Toward the Identification of Dacini Fruit Flies (Diptera : Tephridae) in Thailand Using Phylogenetic and Wing Morphometrics Analyses


  • Yuvarin Boontop Plant Protection Research and Development Offifice, Department of Agriculture
  • Chamaiporn Buamas Plant Protection Research and Development Offifice, Department of Agriculture
  • Kessuda Sonsiri Plant Protection Research and Development Offifice, Department of Agriculture
  • Jomsurang Duangthisan Plant Protection Research and Development Offifice, Department of Agriculture
  • Sitisarodom Kaewsawat Plant Protection Research and Development Offifice, Department of Agriculture



fruit fly, morphometrics, phylogenetics


Tropical fruit flies (Tephritidae: Dacinae: Dacini) are among the world’s worst horticultural pests and are of particular biosecurity⁄ quarantine concern. Species of Dacini are notoriously difficult to identify using morphology, which, in view of their economic and international trade importance, creates ongoing difficulties for plant protection. The goal of this research was to perform phylogenetic and geometric
morphometric analyses to support definition of six species of importance to Thailand i.e Bactrocera correcta, B. carambolae, B. dorsalis, B. latifrons, Zeugodacus cucurbitae and Z. tau. Materials were collected from various kinds of fruit orchards and by lure trapping in all regions in Thailand during 2017 - 2018. Phylogenetic analyses of Cytochrome c oxidase subunit I (cox1) DNA sequences of the six species were performed, Maximum Likelihoodand Bayesianinferenceof sequencesgaveconsistent results. Zeugodacus formed a reciprocally monophyletic sister group to a larger clade comprising the four species of Bactrocera. Wing morphometrics was based on 15 venation landmarks. Centroid sizes computed from landmark data differed significantly among sampled species (P < 0.05). Centroidsize was smallest for B. latifrons (5.53 ± 0.08 mm) andlargest for Z. tau (6.40 0.09 mm). Canonical Variate Analysis (CVA) revealed a clear pattern in which species of Zeugodacus clustered together and were clearly separated from the cluster comprising species of Bactrocera. Individuals of each species clustered together with limitedoverlapping with congeneric species. The present study provides a basis for accurate identificationof the mosteconomicallyimportant Dacini inThailand,usinginternationalstandards and best practice. However, further study is needed, including incorporation of data from additionalgenes toidentify thenumerous membersof Dacinipresent inThailandandneighbouring countries and to characterzie these species so that they can be included in a comprehensive diagnostic system.


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ยุวรินทร์ บุญทบ ศิริณี พูนไชยศรี ชลิดา อุณหวุฒิ ลักขณา บำรุงศรี และสิทธิศิโรดม แก้วสวัสดิ์. อนุกรมวิธานแมลงวันผลไม้สกุล Bactrocera จากสารล่อแมลงในเขตภาคใต้ของประเทศไทย. หน้า 1742-1758. รายงานการประชุมประจำปี 2554. กรมวิชาการเกษตร. กรุงเทพ.

ยุวรินทร์ บุญทบ ณัฏฐิมา โฆษิตเจริญกุล ชมัยพร บัวมาศ และ จารุวัตถ์ แต้กุล. 2562. การจำแนกชนิดแมลงวันผลไม้ศัตรูพืชในกลุ่ม Bactrocera dorsalis complex (Diptera: Tephritidae) ในประเทศไทยด้วยลักษณะทางพันธุกรรม. หน้า 16-29 ใน การประชุมวิชาการ อารักขาพืชแห่งชาติครั้งที่ 14 ระหว่างวันที่ 12 - 14 พฤศจิกายน 2562 โรงแรมดุสิตธานีหัวหิน จังหวัดเพชรบุรี.

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How to Cite

Boontop ย., Buamas ช. ., Sonsiri เ. ., Duangthisan จ. ., & Kaewsawat ส. . (2021). Toward the Identification of Dacini Fruit Flies (Diptera : Tephridae) in Thailand Using Phylogenetic and Wing Morphometrics Analyses . Thai Agricultural Research Journal, 39(2).



Technical or research paper