Effectiveness of Bioproduct of Bacillus subtilis to Control Rice Sheath Blight Disease

Abstract

Bioproducts TRF A and TRF B liquid formulation of antagonistic Bacillus subtilis against rice sheath blight (Rhizoctonia solani Khun.) were developed by Prince of Songkla University, Kasetsart University and Department of Agriculture under the Thailand Research Fund (TRF). The efficacy of both formulation were tested among other bioproducts compared to the standard fungicide Validacin 3% Liq. under greenhouse conditions. CRD of 10 treatments with 7 replications were designed on RD 23 varity. Results indecated that fungicide, Validacin 3% Liq. showed the most effective in suppressing sheath blight disease development, followed by bioprocucts TRF A, TRF B, Larminar WP, Agroguard Liq., Agroguard WP, BCA No.321+562, Subtilar WP and BS 916 Liq. respectively. However, all treatment showed significantly defferent control plots (non application). The top four ranks of bioproducts and fungicide were chosen for further test in field trial, wet season1999 and dry season 2000 of RCB design containing 6 treatments with 4 replications, using RD 23 variety. Results indicated that fungicide, Validacin 3% Liq. showed the most effective followed by bioproducts TRF A, TRF b, Larminar WP. and Agrohuard Liq. respectively. All treatments showed significantly defferent from control plots. In conclusion, results from field trial confirm as those in greenhouse.