Effects of Thidiazuron (TDZ) และ 2, 4-Dichlorophenoxyacetec acid (2, 4-D) for Induced Callus and Shoots of Poodjeed (Tabernaemontana divaricate L.) in vitro Culture

Abstract

A study of tissue culture was aimed to increase the number of poodjeed (Tabernaemontana divaricate L.) “Crape jasmine” or “Pinwheel jasmine”. The study was made in vitro culture of young leaf as leaf explants at the Department of Biology. University of Phayao durning September to December, 2001. The leaf explants were surface sterilization with sodium hppochloride (NaOCI) at 10 % for 10 minutes and followed with NaOCI again at 5% for 5 minutes which sterilized leaf explants and growth for 80 %. The sterilized leaf explants were cultured on modified MS medium supplemented with thidiazuron (TDZ) at 0,0.2,0.3,0.4 and 0.5 mg/l combination with 2,4-Dichlorophenoxyacetic acid (2, 4-D) at 0.1 mg/l. The result showed the modified MS medium with TDZ at 0.4 and 0.5 mg/l. combination with 2, 4-D at 0.1 mg/l were the best for induction of callus and shoots via mean number 2.60 and 3.00 shoots/explants respectively after being cultured for 3 months.