Tissue Culture of Leuang Pratew 123 and Khao Tah Haeng 17 Native Thai Indica Rices for Biotechnological Breeding

Abstract

The two native biotechnological Thai lndica rices, Leuang Pratew 123 and Khao Tah Haeng 17 were cultured from mature seeds in vitro biotechnological breeding. Embryogrnic calli were induced by culture on modified R 2 medium supplemented with 2 mg/l 2,4-D. The proliferation of these calli (0.07909 g/seed/3 weeks) was cultured on MS modified medium supplemented with 2 mg/l 2,4-D, 1g/l casein hydrolysate and 1g/l L-proline. The maximum regeneration (28.53 plantlets/treatment) was dehydrated for 4 days, then transferred to the plant regeneration medium (MS basal medium supplemented with 1 mg/l kinetin, 0.5 mg/l NAA, 0.5 mg/l BA , 3 mg/l BA, 0.5 g/l casein hydrolysate and 1g/l L-proline).The young plantlets were grown on MS basal medium and more than 90% survival, they were transplanted in the soil.