A New Thai Millipede Species of the Genus Coxobolellus Pimvichai, Enghoff, Panha & Backeljau, 2020 (Diplopoda: Spirobolida: Pseudospirobolellidae)
DOI:
https://doi.org/10.58837/tnh.25.8.266861Keywords:
DNA barcoding, gonopod morphology, identification key, phylogenyAbstract
A new millipede species of the genus Coxobolellus is described from Mae Hong Son Province, Thailand: Coxobolellus sirin-dhornae sp. nov. The new species is defined by its gonopod morphology and COI DNA barcodes. These latter place the new species within the well-supported Coxobolellus clade. The new species exhibits four gonopod synapomorphies that are diagnostic for the genus: (1) a protruding process on the coxae of the 3rd (and sometimes 4th) pair of male legs, (2) large, triangular coxae on the 4th and 5th pairs of legs, (3) a short process on the preanal ring extending to or slightly beyond the anal valves, and (4) the posterior gonopod telopodite divided into two parts, with a conspicuous pore opening at the mesal margin at the end of the coxal part. The new species is included in an updated morphological identification key for all currently recognized Coxobolellus species.
References
Darriba, D., Taboada, G.L., Doallo, R. and Posada, D. 2012. jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9: 772. https://doi.org/10.1038/ nmeth.2109.
Edgar, R.C. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32: 1792–1797. https://doi.org/10.1093/nar/gkh340
Enghoff, H., Golovatch, S., Short, M., Stoev, P. and Wesener, T. 2015. Diplopoda – Taxonomic overview. In ‘The Myriapoda 2. Treatise on Zoology – Anatomy, Taxonomy, Biology’. (Ed A Minelli) pp. 363–453. (Brill: Leiden, Netherlands)
Folmer, O., Black, M., Hoeh, W., Lutz, R. and Vrijenhoek, R. 1994. DNA primers for amplification of mitochondrial cytoch-rome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3: 294–299.
Hillis, D. and Bull, J. 1993. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology, 42: 182–192. https://doi.org/10.1093/ sysbio/42.2.182
Huelsenbeck, J.P. and Ronquist, F. 2001. MRBAYES: Bayesian inference of phylogeny. Bioinformatics, 17: 754–755. https://doi.org/10.1093/bioinformatics/17.8.754
Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35, 1547–1549. https://doi:10.1093/molbev/msy096
Miller, M.A., Pfeiffer, W. and Schwartz, T. 2010. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In ‘Proceedings of the Gateway Computing Environments Workshop (GCE)’, 14 November 2010, New Orleans, LA, USA. INSPEC, Accession Number: 11705685, pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129
Pimvichai, P., Enghoff, H., Panha, S. and Backeljau, T. 2018. Morphological and mitochondrial DNA data reshuffle the taxonomy of the genera Atopochetus Attems, Litostrophus Chamberlin and Tonkinbolus Verhoeff (Diplopoda: Spirobo-lida: Pachybolidae), with descriptions of nine new species. Invertebrate Systematics, 32: 159–195. https://doi.org/10.1071/ IS17052
Pimvichai, P., Enghoff, H., Panha, S. and Backeljau, T. 2020. Integrative taxonomy of the new millipede genus Coxobolellus, gen. nov. (Diplopoda: Spirobolida: Pseudospirobolellidae), with descriptions of ten new species. Invertebrate Systematics, 34: 591–617. https://doi.org/10.1071/IS20031
Pimvichai, P., Enghoff, H. and Backeljau, T. 2022a. Two new millipede species of the genus Coxobolellus Pimvichai, Enghoff, Panha & Backeljau, 2020 (Diplopoda, Spirobolida, Pseudospirobolellidae. ZooKeys, 1128: 171–190. https://doi. org/ 10.3897/zookeys.1128.94242
Pimvichai, P., Enghoff, H., Panha, S. and Backeljau, T. 2022b. A new genus of Pseudospirobolellidae (Diplopoda, Spirobolida) from limestone karst areas in Thailand, with descriptions of three new species. Zoosystematics and Evolution, 98(2): 313–326. https://doi.org/10.3897/zse.98.90032
San Mauro, D. and Agorreta, A. 2010. Molecular systematics: A synthesis of the common methods and the state of knowledge. Cellular & Molecular Biology Letters, 15: 311–341. https://doi. org/10.2478/s11658-010-0010-8
Stamatakis, A. 2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30: 1312–1313. https://doi.org/10.1093/ bioinformatics/btu033
Xia, X. 2018. DAMBE7: new and improved tools for data analysis in molecular biology and evolution. Molecular Biology and Evolution, 35: 1550–1552. https://doi.org/10.1093/molbev/ msy073
Downloads
Published
How to Cite
License
Chulalongkorn University. All rights reserved. No part of this publication may be reproduced, translated, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, without prior written permission of the publisher




