Development of GLIFT Kit for Detection of Sugarcane Mosaic Virus subgroup Maize Dwarf Mosaic Virus

Abstract

Gold labeled IgG flow test (GLIFT) was developed as test kit for Sugarcane mosaic virus (SCMV) detection based on serology and lateral flow technique. Accuracy, convenience and rapid detection are the novel properties of this technique. The GLIFT kit will be very useful for varietal screening in breeding program, forecasting and early warning of Sugarcane mosaic virus epidemic. The SCMV IgG was purified and tested by dot immunobinding assay (DIBA) to obtain the proper concentration for using in GLIFT kit. IgG was conjugated with colloidal gold and amount of 100-120 μμl (6.6 μμl/cm) of IgG conjugated gold were used for lining on 15 –18 cm conjugated release pad Control line and test line were made on nitrocellulose membrane using 40 μμl (2.2 μμl/ cm) of goat anti-rabbit IgG and SCMV IgG respectively. The appearance of color on both test line and control line indicated positive result. In case of negative result, no color was shown on the test line. GLIFT kit were tested for their efficacy to detect SCMV from infected corn leaves. Satisfactory result was obtained with color gradually appeared on control line and test line within 5-10 minutes.