Insecticide Resistance of the Green Leafhopper, Nephotettix cincticeps, to Systemic Insecticides by “Parafilm Test”: a Simple Method to Evaluate the Efficiency of the Insecticide

Abstract

The insecticide susceptibility of the green leafhopper, Nephotettix cincticeps to systemic insecticides, imidacloprid, cartap, propaphos and disulfoton, were determined by a newly developed testing method, the”parafilm test” using an artificial membrane to give an artificial diet composed of 2.5% sucrose and series dilution of insecticies. This test can measure both mortality (LC50) and feeding inhibition (FI50). When the three local populations of green leafhopper with different resistance levels to organophosphates and carbamates were compared with LC50 and FI50 obtained by this testing method. Feeding inhibition at sublethal doses was found to be the most remarkable with imidacloprid, followed by cartap without regarding to resistance levels, while feeding inhibition by propaphos and diusulfoton was observed only with the resistant green leafhopper.