Characterization of Xanthomonas causing of Bacterial Leaf Spot of Tomato and Pepper in Thailand
DOI:
https://doi.org/10.14456/thaidoa-agres.2020.7Keywords:
Xanthomonas, bacterial leaf spot disease, tomato, pepper, multilocus sequence, housekeeping genesAbstract
Bacterial leaf spot disease of tomato and pepper is the importance disease in Thailand especially to seed exporting industry. Xanthomonas spp. associated with bacterial leaf spot disease of tomato and pepper were reclassified into four species, namely X. euvesicatoria, X. gardneri, X. perforans, and X. vesicatoria, according to Jones et al., (2004). To assess and update the bacterial pathogens of tomato leaf spot disease of tomato and pepper, 38 bacterial isolates associated with leaf spot disease collected from north, north-east and central part of Thailand which identified as Xanthomonas spp. base on general characteristics of morphology, physiological and biochemical properties, were characterized. Eighteen isolates are selected for further determination by multilocus sequence analysis (MLSA) of gyrB, efp, dnaK and atpD genes and PCR amplification using species-specific primer sets. Based on the characterized results, two species were identified, most of tomato isolates were X. perforans and the others, 10 isolates from pepper as X. euvesicatoria. No X. gardneri and X. vesicatoria were found in this study. However, no PCR products were obtained from all isolates which identified as X. perforans by using the species-specific primer pair Bs-XpF/ Bs-XpR for X. perforans.
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