Clonal Propagation and Growth of Banana (Musa (AAA group) Grand Nain) through Tissue culture

Abstract

clonal propagation and growth of banana (Musa (AAA group) Grand Nain) Shoot in vitro culture was studied in this experiment. The shoots with apical meristem were cut into cubic inch, surface sterilized with 10% Clorox for 15 minutes, vertically cut into 4 pieces, cultured on modified MS medium (supplemented with 15% coconut water and 1 mg/1 BA) respectively, It was found that a tissue developed into a shoot and therefore a plantlet in 8 weeks. The plantlets wee longitudianal cut and subcultured on modified MS medium. The plantlets increased approximately 2.44 shoots per plant in 4 weeks. Each plantlet producted by this method costs 7.60 baht.