Identification of Quantitative Trait Loci (QTL) for Rust Disease Resistance in Fresh-market Corn Population
DOI:
https://doi.org/10.14456/thaidoa-agres.2021.1Keywords:
Corn, Puccinia polysora, Genome Wide Association studyAbstract
Southern rust disease caused by Puccinia polysora Underw. is the main problem of corn production in Thailand. The objectives of this study were screening resistance varieties of corn to P. polysora and identifying quantitative trait loci (QTL) associated with the resistance. Two isolates of P. polysora were morphologically characterized, and sequentially analyzed in the ITS rDNA region. Results revealed that both isolates could be grouped with P. polysora from Genbank (NCBI) with 99% bootstrap supporting. Resistance evaluation of 66 lines of sweet corn and waxy corn against these two isolates found that Bio18-1 and Bio18-35 lines were highly resistance. They were suitable candidates for further investigation of resistant sources to corn rust disease. In addition, we found that each inbred line showed different degrees of resistance and susceptibility to P. polysora collected from different sources. Therefore, more than one fungal isolates should be used in the breeding programme for corn rust resistance in Thailand to ensure that resistance obtained are effective against wide range of isolates present in the country. To identify genomic regions associated with rust resistance in maize, a genome-wide association analysis (GWAS) was conducted using 159,201 single-nucleotide polymorphism (SNP) markers of the 66 corn lines. Results showed that the two QTLs significantly associated with rust resistance were on chromosome 10 which was against Chiang Mai isolate and onchromosome3 which was against Nakhon Ratchasimaisolate. For futureanalysis, SNP markers and haplotype associated with 2 QTLs will be developed and evaluatedfor rust resistance in corn breeding programme.
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