DNA Barcoding of Hemerodromia Meigen, 1822 (Diptera: Empididae) from Thailand

  • San Namtaku Department of Biology, Faculty of Science, Mahasarakham University, Kantharawichai District, Maha Sarakham, 44150 THAILAND
  • Adrian R. Plant Division of Research Facilitation and Dissemination, Mahasarakham University, Kantharawichai District, Maha Sarakham, 44150 THAILAND
  • Pairot Pramual Department of Biology, Faculty of Science, Mahasarakham University, Kantharawichai District, Maha Sarakham, 44150 THAILAND
Keywords: cytochrome c oxidase I, DNA barcode, Empididae, Hemerodromia, Thailand

Abstract

Species of Hemerodromia Meigen, 1822 (Diptera, Empididae, Hemerodromiinae) are important components of lotic habitats in freshwater ecosystems. The goal of this study was to test the efficiency of the mitochondrial cytochrome c oxidase subunit I (COI) barcoding region for species level identification of Hemerodromia in Thailand. Twelve Hemerodromia species were collected from 31 sites in North and Northeastern Thailand and 135 COI sequences obtained. DNA barcoding identification analysis based on the best close match method performed well; with 100% of specimens agreeing with morphological identification. A phylogenetic tree based on the 135 mitochondrial barcode sequences obtained here and 28 sequences from the NCBI database revealed a well-supported monophyly for all Hemerodromia species from Thailand.

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Published
2021-06-25
How to Cite
Namtaku, S., Plant, A. R., & Pramual, P. (2021). DNA Barcoding of Hemerodromia Meigen, 1822 (Diptera: Empididae) from Thailand. Tropical Natural History, 21(2), 234-243. Retrieved from https://li01.tci-thaijo.org/index.php/tnh/article/view/251305
Section
Original Articles