Effects of Pediastrum boryanum and Dried Chlorella as Feeds on the Growth Performance and Carotenoid Content of the Fairy Shrimp Branchinella thailandensis (Branchiopoda, Anostraca)


  • Sutthana Plodsomboon Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani 34190, THAILAND
  • Laorsri Sanoamuang International College, Khon Kaen University, Khon Kaen 40002, THAILAND: Applied Taxonomic Research Center, Faculty of Science, Khon Kaen University, Khon Kaen 40002, THAILAND


aquaculture, carotenoid, Chlorella vulgaris, freshwater, microalgal diets, survival rate


In this study, fresh Pediastrum boryanum and dried chlorella were chosen as alternative diets to feed the fairy shrimp Branchinella thailandensis instead of its most common, Chlorella vulgaris. In three separate trials, 5-day-old B. thailandensis was fed 6.8 x 106 cells mL-1 of fresh C. vulgaris (as a control), 6.8 x 106 cells mL-1 of fresh P. boryanum, and 3 mg of dry weight per individual of powdered chlorella for 15 days. Animals in each experiment were fed twice a day for 15 days with three replicates (n = 100 individuals per replicate). The results showed that the body length of B. thailandensis, which was fed fresh P. boryanum and dried chlorella for 5 days, had increased more than the control. However, at the end of the experiment, B. thailandensis fed with dried chlorella had a longer body length than those fed the control or P. boryanum (P > 0.05). The fairy shrimp fed with P. boryanum for 15 days had the highest survival rate of 70.8%, followed by the control (70.0%) and dried chlorella (28.3%) (P < 0.05). Additionally, B. thailandensis fed with P. boryanum had the highest protein content of 73.37%, compared to those fed with dried chlorella (63.77%) and the control (54.14%) (P < 0.05). In contrast, the treatments fed with P. boryanum had significantly lower lipid and carbohydrate contents (3.21 and 11.29%) than those fed with the control (3.56, 26.88%) and the dried chlorella (3.50, 26.53%) (P < 0.05). The highest total carotenoid content, however, was 380.19 g g-1 in B. thailandensis fed with dried chlorella, followed by P. boryanum (310.91 g g-1) and the control (200.53 g g-1 dry weight) (P < 0.05). Therefore, it is possible to promote fresh P. boryanum as a substitute for feeding fairy shrimp. Nonetheless, it is advised to feed the shrimp that were reared for 5–10 days with dried chlorella when live algae are scarce. These results will aid the development of shrimp farming practices.


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How to Cite

Plodsomboon, S. and Sanoamuang, L. 2023. Effects of Pediastrum boryanum and Dried Chlorella as Feeds on the Growth Performance and Carotenoid Content of the Fairy Shrimp Branchinella thailandensis (Branchiopoda, Anostraca). Tropical Natural History. 23, 1 (Jul. 2023), 73–81.



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