Antibody Against Actinophages Isolated from Soil in Thailand

Authors

  • ONANONG PRINGSULAKA Program in Division of Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, THAILAND
  • SURINA CHAVANICH Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, THAILAND
  • KATSUMI DOI Microbial Genetic Division, Institute of Genetic Resources, Kyushu University, JAPAN
  • SEIYA OGATA Microbial Genetic Division, Institute of Genetic Resources, Kyushu University, JAPAN

Keywords:

actinophage, streptomycetes, soil, antibody

Abstract

Streptomycetes and phages were isolated from soil in different locations of Thailand. The isolated phages, distinguished by host range determination, plaque morphology and phage morphology under transmission electron microscope, were partially purified by single plaque isolation. From host range determination in a variety of phages, phage Roi-1 was selected to serve as an antigen for immunization in rabbits. High titers of phage suspension were prepared by removal of host cells passing through 0.45 µm membrane filter. Five milliliters of the pure phage suspension was injected to three rabbits twice a week for 1 month. Sera from rabbits were subsequently collected and tested for cross reactivation against another 8 phages. The results revealed that phage Nsaw-30 gave a high degree of relatedness to phage Roi-1.

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Published

2003-10-01

How to Cite

PRINGSULAKA, O., CHAVANICH, S., DOI, K., & OGATA, S. (2003). Antibody Against Actinophages Isolated from Soil in Thailand. Tropical Natural History, 3(2), 1–6. Retrieved from https://li01.tci-thaijo.org/index.php/tnh/article/view/102839