Discovery of a New Helicarionoidean Land Snail Genus Siamopotaxis gen. nov. (Eupulmonata: Helicarionidae) from Thailand Based on Molecular Phylogenetic and Morphological Evidence
DOI:
https://doi.org/10.58837/tnh.25.8.267115Keywords:
Diversity, Endemic, Indochina, Land snail, Limestone, TaxonomyAbstract
The new helicarionoidean land snail genus Siamopotaxis Pholyotha, gen. nov. is described for two new species, Siamopotaxis principalis Pholyotha, sp. nov. and Siamopotaxis thailandensis Pholyotha, sp. nov., both from Thailand. The new genus is characterised by a medium-sized, globose, high-spired, cream-coloured shell, with one or two supraperipheral bands, a rounded last whorl, a short epiphallic caecum, an elongated dart apparatus, and many radular teeth with pectiniform cusps. Siamopotaxis principalis sp. nov. differs from S. thailandensis sp. nov. by the presence of one spiral band, whereas the latter species has two spiral bands. A DNA sequence analysis of mitochondrial COI and 16S DNA, and nuclear 28S DNA gene fragments, supports the systematic placement of these new taxa and confirms that Siamopotaxis gen. nov. as a distinct clade within the Helicarionidae.
References
American Veterinary Medical Association. 2020. AVMA Guide-lines for the euthanasia of animals: 2020 Edition. https://www. avma.org/sites/default/files/2020-01/2020-Eutha nasia-Final-1-17-20.pdf [accessed 12 Feb 2020]
Bhosale, A.R., Saadi, A.J., Wade, C.M., Thackeray, T.U., Tamboli, A.S., Kadam, S.K., Muley, D.V. and Raheem, D.C. 2021. Varadia, a new helicarionoidean semi-slug genus from India’s Western Ghats (Stylommatophora: Helicarionoidea). European Journal of Taxonomy, 757: 50–79. https://doi.org/10.5852/ejt. 2021.757.1413
Blanford, W.T. and Godwin-Austen, H.H. 1908. Mollusca: Testa-cellidae and Zonitidae. In: Bingham C.T. (ed.) The Fauna of British India including Ceylon and Burma. Taylor and Francis, London.
Chang, Z.-Y. and Liew T.-S. 2021. A molecular phylogeny of Geotrochus and Trochomorpha species (Gastropoda: Trocho-morphidae) in Sabah, Malaysia reveals convergent evolution of shell morphology driven by environmental influences. PeerJ, 9: e10526. https://doi.org/10.7717/peerj.10526
Clements, R., Sodhi, N.S., Schilthuizen, M., and Ng, P.K.L. 2006. Limestone karsts of Southeast Asia: Imperiled arks of biodi-versity. Bioscience, 56(9): 733–742. https://doi.org/10.1641/ 0006-3568(2006)56[733:lkosai]2.0.co;2
Clements, R., Ng, P.K.L., Lu, X.X., Ambu, S., Schilthuizen, M. and Bradshaw, C.J.A. 2008. Using biogeographical patterns of endemic land snails to improve conservation planning for limestone karsts. Biological Conservation, 141(11): 2751–2764. https://doi.org/10.1016/j.biocon.2008.08.011
Criscione, F. and Köhler, F. 2013. Conserved shell disguises diversity in Mesodontrachia land snails from the Australian Monsoon Tropics (Gastropoda: Camaenidae). Zoologica Scripta, 42: 389–405. https://doi.org/10.1111/zsc.12011
Criscione, F., and Köhler, F. 2014. Cardiotrachia and Rachita – two new land snail genera from the East Kimberley, Western Australia (Eupulmonata: Camaenidae). Systematics and Biodi-versity, 12(1): 55–64. https://doi.org/10.1080/14772000.2013. 877998
Criscione, F., Law, M.L. and Köhler, F. 2012. Land snail diversity in the monsoon tropics of Northern Australia: revision of the genus Exiligada Iredale, 1939 (Mollusca: Pulmonata: Camaenidae), with description of 13 new species. Zoological Journal of the Linnean Society, 166: 689–722. https://doi.org/ 10.1111/j.1096-3642.2012.00863.x
Felsenstein, J. 2004. Inferring phylogenies. Sinauer Associates, Massachusetts, Sunderland.
Folmer, O., Black, M., Hoeh, W., Lutz, R. and Vrijenhoek, R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3: 294–299.
Foon, J.K., Clements, G.R. and Liew, T.-S. 2017. Diversity and biogeography of land snails (Mollusca, Gastropoda) in the limestone hills of Perak, Peninsular Malaysia. ZooKeys, 682: 1–94. https://doi.org/10.3897/zookeys.682.12999
Godwin-Austen, H.H. 1882–1920. Land and freshwater Mollusca of India, including South Arabia, Baluchistan, Afghanistan, Kashmir, Nepal, Burmah, Pegu, Tenasserim, Malay Peninsula, Ceylon, and other islands of the Indian Ocean. Supplementary to Messrs. Theobald and Hanley's Conchologia Indica. Taylor and Francis, London.
Grismer, L.L., Wood, P.L. Jr, Thura, M.K., Quah, E.S.H., Murdoch, M.L., Grismer, M.S., Herr, M.W., Lin, A. and Kyaw, H. 2018a. Three more new species of Cyrtodactylus (Squamata: Gekkonidae) from the Salween Basin of eastern Myanmar underscore the urgent need for the conservation of karst habitats. Journal of Natural History, 52: 1243–1294. https://doi. org/10.1080/00222933.2018.1449911
Grismer, L.L., Wood, Jr, P.L., Thura, M.K., Zin, T., Quah, E.S.H., Murdoch, M.L., Grismer, M.S., Lin, A., Kyaw, H. and Lwin, N. 2018b. Twelve new species of Cyrtodactylus Gray (Squamata: Gekkonidae) from isolated limestone habitats in east-central and southern Myanmar demonstrate high localized diversity and unprecedented microendemism. Zoological Journal of the Linnean Society, 182: 862–959. https://doi.org/10.1093/zoolin nean/zlx057
Gupta, A. 2005. Landforms of Southeast Asia. In: Gupta, A. (Ed.). The physical geography of Southeast Asia. New York: Oxford University Press, 38–64.
Hemmen, J. and Hemmen, C. 2001. Aktualisierte Liste der terres-trischen Gastropoden Thailands. Schriften zur Malakozoologie, 18: 35–70.
Hillis, D.M. and Bull, J.J. 1993. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology, 42: 182–192. https://doi.org/10.1093/ sysbio/ 42.2.182
Huelsenbeck, J.P. and Rannala, B. 2004. Frequentist properties of Bayesian posterior probabilities of phylogenetic trees under simple and complex substitution models. Systematic Biology, 53: 904–913. https://doi.org/10.1080/10635150490522629
Inkhavilay, K., Sutcharit, C., Bantaowong, U., Chanabun, R., Siriwut, W., Srisonchai, R., Pholyotha, A., Jirapatrasilp, P. and Panha, S. 2019. Annotated checklist of the terrestrial molluscs from Laos (Mollusca, Gastropoda). ZooKeys, 834: 1–166. https://doi.org/10.3897/zookeys.834.28800
Jirapatrasilp, P., Tongkerd, P., Jeratthitikul, E., Liew, T.-S., Pholyotha, A., Sutcharit, C. and Panha, S. 2021a. Molecular phylogeny of the limacoid snail family Dyakiidae in Southeast Asia, with the description of a new genus and species. Zoological Journal of the Linnean Society, 193: 250–280. https://doi.org/10.1093/zoolinnean/zlaa129
Jirapatrasilp, P., Páll-Gergely, B., Sutcharit, C. and Tongkerd, P. 2021b. The operculate micro land snail genus Dicharax Kobelt & Möllendorff, 1900 (Caenogastropoda, Alycaeidae) in Thailand, with description of new species. Zoosystematics and Evolution, 97(1): 1–20. https://doi.org/10.3897/zse.97.59143
Katoh, K., Rozewicki, J. and Yamada, K.D. 2017. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics, 20: 1160–1166.
Kumar, S., Stecher, G. and Tamura, K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33: 1870–1874.
Mauro, D.S. and Agorreta, A. 2010. Molecular systematics: a synthesis of the common methods and the state of knowledge. Cellular and Molecular Biology Letters, 15: 311–341. https://doi.org/10.2478/s11658-010-0010-8
Miller, M.A., Pfeiffer, W. and Schwartz, T. 2010. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop (GCE), 14 Nov. 2010, New Orleans, LA, 1–8.
Naggs, F., Panha, S. and Raheem, D. 2006. Developing land snail expertise in South and Southeast Asia, a new Darwin Initiative Project. The Natural History Journal of Chulalongkorn University, 6: 43–46.
Páll-Gergely, B., Fehér, Z., Otani, J.U. and Asami, T. 2017. An integrative taxonomic approach to infer the systematic position of Chalepotaxis Ancey, 1887 (Gastropoda: Stylommatophora: Helicarionidae). Molluscan Research, 37: 113–119. https://doi. org/ 10.1080/13235818.2016.1234996
Palumbi, S., Martin, A., Romano, S., McMillan, W.O., Stice, L. and Grabowwski, G. 1991. The simple fool’s guide to PCR. Department of Zoology, University of Hawaii, Honolulu.
Panha, S. 1996. A checklist and classification of the terrestrial pulmonate snails of Thailand. Walkerana, 8: 31–40.
Pholyotha, A., Sutcharit, C. and Panha, S. 2018. The land snail genus Macrochlamys Gray, 1847 from Thailand, with descrip-tions of five new species (Pulmonata: Ariophantidae). Raffles Bulletin of Zoology, 66: 763–781.
Pholyotha, A., Sutcharit, C., Tongkerd, P. and Panha, S. 2020. Integrative taxonomic revision of the land snail genus Sarika Godwin-Austen, 1907 in Thailand, with descriptions of nine new species (Eupulmonata, Ariophantidae). ZooKeys, 976: 1–100. https://doi.org/10.3897/zookeys.976.53859
Pholyotha, A., Sutcharit, C., Tongkerd, P. and Panha, S. 2021a. Systematic revision of the limestone karst-restricted land snail genus Aenigmatoconcha (Eupulmonata: Helicarionidae), with description of a new species. European Journal of Taxonomy, 767: 55–82. https://doi.org/10.5852/ejt.2021.767.1487
Pholyotha, A., Sutcharit, C., Tongkerd, P., Jeratthitikul, E. and Panha, S. 2021b. Integrative systematics reveals the new land-snail genus Taphrenalla (Eupulmonata: Ariophantidae) with a description of nine new species from Thailand. Contributions to Zoology, 90: 21–69. https://doi.org/10.1163/18759866-bja 10013
Pholyotha, A., Sutcharit, C. and Panha, S. 2021c. Rediscovering the dancing semislug genus Cryptosemelus Collinge, 1902 (Eupulmonata, Ariophantidae) from Thailand with description of two new species. ZooKeys, 1076: 43–65. https://doi.org/10. 3897/zookeys.1076.75576
Pholyotha, A., Sutcharit, C., Jirapatrasilp, P., Ngor, P.B., Oba, Y. and Panha, S. 2021d. Molecular phylogenetic and morphological evidence reveal a rare limacoid snail genus, Khmerquantula gen. nov. (Eupulmonata: Dyakiidae) from Cambodia. Systematics and Biodiversity, 19(8): 1049–1061. https://doi.org/10.1080/14772000.2021.1970045
Pholyotha, A., Sutcharit, C., Lin, A. and Panha, S. 2022a. Uncover-ing local endemism from southeastern Myanmar: description of the new karst-associated terrestrial snail genus Burmochlamys (Eupulmonata, Helicarionidae). ZooKeys, 1110: 1–37. https:// doi.org/10.3897/zookeys.1110.82461
Pholyotha, A., Sutcharit, C., Lin, A. and Panha, S. 2022b. Multi-gene phylogeny reveals the ribbed shell morphotypes in the land snail genus Sarika (Eupulmonata: Ariophantidae), with description of two new species from Thailand and Myanmar. Contributions to Zoology, 91: 97–132. https://doi.org/10.1163/ 18759866-bja10027
Pholyotha, A., Panha, S., Sutcharit, C., Jirapatrasilp, P., Seesamut, T., Liew, T.-S. and Tongkerd, P. 2023a. Molecular phylogeny of the land snail family Euconulidae in Thailand and its position in the superfamily Trochomorphoidea (Stylomma-tophora: Limacoidei), with description of a new genus. Invertebrate Systematics, 37(8): 571–605. https://doi.org/10. 1071/is23012
Pholyotha, A., Tongkerd, P. and Sutcharit, C. 2023b. Siamochlamys panhai, a new limestone karst‐restricted terrestrial snail from Thailand (Eupulmonata: Helicarionidae). Tropical Natural History, Supplement 7: 41–50.
Pholyotha, A., Sutcharit, C. and Panha, S. 2023c. Taxonomic revision of the land-snail genus Holkeion Godwin-Austen, 1908 (Eupulmonata: Ariophantidae), with a description of a new species, H. nordsiecki. Archiv für Molluskenkunde, 152(2): 269–280. https://doi.org/10.1127/arch.moll/152/269-280
Pholyotha, A., Sutcharit, C., Panha, S. and Tongkerd, P. 2024a. Reassessment and phylogenetic position of the overlooked limacoid land snail Trochomorpha sculpticarina Martens, 1883 (Eupulmonata, Ariophantidae), with the description of a new genus. Zoosystematics and Evolution, 100(3): 1135–1154. https://doi.org/10.3897/zse.100.129455
Pholyotha, A., Sutcharit, C., Lwin, N., Panha, S. 2024b. Unveiling the diversity of the semislug genus Durgella Blanford, 1863 (Eupulmonata: Helicarionidae) from Thailand and Myanmar, with description of two new species. Zoological Studies, 63: 14. http://10.6620/ZS.2024.63-14
Preece, R.C., White, T.S., Raheem, D.C., Ketchum, H., Ablett, J., Taylor, H., Webb, K. and Naggs, F. 2022. William Benson and the golden age of malacology in British India: Biography, illustrated catalogue and evaluation of his molluscan types. Tropical Natural History, Supplement 6: 1–434.
Ridd, M.F., Barber, A.J. and Crow, M.J. 2011. Introduction to the geology of Thailand. In: Ridd MF, Barber AJ, Crow MJ (Eds). The Geology of Thailand. Geological Society, London.
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. and Huelsenbeck, J.P. 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61: 539–542.
Saadi, A.J. and Wade, C.M. 2019. Resolving the basal divisions in the stylommatophoran land snails and slugs with special emphasis on the position of the Scolodontidae. Molecular Phylogenetics and Evolution, 139: 106529. https://doi.org/10. 1016/j.ympev.2019.106529
Schileyko, A.A. 2002. Treatise on recent terrestrial pulmonated mollusks. Part 9. Helicarionidae, Gymnarionidae, Rhysotinidae, Ariophantidae. Ruthenica, supplement 2: 1167–1307.
Schileyko, A.A. 2003. Treatise on recent terrestrial pulmonate molluscs. Part 10. Ariophantidae, Ostracolethidae, Ryssotidae, Milacidae, Dyakiidae, Staffordiidae, Gastrodontidae, Zonitidae, Daudebardiidae, Parmacellidae. Ruthenica, supplement 2: 1309–1466.
Solem, A. 1966. Some non-marine mollusks from Thailand. Spolia Zoologia Musei Hauniensis, 24: 1–110.
Stamatakis, A. 2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics (Oxford, England), 30: 1312–1313.
Sutcharit, C., Jeratthitikul, E., Pholyotha, A., Lin, A. and Panha, S. 2020a. Molecular phylogeny reveals high diversity and endemism in the limestone karst-restricted land snail genus Sophina Benson, 1859 from Myanmar (Eupulmonata: Helicari-onidae), with description of four new species. Journal of Zoological Systematics and Evolutionary Research, 58: 957–981. https://doi.org/10.1111/jzs.12420
Sutcharit, C., Thach, P., Chhuoy, S., Ngor, P.B., Jeratthitikul, E., Siriwut, W., Srisonchai, R., Ng, T.H., Pholyotha, A., Jirapatrasilp, P. and Panha, S. 2020b. Annotated checklist of the land snail fauna from southern Cambodia (Mollusca, Gastropoda). ZooKeys, 948: 1–46. https://doi.org/10.3897/ zookeys.948.51671
Sutcharit, C., Jeratthitikul, E., Tongkerd, P. and Panha, S. 2021. Reassessment and systematic position of the sinistral snails of genus Hemiplecta from Thailand (Eupulmonata: Ariophan-tidae), with description of two new species. Contributions to Zoology, 90: 183–215. https://doi.org/10.1163/18759866-BJA 10016
Sutcharit, C. and Pholyotha, A. 2023. Hidden lineages in the mountains: The genus Glyptaulax Gude, 1914 and Maela-maodiscus gen. nov. (Heterobranchia: Stylommatophora: Charopidae and Ariophantidae) with description of two new species from Western Thailand. Tropical Natural History, Supplement 7: 123–138.
Tanabe, A.S. 2011. Kakusan4 and Aminosan: two programs for comparing nonpartitioned, proportional and separate models for combined molecular phylogenetic analyses of multilocus sequence data. Molecular Ecology Resources, 11: 914–921.
Vermeulen, J.J. and Liew, T.-S. 2022. Land snails and slugs of Sabah and Labuan (Malaysia). Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia. 432 pp.
Wade, C.M., Mordan, P.B. and Clarke, B. 2001. A phylogeny of the land snails (Gastropoda: Pulmonata). Proceedings of the Royal Society of London. Series B, Biological Sciences, 268(1465): 413–422. https://doi.org/10.1098/rspb.2000.1372
Wade, C.M., Mordan, P.B. and Naggs, F. 2006. Evolutionary relationships among the pulmonate land snails and slugs (Pulmonata, Stylommatophora). Biological Journal of the Linnean Society, 87: 593–610. https://doi.org/10.1111/j.1095-8312.2006.00596.x
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