Mutation Breeding of Aromatic Rice (Oryza sativa L. var. KDML 105) for Photoperiod Insensitivity by Chemical Mutagen

Abstract

Mutation breding of aromatic rice variety KDML 105 for photoperiod insensitive character was conducted by using chemical mutagen ethyl methanesulfonate (EMS). Seeds were soaked in EMS solution at the concentrations of 0.75, 1.0 and 1.25% for 12 hrs. The treated seeds were then grown into M1 plants and M2 progenies. The M2 progenies were determined for photoperiod sensitivity under long daylength condition. five plants among 3,320 M2 plants was inherited to the M3 progenies when grown under exhibiting photoperiod insensitive character, short culm and physical grain quality similar to KDML 105 were selected. All of the 64 M4 lines wee photoperiod insensitive when grown under long daylength condition. Grain physical quality, cooking and eating quality including aroma of the M4 lines were analysed. Eleven M4 lines possessing grain quality similar to KDML 105 were selected and 25 plants showing good agronomic characters were chosen from these lines. All of the 25 M5 lines showed photpperiod insensitive character when grown under short daylenght condition. Seeds of each line were analysed for grain physical quality, cooking and eating quality including aroma, Eight M5 lines having grain qualigy similar to KDML 105 and good agronomic performance were selected. Yield test and grain