Indentification of CaMV the Cause of Mottle an dStunt on Carnation in Thailand

Abstract

Virus diseases of carnation had never been reported in Thailand. Mottle, stunt, yellow and leaf twisted symptoms were found on susceptible varieties of carnation such as pink color variety. The Spherical virus about 28 nm in diameter were detected from infected plants. The virus was transmitted by mechanical method which caused local yellow and necrotic lesion symptom on chenopodium quinoa, C. amaranticolor and Gomphrena globosa, mild mottle symtom on Dianthus caryophyllus var. charming and Paola, yellow at the top, on var. White sin but no symptom on var. Killer and Laguna. The virus gave positive reaction with the antiserum of CaMV by ISEM. Physical property of TIP at 90 ?C in 10 min, DEP at 10-5 and LIV about 50 days at (28 ?C). Absolutely, this virus is identical with CaMV. Purified viruses were prepared from infected C. amaranticolor and immunized a rabbit weekly for 3 times. The antiserum was firstly bled 20 days after the last injection. The purified IgG from the 4th and 5th bleeding of antisera gave positive reaction at dilution of 1:20^4 in ELISA with its IgG-enzyme conjugate at dilution of 1:10^4.