Systematic Significance of Leaf Anatomical Characteristics in Some Species of Mangifera L. (Anacardiaceae) in Thailand

  • KRITTIN TIPMONTIANE Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, THAILAND
  • ANITTHAN SRINUAL Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, THAILAND
  • WIROT KESONBUA Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani, 34190, THAILAND
Keywords: Leaf Anatomy, Mangifera L., Anacardiaceae, Thailand


The leaf anatomical comparison of some species in genus Mangifera L. from Thailand, such as M. caloneura Kurz, M. camptosperma Pierre, M. duperreana Pierre, M. foetida Lour., M. indica L., M. odorata Griff., M. pentandra Hook.f. and M. qaudrifida Jack in Roxb. was provided. The specimens were investigated by peeling method, clearing method and transverse sections of lamina and petiole from each species of plant, and conducted an examination under light microscope to determine their systematic significance in species delimitation and identification. The consistent anatomical characteristics in all species are i) the typical cyclocytic and staurocytic stomata in adaxial and abaxial surfaces respectively; ii) the amphistomatic leaves; iii) the jigsaw shape with deeply undulate cell wall in adaxial epidermal cell; iv) the presence of sunken peltate trichomes on lamina and midrib; v) the presence of extension in bundle sheath to both surfaces; vi) the presence of fiber at the apex of leaf margin, midrib and petiole; vii) the presence of resin ducts; vii) the presence of mucilaginous cells in epidermis and midrib; and viii) the presence of prismatic crystals in mesophyll, midrib and petiole. In addition, the significantly anatomical features were useful for species delimitation which are as follows; i) the shape of epidermal cell; ii) the outline of leaf margin, midrib and petiole; iii) the shape of epidermal cell at the apex of leaf margin; iv) the layer of hypodermis; v) the number of palisade cell layers in lamina; vi) the presence of grouped fiber below sunken trichome; vii) the distribution of peltate trichome; viii) the presence of sclereid with ramiform-pitted in petiole; ix) the inclusion in each organ e.g. mucilaginous cell, crystal and starch grain; and x) the number of resin ducts in midrib and petiole.


Aderibigbe, A.O., Emudianughe, T.S. and Lawal, B.A.S. 2001. Evaluation of the Antidiabetic Action of Mangifera indica in Mice. Phytotherapy Research, 15: 456-458.
Bbosa, G.S., Lubega, A., Musisi, N., Kyegombe, D.B., Waako, P.,Ogwal-Okeng, J and Odyek, O. 2007. The Activity of Mangifera indica L. Leaf Extracts against the Tetanus causing Bacterium, Closterium tetani. African Journal of Ecology, 45(3): 54-58.
Bibi, H., Afzal, M., Muhammad, A., Kamal, M., Ullah, I. and Khan, W. 2014. Morphological and Anatomical Studies on Selected Dicot Xerophytes of District Karak, Pakistan. American-Eutansian Journal of Agricultural & Environmental Sciences, 14(11): 1201-1212.
Cahyanto, T., Sopian, A., Efendi, M. and Kinasih, I. 2017. The Diversity of Mangifera indica Cultivars in Sabang West Java Based on Morphological and Anatomical Characteristics. Biosaintifika, 9(1): 156-167.
Chandra, D. and Mukherjee, S.K. 2014. Flora of India: Anacardiaceae. Retrieved July 31, 2017, from
Chayamarit, K. 2010. Anacardiaceae. In: Flora of Thailand, vol 10 part 3, (K. Chayamarit, eds.). The Forest Herbarium. Bangkok.
Chayamarit, K. 1997. Molecular Phylogenetic Analysis of Anacardiaceae in Thailand. Thai Forest Bulletin (Botany), 25: 1-13.
Cooper, D. C. 1932. The Development of the Peltate hairs of Shpepherdia Canadensis. American Journal of Botany, 9(5): 423-428.
Cotthem, W.R.J.V. 1970. A Classification of Stomatal Types. Botanical Journal of the Linnean Society, 63: 235-246.
Ferrenberg, S., Kane, J.M. and Milton, J.B. 2014. Resin Duct Characteristics Associated with Tree Resistance to Bark Beetles Across Lodgepole and Limber Pines. Oecologia, 174: 1283-1292.
Franceschi, V.R. and Horner, H.T. 1980. Calcium Oxalate Crystals in Plants. Botanical Review, 46(4): 361-427.
Franceschi, V.R. and Nakata, P.A. 2005. Calcium Oxalate in Plants: Formation and Function. Annual Review of Plant Biology, 56: 41-71.
Ganogpichayagrai, A., Rungsihirunrat, K., Palanuvej, C. and Ruangrungsi, N. 2016. Characterization of Mangifera indica Cultivars in Thailand Based on Macroscopic, Microscopic, and Genetic Characters. Journal of Advanced Pharmaceutical Technology & Reasearch, 7(4): 127-133.
Ganong, W.F. 1895. Present Problems in the Anatomy, Morphology, and Biology of the Cactaceae. Botanical Gazatte, 20(4): 129-138.
Hidayat, T., Pancoro, A. and Kusumawaty, D. 2011. Utility of matK Gene to Assess Evolutionary Relationship of Genus Mangifera (Anacardiaceae) in Indonesia and Thailand. Biotropia, 18(2): 74-80.
Johansen, D.A. 1940. Plant Microtechnique. The Maple Company, New York and London.
Johnson, H. B. 1975. Plant Pubescence: an Ecological Perspective. The New York Botanical Garden, 41(3): 233-258.
Lersten, N.R. and Curtis, J.D. (2001). Idioblast and Other Unusual Internal Foliar Secretory Structures in Scorphulariaceae. Plant Systematics and Evolution, 227: 63-73.
Mckay, S.A.B., Hunter, W.L., Godard, K., Wang, S.X., Martin, D.M., Bohlmann, J. and Plant, A.L. 2003. Insect Attack and Wounding Including Traumatic Resin Duct Development and Gene Expression of (-)-Pinene Synthase in Sitka Spruce. Plant Physiology, 133: 368-378.
Metcalfe, C.R. and Chalk, L. 1957. Anatomy of the Dicotyledons, vol 2. Oxford University Press, London.
Nakata, P.A. 2003. Advances in our Understanding of Calcium Oxalate Crystal formation and Function in Plants. Plant Science 164: 901-909.
Navarro, T. and El Oualidi, J. 2000. Trichome Morphology in Teucrium L. (Labiatae). a Taxonomic Review. Anales del Jardin Botanico de Madrid, 57(2): 277-297.
Norfaizal, M. and Latiff, A. 2013. Leaf Anatomical Characteristics of Bouea, Mangifera and Spondias (Anacardiaceae) in Malaysia. Journal of Life Sciences, 8(9): 758-767.
Parvez, G.M. 2016. Pharmacological Activities of Mango (Mangifera indica): A Review. Journal of Pharmacognosy and Phytochemistry, 5(3): 1-7.
Perpeteuo, G.F. and Salgado, J. M. 2003. Effect of Mango (Mangifera indica L.) ingestion on blood glucose levels of normal and diabetics rats. Plants Foods for Human Nutrition, 58: 1-12.
Phongkrathung, R. and Kermanee, P. 2013. Anatomy and Some Properties of Woods in Mangifera indica L., M. foetida Lour. And M. caloneura Lour. Thai Journal of Botany, 5(Special Issue): 133-141.
Shabani, Z. and Sayadi, A. 2014. The Antimicrobial in Vitro Effects of Different Concentrations of some Plant Extracts including Tamarisk, March, Acetone and Mango Kernel. Journal of Applied Pharmacoceutical Science, 4(5): 75-79.
Shaheen, N., Ajab, M., Yasmin, G. and Hayat, M.Q. 2009. Diversity of Foliar Trichomes and their Systematics Relevance in the Genus Hibiscus (Malvaceae). International Journal of Agriculture & Biology, 11: 279-284.
Sharma, B.G., Albert, S. and Dhaduk, H.K. 2012. Petiolar Anatomy as an Aid to the Identification of Mangifera indica L. Varieties. Notulae Scientia Biologicae, 4(1): 44-47.
Tianlu, M. and Barfod, A. 2008. Flora of China: Anacardiaceae. Retrieved July 31, 2017, from
Wannan, B.S. 2006. Analysis of Generic Relationships in Anacardiceae. BLUMEA, 51: 165-195.
Wannan, B.S. and Quinn, C. J. 1991. Floral Structure and Evolution in the Anacardiaceae. Batanical Journal of the Linnean Society, 107: 349-385.
Yonemori, K., Honsho, C., Kanzaki, S., Eidthong, W. and Sugiura, A. 2002. Phylogenetic Relationships of Mangifera Species Revealed by ITS Sequences of Nuclear Ribisomal DNA and a Possibility of their Hybrid origin. Plant Systematics and Evolution, 231: 59-75.
How to Cite
TIPMONTIANE, K., SRINUAL, A., & KESONBUA, W. (2018). Systematic Significance of Leaf Anatomical Characteristics in Some Species of Mangifera L. (Anacardiaceae) in Thailand. Tropical Natural History, 18(2), 68-83. Retrieved from