could separate the genotypes of F₁ hybrids, promising F₁ hybrids and their parents into 4, 2 and 3 group for these experiments, respectively.