Palynological Studies and Lectotypification of Malpighiaceae in Thailand


  • Wanwipha Chaisongkram Program of Biology, Faculty of Science, Udon Thani Rajabhat University, Udon Thani 41000, THAILAND
  • Maneenuch Tubtamat Program of Biology, Faculty of Science, Udon Thani Rajabhat University, Udon Thani 41000, THAILAND
  • Wilaiwan Kulpholmuang Program of Biology, Faculty of Science, Udon Thani Rajabhat University, Udon Thani 41000, THAILAND
  • Pranom Chantaranothai Department of Biology and Center of Excellence on Biodiversity (BDC), Faculty of Science, Khon Kaen University, Khon Kaen 40002, THAILAND: Honorary Research Associate, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3AE, UK


exine sculpturing, lectotype, Malphigiales, pollen


The pollen morphology of 19 species belonging to six genera of the Malpighiaceae in Thailand was examined by both light- and scanning electron microscopy. Pollen grains were found to be monad and bilateral or radial symmetry. They were apolar, isopolar or heteropolar with a high range of size from very small to medium. The shapes were oblate, oblate spheroidal, prolate spheroidal and subprolate. The ambs were triangular, quadrangular, quinquangular and subcircular. Aperture types were 3−4-colporate, 3-colporate with parasyncolporate, 4-porate, polyporate, monosulcate and trichotomosulcate. Five pollen types were proposed based on the exine sculpturing viz. fossulate, granulate, perforate, rugulate and verrucate types. Lectotypes for nine taxa of Malpighiaceae were herein designated, namely Aspidopterys thorelii, Brachylophon anastomosans, Hiptage bullata, H. candicans, H. candicans var. angustifolia, H. condita, H. detergens, H. glabrifolia and H. lucida.


Anderson, W. R. 1981. Malpighiaceae, in the botany of the Guayana Highlands, part XI. Memoirs of the New York Botanical Garden, 32: 21–305.

Anderson, C. 1982. A monograph of the genus Peixotoa (Malpighiaceae). Contributions from the University of Michigan Herbarium, 15: 1–92.

Anderson, W. R. 1990. The origin of the Malpighiaceae, the evidence from morphology. Memoirs of the New York Botanical Garden, 64: 210–224.

Belonsi, T. K. and Gasparino, E. C. 2015. Pollen morphology of Malpighiaceae from Brazilian forest fragments. Brazilian Journal of Botany, 38(2): 379–393.

Cai, L., Xi, Z., Lemmon, E. M., Lemmon, A. R., Mast, A., Buddenhagen, C. E., Liu, L. and Davis, C. C. 2020. The perfect strom: gene tree estimation error, incomplete lineage sorting, and ancient gene flow explain the most recalcitrant ancient angiosperm clade, Malpighiales. Systematic Biology, 70: 491–507.

Cai, M., Zhu, H. and Wang, H. 2008. Pollen morphology of the genus Lasianthus (Rubiaceae) and related taxa from Asia. Journal of Systematics and Evolution, 46: 62–72.

Cardinal-McTeague, W. M. and Gillespie, L. J. 2016. Molecular phylogeny and pollen evolution of Euphorbiaceae Tribe Plukenetieae. Systematic Botany, 41(2): 329–347.

Cameron, K. M., Chase, M. W., Anderson, W. R. and Hills, H. G. 2001. Molecular systematics of Malpighiaceae: evidence from plastid rbcL and matK sequences. American Journal of Botany, 88(10): 1847–1862.

Celenk, S., Tarimcilar, G., Bicakci, A., Kaynak, G. and Malyer, H. 2008. A palynological study of The genus Mentha L. (Lamiaceae). Botanical Journal of the Linnean Society, 157: 141–154.

Chantaranothai, P. 1997. Palynological studies in the family Melastomataceae from Thailand. Grana, 36(3): 146–159.

Christenhusz, M. J. M., Fay, M. F. and Chase, M. W. 2017. Plant of the world: an illustrated encyclopedia of vascular plants. Royal Botanic Gardens, Kew and the University of Chicago Press, 816 pp.

Chung, K., Elisens, W. J. and Skvarla, J. J. 2010. Pollen morphology and its phylogenetic significance in tribe Sanguisorbeae (Rosaceae). Plant Systematics and Evolution, 285: 139–148.

Craib, W. G. 1926. Contributions to the flora of Siam. Additamentum XVIII. Bulletin of Miscellaneous information (Royal Botanic Gardens, Kew), 1926(4): 154–174.

Davis, C. C. and Anderson, W. R. 2010. A complete generic phylogeny of Malpighiaceae Inferred from nucleotide sequence data and morphology. American Journal of Botany, 97(12): 2031–2048.

Davis, C. C., Anderson, W. R. and Donoghue, M. J. 2001. Phylogeny of Malpighiaceae: evidence from chloroplast NADHF and TRNL-F nucleotide sequences. American Journal of Botany, 88(10): 1830–1846.

Davis, C. C., Bell, C. D., Fritsch, P.W. and Mathews, S. 2002. Phylogeny of Acridocarpus-Brachylophon (Malpighiaceae): implications for tertiary tropical floras and Afroasian biogeography. Evolution, 56: 2395–2405.

Davis, C. C., Fritsch, P.W., Bell, C. D. and Mathews, S. 2004. High latitude tertiary migrations of an exclusively tropical clade: evidence from Malpighiaceae. International Journal of Plant Sciences, 165: s107–s121.

Dop, P. L. A. 1908. Contribution a l'étude des Malpighiacées d’Indo-Chine. Bulletin de la Société botanique de France, 55: 427–439. Erdtman, G. 1952. Pollen morphology and plant taxonomy: Angiosperms. Almquist and Wiksell, Stockholm, 539 pp.

Erdtman, G. 1960. The acetolysis method: a revised description. Svensk Botanisk Tidskrift, 54: 561–564.

Erdtman, G. 1966. Pollen morphology and plant taxonomy: Angiosperms. Hafner Publishing, New York, 553 pp.

Hesse, M., Halbrintter, H., Zetter, R., Weber, M., Buchner, R., Frosch-Radivo, A. and Ulrich, L. S. 2009. Pollen terminology: an illustrated handbook. Springer, Wien, 266 pp.

Hutchinson, J. 1967. The genera of flowering plants, vol. 2. Clarendon Press, Oxford, U.K, 659 pp.

Kim, M. and Zavada, M.S. 1993. Pollen morphology of Broussonetia (Moraceae). Grana, 32: 327–329.

Makino-Watanabe, H., Melhem, T. S. and Barth, M. O. 1998. Morfologia polínica de Camarea St.-Hill.

(Malpighiaceae). Revista Brasileira de Botânica, 21: 1–6.

Marinho, L. C., Fiaschi, P., Amorim, A. M. and dos Santos, F. de A. R. 2015. The taxonomic significance of pollen morphology in Tovomita Aubl. (Clusiaceae: Clusieae) and related genera. Plant Systematics and Evolution, 301:


Mehrvarz, S. S., Yousefi, N., Mohammadi, M. and Marcussen, T. 2014. Pollen studies in the genus Viola (Violaceae) from Iran. Acta Botanica Croatica, 73(1): 93–106.

Mezzonato-Pires, A. C., Mendonça, C. B. F. and Gonçalves-Esteves, V. 2015. Pollen morphology of selected species of Passiflora L. (Passifloraceae) from the Atlantic forest. Acta Botanica Brasilica, 29(3): 391–399.

Oliveira, R. L. C., Scudeller, V. V. and Barbosa, R. I. 2017. Use and traditional knowledge of Byrsonima crassifolia and B. coccolobifolia (Malpighiaceae) in a Makuxi community of the Roraima savanna, northern Brazil. Acta Amazonica, 42(2): 133–140.

Pornpongrungrueng, P. and Chantaranothai, P. 2002. Pollen morphology of the tribe Inuleae (Compositae) in Thailand. Thai Forest Bulletin (Botany), 30: 116–123.

Punt, W., Hoen, P. P., Blackmore, S., Nilsson, S., and Le Thomas, A. 2007. Glossary of pollen and spore terminology. Review of Paleobotany and Palynology, 143: 1–81.

Rueangsawang, K., Chantaranothai, P. and Simpson, D. A. 2013. Pollen morphology of Justicia L. (Acanthaceae) from Thailand and its taxonomic value. Grana, 52(4): 275–288.

Santiago, L. J. M., Louro, R. P., Emmerich, M. and Barth, O. M. 2004. The pollen morphology of Phyllanthus (Euphorbiaceae) section Choretropsis. Botanical Journal of Linnean Society, 144(2): 243–250.

Schols, P., Furness, C. A., Wilkin, P., Smets, E., Cielen, V. and Huysmans, S. 2003. Pollen morphology of Dioscorea (Dioscoreaceae) and its relation to systematics. Botanical Journal of the Linnean Society, 143: 375–390.

Sebastiani, R., Cruz-Barros, M. A. V. and Corrêa, A. M. S. 2014. Palynological study of Janusia A. Juss. and related genera (Malpighiaceae). Brazilian Journal of Botany, 37(4): 543–559.

Shi, W., Wen, J. and Lutz, S. 2013. Pollen morphology of the Maddenia clade of Prunus and its taxonomic and phylogenetic implications. Journal of Systematics and Evolution, 51(2): 164–183.

Shukun, C. and Funston, A. M. 2008. Malpighiaceae. In: Wu, Z. Y. and Raven, P. (eds.), Flora of China, 11: 132–138. Science Press (Beijing) and Missouri Botanical Garden Press (St. Louis).

Singh, R. K. 2017. Lectotypification of the Linnaean name Banisteria benghalensis (Malpighiaceae). Journal of Japanese Botany, 92(5): 299–302.

Sirirugsa, P. 1987. Three new species of Hiptage (Malpighiaceae) from Thailand. Nordic Journal of Botany, 7(3): 277–280.

Sirirugsa, P. 1991. Malpighiaceae. In: Smitinand, T. and Larsen, K. (eds), Flora of Thailand, 5(3): 272–299. The Forest Herbarium, Royal Forest Department. Bangkok.

Souza, L. R., Carneiro-Torres, D. S., Saba, M. D. and dos Santos, F. de A. R. 2017. Pollen morphology of the Acalyphoideae and Euphorbioideae (Euphorbiaceae) of the Caatinga ecoregion in Brazil. Plant Systematics and Evolution, 303: 1161–1180.

Thammarong, W., Chantaranothai, P., Parnell, J. A. N., Hodkinson, T. R. and Pornpongrungrueng, P. 2019. Pollen morphology of Lecythidaceae in Southeast Asia. Thai Forest Bulletin (Botany), 47(1): 55–68.

Tortoriello, J. and Lozoya, X. 1992. Effect of Galphimia glauca methanolic extract on neuropharmacological tests. Planta Medica, 58: 234–236.

Welsh, M., Stefanović, S. and Costea, M. 2010. Pollen evolution and its taxonomic significance in Cuscuta (dodders, Convolvulaceae). Plant Systematics and Evolution, 285: 83–101.




How to Cite

Chaisongkram, W. , Tubtamat, M. , Kulpholmuang, W. and Chantaranothai, P. 2022. Palynological Studies and Lectotypification of Malpighiaceae in Thailand. Tropical Natural History. 22, 1 (Dec. 2022), 108–118.



Original Articles