Standard DNA Construction for Genetically Modified Soybean Detection

Abstract

Detection of genetically modified soybeans in the laboratory at all times needed using of a control reference for the analysis. The invention of standard DNA in plasmids form for using in the analysis of genetically modified soybeans was created at the Biotechnology Research and Development office, the Department of Agriculture during October 2007- September 2009 by amplifying DNA fragments of CP4EPSPS genes of genetically modified soybeans and soybean Lectin genes of non-genetically modified soybeans. The components of the two genes are ligated to the pCR8/GW/TOPO plasmid. It has been called “pStdDOA/GMO1” standard DNA. When it was put to the accuracy test it was found that the pStdDOA/GMO1 standard DNA was a correctly and effective reference material. It can be used for analysis of genetically modified soybeans. The alternative is for replacing the importion of expensive reference materials from overseas and it can reduce cost of samples analysis.