Multi-locus Phylogeny Reveals a New Freshwater Mussel in the Genus Bineurus Simpson, 1900 (Unionidae: Pseudodontini) from Thailand

Authors

  • Ekgachai Jeratthitikul Animal Systematics and Molecular Ecology Laboratory, Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, THAILAND
  • Chirasak Sutcharit Animal Systematics Research Unit, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, THAILAND

Keywords:

freshwater mussels, Indochina, multi-locus phylogeny, new species, Unionida

Abstract

A new freshwater mussel species is described here as Bineurus panhai sp. nov. Morphological investigation and multi-locus phylogeny based on concatenated data of COI, 16S, and 28S gene sequences reveal that the new species is similar to the Bineurus exilis (Morelet, 1866), but it can be distinguished from the latter taxon by having a curved dorsal margin and a lower posterior end (vs. straight dorsal margin and higher posterior end). They also differ from each other by 2.32% of uncorrected COI p-distance. Bineurus panhai sp. nov. is distributed in the Bang Pakong Basin of eastern Thailand and in headwater tributaries of the Tonle Sap Basin in Thailand and Cambodia. Because of its restricted range, this new species may represent a rare endemic lineage and, hence, is a valuable target for conservation.

References

Abell, R., Thieme, M.L., Revenga, C., Bryer, M., Kottelat, M., Bogutskaya, N., Coad, B., Mandrak, N., Balderas, S.C., Buss-ing, W., Stiassny, M.L.J., Skelton, P., Allen, G.R., Unmack, P., Naseka, A., Ng, R., Sindorf, N., Robertson, J., Armijo, E., Higgins, J.V., Heibel, T.J., Wikramanayake, E., Olson, D., López, H.L., Reis, R.E., Lundberg, J.G., Sabaj Pérez, M.H. and Petry, P. 2008. Freshwater ecoregions of the World: A new map of biogeographic units for freshwater biodiversity conservation. BioScience, 58(5): 403–414.

AVMA. 2020. AVMA guidelines for the euthanasia of animals. American Veterinary Medical Association. https://www.avma. org/resources-tools/avma-policies/avma-guidelines-euthanasia-animals (accessed 12 May 2021).

Bolotov, I.N., Kondakov, A.V., Vikhrev, I.V., Aksenova, O.V., Bespalaya, Y.V., Gofarov, M.Y., Kolosova, Y.S., Konopleva, E.S., Spitsyn, V.M., Tanmuangpak, K. and Tumpeesuwan, S. 2017a. Ancient river inference explains exceptional oriental freshwater mussel radiations. Scientific Reports, 7: 2135.

Bolotov, I.N., Konopleva, E.S., Vikhrev, I.V., Gofarov, M.Y., Lopes-Lima, M., Bogan, A.E., Lunn, Z., Chan, N., Win, T., Aksenova, O.V., Tomilova, A.A., Tanmuangpak, K., Tumpee-suwan, S. and Kondakov, A.V. 2020. New freshwater mussel taxa discoveries clarify biogeographic division of Southeast Asia. Scientific Reports, 10(1): 6616.

Bolotov, I.N., Konopleva, E.S., Vikhrev, I.V., Lopes-Lima, M., Bogan, A.E., Lunn, Z., Chan, N., Win, T., Aksenova, O.V., Gofarov, M.Y., Tomilova, A.A. and Kondakov, A.V. 2019. Eight new freshwater mussels (Unionidae) from tropical Asia. Scientific Reports, 9(1): 12053.

Bolotov, I.N., Konopleva, E.S., Vikhrev, I.V., Win, T., Lunn, Z., Chan, N., Gofarov, M.Y., Kondakov, A.V., Tomilova, A.A., Pasupuleti, R. and Subba Rao, N.V. 2022. Follow the footsteps of Leonardo Fea: An example of an integrative revision of freshwater mussel taxa described from the former British Burma (Myanmar). Journal of Zoological Systematics and Evolutionary Research, 2022: 6600359.

Bolotov, I.N., Pfeiffer, J.M., Konopleva, E.S., Vikhrev, I.V., Kon-dakov, A.V., Aksenova, O.V., Gofarov, M.Y., Tumpeesuwan, S. and Win, T. 2018. A new genus and tribe of freshwater mussel (Unionidae) from Southeast Asia. Scientific Reports, 8(1): 10030.

Bolotov, I.N., Vikhrev, I.V., Kondakov, A.V., Konopleva, E.S., Gofarov, M.Y., Aksenova, O.V. and Tumpeesuwan, S. 2017b. New taxa of freshwater mussels (Unionidae) from a species-rich but overlooked evolutionary hotspot in Southeast Asia. Scientific Reports, 7: 11573.

Brandt, R.A.M. 1974. The non-marine aquatic Mollusca of Thailand. Archiv für Molluskenkunde, 105: 1–423.

Froufe, E., Bolotov, I.N., Aldridge, D.C., Bogan, A.E., Breton, S., Gan, H.M., Kovitvadhi, U., Kovitvadhi, S., Riccardi, N., Secci-Petretto, G., Sousa, R., Teixeira, A., Varandas, S., Zanatta, D., Zieritz, A., Fonseca, M.M., and Lopes-Lima, M. 2020. Meso-zoic mitogenome rearrangements and freshwater mussel (Bival-via: Unionoidea) macroevolution. Heredity, 124: 182–196.

Graf, D.L. and Cummings, K.S. 2021. A ‘big data’ approach to global freshwater mussel diversity (Bivalvia: Unionoida), with an updated checklist of genera and species. Journal of Molluscan Studies, 87(1): 034.

Graf, D.L. and Cummings, K.S. 2022. The freshwater mussels (Unionoida) of the World (and other less consequential bival-ves). Retrieved from http://mussel-project.uwsp.edu/ (accessed 7 June 2022).

Haas, F. 1920. Die Unioniden. In: Küster, H.C. (Ed.). Systema-tisches Conchylien-Cabinet von Martini und Chemnitz 9. pt. 2, h. 52, 289–304, pls. 264–268. .

Haas, F. 1969. Superfamilia Unionacea. In: Martens, R. and Hennig, W. (Eds). Das Tierrich, Lieferung. Vol. 88, Walter de Gruyter, Berlin, 1–663.

Jeratthitikul, E., Paphatmethin, S., Sutcharit, C., Ngor, P.B., Inkhavilay, K. and Prasankok, P. 2022. Phylogeny and biogeo-graphy of Indochinese freshwater mussels in the genus Pilsbry-oconcha Simpson, 1900 (Bivalvia: Unionidae) with descrip-tions of four new species. Scientific Reports, 12(1): 20458.

Jeratthitikul, E., Paphatmethin, S., Zieritz, A., Lopes-Lima, M. and Ngor, P.B. 2021a. Hyriopsis panhai, a new species of freshwater mussel from Thailand (Bivalvia: Unionidae). Raffles Bulletin of Zoology, 69: 124–136.

Jeratthitikul, E., Phuangphong, S., Sutcharit, C., Prasankok, P., Kon-gim, B. and Panha, S. 2019a. Integrative taxonomy reveals phe-notypic plasticity in the freshwater mussel Contradens contra-dens (Bivalvia: Unionidae) in Thailand, with a descrip-tion of a new species. Systematics and Biodiversity, 17(2): 134–147.

Jeratthitikul, E., Sutcharit, C. and Prasankok, P. 2019b. Molecular phylogeny of the Indochinese freshwater mussel genus Scabies Haas, 1911 (Bivalvia: Unionidae). Tropical Natural History, 19: 21–36.

Jeratthitikul, E., Sutcharit, C., Ngor, P.B. and Prasankok, P. 2021b. Molecular phylogeny reveals a new genus of freshwater mussels from the Mekong River Basin (Bivalvia: Unionidae). European Journal of Taxonomy 775: 119–142.

Kongim, B., Sutcharit, C. and Panha, S. 2015. Cytotaxonomy of unionid freshwater mussels (Unionoida, Unionidae) from northeastern Thailand with description of a new species. ZooKeys, 514: 93–110.

Konopleva, E.S., Bolotov, I.N., Kondakov, A.V., Kononov, O.D., Gofarov, M.Y., Tomilova, A.A., Lunn, Z., Chan, N., Win, T. and Vikhrev, I.V. 2020. A taxonomic review of Trapezidens (Bivalvia: Unionidae: Lamellidentini), a freshwater mussel genus endemic to Myanmar, with a description of a new species. Ecologica Montenegrina, 27: 45–57.

Konopleva, E.S., Bolotov, I.N., Pfeiffer, J.M., Vikhrev, I.V., Kon-dakov, A.V., Gofarov, M.Y., Tomilova, A.A., Tanmuangpak, K. and Tumpeesuwan, S. 2021. New freshwater mussels from two Southeast Asian genera Bineurus and Thaiconcha (Pseudodon-tini, Gonideinae, Unionidae). Scientific Reports, 11(1): 8244.

Konopleva, E.S., Bolotov, I.N., Spitsyn, V.M., Kondakov, A.V., Gofarov, M.Y. and Vikhrev, I.V. 2019a. A new Contradens from Laos (Bivalvia: Unionidae: Contradentini). Ecologica Montenegrina, 24: 25–31.

Konopleva, E.S., Lheknim, V., Sriwoon, R., Kondakov, A.V., Tomilova, A.A., Gofarov, M.Y., Vikhrev, I.V., Bolotov, I.N. 2023. Diversity and phylogenetics of freshwater mussels (Unionidae) from Southern Thailand with the description of one new genus and five new species-group taxa. Diversity, 15: 10.

Konopleva, E.S., Pfeiffer, J.M., Vikhrev, I.V., Kondakov, A.V., Gofarov, M.Y., Aksenova, O.V., Lunn, Z., Chan, N. and Bolotov, I.N. 2019b. A new genus and two new species of freshwater mussels (Unionidae) from western Indochina. Scientific Reports, 9(1): 4106.

Kumar, S., Stecher, G. and Tamura, K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7): 1870–1874.

Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. and Calcott, B. 2016. PartitionFinder 2: New methods for selecting parti-tioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution, 34(3): 772–773.

Lea, I. 1863. Descriptions of eleven new species of exotic Unionidae. Proceedings of the Academy of Natural Sciences of Philadelphia 15: 189–190.

Miller, M.A., Pfeiffer, W. and Schwartz, T. 2010. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. 2010 Gateway Computing Environments Workshop (GCE): 1–8.

Morelet, A. 1875. Séries Conchyliologiques: Comprenant l’énumé-ration de Mollusques terrestres et fluviatiles recueilles pendant le cours de différents voyages ainsi que la description de plusieurs espèces nouvelles 4, Chez Klincksieck, Paris.

Muanta, S., Jeratthitikul, E., Panha, S. and Prasankok, P. 2019. Phy-logeography of the freshwater bivalve genus Ensidens (Union-idae) in Thailand. Journal of Molluscan Studies, 85(2): 224–231.

Ng, T.H., Jeratthitikul, E., Sutcharit, C., Chhuoy, S., Pin, K., Pholyo-tha, A., Siriwut, W., Srisonchai, R., Hogan, Z.S. and Ngor, P.B. 2020. Annotated checklist of freshwater molluscs from the larg-est freshwater lake in Southeast Asia. ZooKeys, 958: 107–141.

Pfeiffer, J.M. and Graf, D.L. 2015. Evolution of bilaterally asymmetrical larvae in freshwater mussels (Bivalvia: Unio-noida: Unionidae). Zoological Journal of the Linnean Society, 175 (2), 307–318.

Pfeiffer, J.M., Graf, D.L., Cummings, K.S. and Page, L.M. 2021. Taxonomic revision of a radiation of Southeast Asian fresh-water mussels (Unionidae: Gonideinae: Contradentini+Recti-dentini). Invertebrate Systematics, 35(4): 394–470.

Prié, V. and Puillandre, N. 2014. Molecular phylogeny, taxonomy, and distribution of French Unio species (Bivalvia, Unionidae). Hydrobiologia, 735(1): 95–110.

Rainboth, W.J., Vidthayanon, C. and Yen, M.D. 2012. Fishes of the greater Mekong ecosystem with species list and photographic atlas. Miscellaneous Publications of the Museum of Zoology, University of Michigan, 201: 1–294.

Rochebrune, A. 1882. Documents sur la faune malacologique de la Cochinchine et du Cambodge. Bulletin de la Société Philomathique de Paris, 7(1): 35–74.

Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. and Huelsen-beck, J.P. 2012. MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61(3): 539–542.

Simpson, C.T. 1900. Synopsis of the naiades, or pearly fresh-water mussels. Proceedings of the United States National Museum, 22: 501–1044.

Simpson, C.T. 1914. A descriptive catalogue of the naiades, or pearly fresh-water mussels. Parts I–III., Bryant Walker, Detroit, Michigan., 1540 pp.

Smith, C.H., Johnson, N.A., Inoue, K., Doyle, R.D. and Randklev, C.R. 2019. Integrative taxonomy reveals a new species of freshwater mussel, Potamilus streckersoni sp. nov. (Bivalvia: Unionidae): implications for conservation and management. Systematics and Biodiversity, 17(4): 331–348.

Stamatakis, A. 2014. RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30(9): 1312–1313.

Voris, H.K. 2000. Maps of Pleistocene sea levels in Southeast Asia: shorelines, river systems and time durations. Journal of Biogeography, 27(5): 1153–1167.

Zieritz, A., Lopes-Lima, M., Bogan, A.E., Sousa, R., Walton, S., Rahim, K.A., Wilson, J.J., Ng, P.Y., Froufe, E. and McGowan, S. 2016. Factors driving changes in freshwater mussel (Bivalvia, Unionida) diversity and distribution in Peninsular Malaysia. Science of The Total Environment, 15: 1069–1078.

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Published

2023-05-06

How to Cite

[1]
Jeratthitikul, E. and Sutcharit, C. 2023. Multi-locus Phylogeny Reveals a New Freshwater Mussel in the Genus Bineurus Simpson, 1900 (Unionidae: Pseudodontini) from Thailand. Tropical Natural History. 7 (May 2023), 173–180.