Study on Phylogenetic Relationships of Thai Ilex L. (Aquifoliaceae) Based on DNA Data
DOI:
https://doi.org/10.14456/thaidoa-agres.2022.1Keywords:
Aquifoliaceae,, DNA barcode, Thai Ilex, morphological classification, phylogenetic relationshipsAbstract
Species boundaries among Ilex L. (Aquifoliaceae) are often not clear-cut due to the morphological overlap of related species. Genetic differentiation is thus needed to clarify their relationship. In the present study, we aimedto investigate the phylogenetic relationships of Thai Ilex species using DNA barcode technique. The DNA barcodes of matK, rpl32-trnL, trnL-trnF and ITS were examined and analyzed using Maximum Parsimony method. A total of 54 DNA sequence data obtained from this study representing 14 species of Thai Ilex. The phylogenetic trees resulted in five congruent main clades with morphological classification. Subgenus Aquifolium Section comprised I. odorata and I. pubifructa whereas the sectional placement for I. depressifructu was still unclear. Subgenus Byronia Section Byronia comprised I. cymosa, I. wallichii, Ilex sp. (1) andIlex sp. (2); Section Lioprinus comprised I. phanganensis and I. umbellulata; Section Paltoria comprised only I. triflora. Subgenus Prinos Section Micrococca comprised only I. micrococca. The results confirmed species identification based on morphology. The placements in the phylogenetic trees supported grouping classification for I. depressifructu, I. phanganensis and I. pubifructa which were recently described as new species for Thailand and could be used as guideline for identifying the two unknown species. More representative species of Ilex and variable DNA barcodes should be added to resolve the indefinable information from the present study and to complete research on diversity of Aquifoliaceae in Thailand.
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