Embryogenesis of Citrus reticulata (Keawan and Shokoon)

Abstract

Embryogenic callus can be induced from immature ovule of Citrus reticulata, Thai cultivars : Som Keawan and Som Shokoon. the higher callus induction frequency can be obtained from fruits at 0.6-0.8 cm diameters. The larger ovule sizes showed higher frequency inducting callus than that of the smaller ones. Callus can be induced by two different kinds of media, Murashige & Tucker basal medium + 500 mg./L malt extract + 2 mg./L BA and Basal medium + 500 mg./L malt extract + 1 mg./L kinetin + 0.1 mg./L IAA. Callus can be proliferated in both liquid and solid basal media of Murashige & Tucker. Development media of Som Keawan and Som shokoon were supplemented with 5% galactose or 5% lactose. Somatic embryos can be regenerated with normal shoots and roots in the media containing 1.0 mg./L GA3