Inducing Substances and Plant Defense Mechanisms Related to Pest Resistance in Tobacco with Scopoletin Extracts from the Morinda citrifolia L. (Noni) Fruits
DOI:
https://doi.org/10.14456/thaidoa-agres.2025.25Keywords:
scopoletin, noni fruit, extraction, resistance, plant elicitorAbstract
Scopoletin is a secondary metabolite found in many plants. It possesses antioxidant properties and inhibits the growth of microorganisms. This research aimed to extract scopoletin from Thai noni (Morinda citrifolia L.) fruit to investigate its potential role in inducing compounds and mechanisms related to pest resistance in tobacco plants. The extraction was performed using the maceration method with ethanol as the solvent. The extract was then separated by column chromatography using silica gel as the stationary phase and a mixture of ethyl acetate and hexane (0–60% v/v) as the mobile phase. The presence of scopoletin in each fraction was detected by TLC under UV light at 365 nm. The fraction containing scopoletin was further purified using 2% methanol in dichloromethane as the mobile phase. The purified compound was identified using TLC, UV-visible spectroscopy, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. The results confirmed that the obtained yellow crystal were scopoletin, with a yield of 0.038±0.01%. A resistance induction assay was performed by infiltrating scopoletin into tobacco leaves at concentrations of 100, 500 and 1,000 μM. The treatment stimulated the production of salicylic acid and increased the activities of phenylalanine ammonia-lyase, glucanase and peroxidase, with peak responses observed at different time points. These findings suggest that scopoletin isolated from Thai noni fruit may functions as a plant elicitor that enhances pest resistance in tobacco by activating signaling molecules and defense-related enzymes.
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