K2 a Newly Isolated Strain of Bacillus amyloliquefaciens Regulates Responsive Proteins for Its Survival and Promotes Plant Growth of Rice Seedlings against Bacterial Leaf Blight and Salt Stresses

Authors

  • Sasiprapha Marach Department of Plant Pathology, Faculty of Agriculture, Kasetsart University
  • Tiyakhon Chatnaparat Department of Plant Pathology, Faculty of Agriculture, Kasetsart University
  • Supot Kasem Department of Plant Pathology, Faculty of Agriculture, Kasetsart University
  • Sutruedee Prathuangwong Department of Plant Pathology, Faculty of Agriculture, Kasetsart University

DOI:

https://doi.org/10.14456/thaidoa-agres.2020.24

Keywords:

Halophilic bacteria, Phylogenetic analysis, Xanthomonas oryzae pv. oryzae, High-throughput proteome analysis

Abstract

Soil salinity limits the growth and productivity of crop plants worldwide including Thailand. Plant growth promoting rhizobacteria (PGPR) can elicit plant tolerance against both biotic and abiotic (adverse-environmental effects) stresses that may become an alternative process in crop management system. In this study, a new PGPR strain is hypothesized whether it can adapt to high salt concentrations and promote seedling growth of rice against Xanthomonas oryzae pv. oryzae (Xoo), causal organism of bacterial leaf blight, under salt stress that might correlate with its biosynthesis of stress-responsive proteins production. Forty eight bacteria were isolated from different salt -soil lands, and strain K2 (obtained from mangrove plant rhizosphere at Bangkrachao, Samut Prakan) was selected for further investigation based on its superior in biocontrol activity and survival in nutrient broth (NB) plus 12% NaCl which indicated that K2 was a halophilic bacterium. Strain K2 was identified using physiological and biochemical properties, 16s rRNA and gyrB nucleotide sequencing analysis revealed that K2 was placed in Bacillus amyloliquefaciens. Culture filtrates of strain K2 grown in NB with or without 5%NaCl for 24 h were subjected to proteomic analysis. Strain K2 and its culture filtrates exhibited a good performance in Xoo suppression shown by plate assay, and increased growth enhancement of rice seedlings (root length and shoot height) under both normal and salt-stress conditions. Of the shotgun proteomic LC-MS/MS identified proteins, the specific proteins were up-regulated in K2 – salinity grown cells. A key role for proteins predominantly expressed in above analysis was likely mediating plant health tolerated to Xoo and salt stresses. Those involving proteins included the iron scavenging and transport, defense mechanism, the synthesis and transport of compatible solutes, protein expression in protecting K2 survival and adaptation, biocontrol activity, plant growth promotion, and induced salt tolerance was discussed.

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Author Biographies

Sasiprapha Marach, Department of Plant Pathology, Faculty of Agriculture, Kasetsart University

2/Center for Advanced Studies in Tropical Natural Resources, Kasetsart University, Bangkok 

3/Present office at Plant Protection Promotion and Soil-Fertilizer Management Division, Department of Agricultural Extension, Bangkok

Tiyakhon Chatnaparat, Department of Plant Pathology, Faculty of Agriculture, Kasetsart University

2/Center for Advanced Studies in Tropical Natural Resources, Kasetsart University, Bangkok

Supot Kasem, Department of Plant Pathology, Faculty of Agriculture, Kasetsart University

Center for Advanced Studies in Tropical Natural Resources, Kasetsart University, Bangkok

Sutruedee Prathuangwong, Department of Plant Pathology, Faculty of Agriculture, Kasetsart University

Center for Advanced Studies in Tropical Natural Resources, Kasetsart University, Bangkok

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2020-09-28

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Marach, S., Chatnaparat, T., Kasem, S. ., & Prathuangwong, S. (2020). K2 a Newly Isolated Strain of Bacillus amyloliquefaciens Regulates Responsive Proteins for Its Survival and Promotes Plant Growth of Rice Seedlings against Bacterial Leaf Blight and Salt Stresses. Thai Agricultural Research Journal, 38(3). https://doi.org/10.14456/thaidoa-agres.2020.24

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