Molecular Phylogeny Reveals a New Snorkel Snail, Rhiostoma panhai sp. nov., from Thailand (Gastropoda: Caenogastropoda: Cyclophoridae)
Keywords:
DNA barcoding, mitochondrial DNA, operculate land snail, taxonomyAbstract
The present study used two mitochondrial gene fragments (COI and 16S rRNA) and morphology to reveal a new species of Rhiostoma in Thailand: Rhiostoma panhai sp. nov. Genetic divergence between the new species and other Rhiostoma species was high, ranged from 14 to 19% p-distance. Phylogenetic analyses confirmed the novelty of this new species. The new species differs from other Rhiostoma species by having a detached whorl shorter than the aperture width (mean detached-whorl length/aperture width: 0.89±1.40), a small, curved, and slender tubular breathing device, and dark brown with paler zigzag streaks periostracum.
References
Benson, W.H. 1860. On Clostophis and Rhiostoma, new Burmese genera of land shells. Annals and Magazine of Natural History, Series 3, volume 5: 95–97.
Chen, Z.Y., Huang, B. and Páll-Gergely, B. 2022. Exceptional among the exceptional: a new species of Stenogyropsis Möllen-dorff, 1899 (Eupulmonata: Camaenidae), with a review of the free last whorl in terrestrial gastropods. Journal of Molluscan Studies, 88: eyac015.
Cole, M.L., Raheem, D.C. and Villet, M.H. 2019. Molecular phylogeny of Chondrocyclus (Gastropoda: Cyclophoridae), a widespread genus of sedentary, restricted-range snails. Mole-cular Phylogenetics and Evolution, 131: 193–210.
Dryden, I.L. and Mardia, K.V. 2016. Statistical Analysis of Shape, with Applications in R. 2nd Edition. Wiley, Chichester.
Fischer, P.H. 1973. Les mollusques testacés du Cambodge, Pre-mière partie: Introduction et Gastéropodes Prosobranches. Journal de Conchyliologie, 110: 40–64.
Fischer, P. 1891. Catalogue et distribution géographique des moll- usques terrestres, fluviatiles & marins ďune partie de ľIndo-Chine (Siam, Laos, Cambodge, Cochinchine, Annam, Tonkin). Bulletin de la Société ďHistoire Naturelle ďAutun, 4: 87–276.
Folmer, O., Black, M., Hoeh, W., Lutz, R. and Vrijenhoek, R. 1994. DNA primers for amplification of mitochondrial cyto-chrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3: 294–299.
Gude, G.K. 1921. Mollusca III, land operculates (Cyclophoridae, Truncatellidae, Assimineidae, Helicinida). In: Shipley, A.S. and Marshall, G.A.K (Eds) The Fauna of British India including Ceylon and Burma. Taylor and Francis, Red Lion Court, Fleet Street, London. 386 pp.
Habe, T. 1965. Operculated land molluscs from Southeast Asia. Nature and Life in Southeast Asia, 4: 111–127.
Hirano, T., Saito, T., von Oheimb, P.V., von Oheimb, K.C.M., Do, T.U., Yamazaki, D., Kameda, Y. and Chiba, S. 2022. Patterns of diversification of the operculate land snail genus Cyclo-phorus (Caenogastropoda: Cyclophoridae) on the Ryukyu Islands, Japan. Molecular Phylogenetics and Evolution, 169: 107407.
Kessing, B., Croom, H., Martin, A., McIntosh, C., McMillan, W.O. and Palumbi, S.P. 1989. The simple fool’s guide to PCR Version 1.0., Special publication, Honolulu, Department of Zoology, University of Hawaii.
Klingenberg, C.P. 2011. MorphoJ: an integrated software package for geometric morphometrics. Molecular Ecology Resources, 11: 353–357.
Kobelt, W. 1902. Cyclophoridae. Das Tierreich – Lieferung 16 – Mollusca. Verlag von R. Friedländer und Sohn. Berlin.
Lee, Y.C., Lue, K.Y. and Wu, W.L. 2008a. A molecular phylogenetic investigation of Cyathopoma (Prosobranchia: Cyclophoridae) in East Asia. Zoological Studies, 47: 591–604.
Lee, Y.C., Lue, K.Y. and Wu, W.L. 2008b. Molecular evidence for a polyphyletic genus Japonia (Architaenioglossa: Cyclopho-ridae) and with the description of a new genus and two new species. Zootaxa, 1792: 22–38.
Miller, M.A., Schwartz, T., Pickett, B.E., He, S., Klem, E.B., Scheuermann, R.H., Passarotti, M., Kaufman, S. and O'Leary, M.A. 2015. A RESTful API for access to phylogenetic tools via the CIPRES Science Gateway. Evolutionary Bioinformatics, 11: 43–48.
Nantarat, N., Wade, C.M., Jeratthitikul, E., Sutcharit, C. and Panha, S. 2014. Molecular evidence for cryptic speciation in the Cyclophorus fulguratus (Pfeiffer, 1854) species complex (Caenogastropoda: Cyclophoridae) with Description of New Species. PLoS ONE, 9: e109785.
Páll-Gergely, B., Naggs, F. and Asami, T. 2016. Novel shell device for gas exchange in an operculate land snail. Biology Letters, 12(7): 20160151.
Prasankok, P., Sutcharit, C., Jeratthitikul, E, Backeljau, T. and Pimvichai, P. 2023. Molecular phylogeny of the snorkel snail Rhiostoma housei, a species complex from Thailand, with descriptions of three new species. Invertebrate Systematics, 37: 211–229.
Rohlf, F.J. 2005. tpsDig, digitize landmarks and outlines, version 2.05. Department of Ecology and Evolution, State University of New York at Stony Brook.
Rohlf, F.J. 2015. The tps series of software. Hystrix, the Italian Journal of Mammalogy, 26: 1–4.
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. and Huelsen-beck, J.P. 2012. MrBayes 3.2: efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61: 539–542.
Stamatakis, A. 2006. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics, 22: 2688–2690.
Tongkerd, P., Tumpeesuwan, S., Inkhavilay, K., Prasankok, P., Jeratthitikul, E., Panha, S. and Sutcharit, C. 2023. Systematic revision of the snorkel snail genus Rhiostoma Benson, 1860 (Caenogastropoda, Cyclophoridae) with descriptions of new species. ZooKeys, 1142: 1–144.
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