Three Novel Ascomycetous Yeast Species Isolated from Plant Leaves and Wild Mushrooms Collected in Thailand

Authors

  • Pannida Khunnamwong Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, THAILAND: Biodiversity Center Kasetsart University (BDCKU), Bangkok 10900, THAILAND
  • Pajongwan Gungprakhon Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, THAILAND
  • Panadda Nualthaisong Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, THAILAND
  • Baramee Sakolrak Department of National Parks, Wildlife and Plant Conservation, Bangkok 10900, THAILAND
  • Nanata Srisuk Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, THAILAND: Biodiversity Center Kasetsart University (BDCKU), Bangkok 10900, THAILAND
  • Savitree Limtong Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, THAILAND: Biodiversity Center Kasetsart University (BDCKU), Bangkok 10900, THAILAND: Academy of Science, The Royal Society of Thailand, Bangkok 10210, THAILAND

DOI:

https://doi.org/10.58837/tnh.25.8.267392

Keywords:

Blastobotrys princeps, Blastobotrys siamensis, Cyberlindnera sirindhorniae, Mangrove Forest, Mushroom

Abstract

Four yeast strains representing three novel species were isolated from two sources in Thailand, one from the leaf surface of a tall-stilt mangrove (Rhizophora apiculata, strain DMKU-RG45) and three from the fruiting bodies of wild mushrooms (strains DMKU-TM03, DMKU-SM11, and DMKU-SM28) collected in Thailand. Sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region revealed that these four strains differed by 0.87–8.68% and 2.07–13.55% in their nucleotide divergence from their closest described species in the sequences of the D1/D2 domain of the LSU rRNA gene and the ITS region, respectively. In phylogenetic analyses, the strain DMKU-RG45 belonged to the genus Cyberlindnera and was distinct from other recognized species in this genus, whereas the strains DMKU-TM03, DMKU-SM11, and DMKU-SM28 were members of the genus Blastobotrys. However, they were in two different positions, each of which was distinct from closely related species. Based on molecular analyses and phenotypic characteristics, the strain DMKU-RG45 is proposed as Cyberlindnera sirindhorniae f.a., sp. nov. (the holotype is TBRC 19863T; MycoBank No. is MB 858763). The other three strains (DMKU-TM03, DMKU-SM11, and DMKU-SM28) are proposed as two novel Blasto-botrys species. Blastobotrys princeps f.a., sp. nov. is proposed to accommodate the strain DMKU-TM03 (the holotype is TBRC 19382T; MycoBank No. is MB 858764). Blastobotrys siamensis f.a., sp. nov. is proposed to accommodate both strains DMKU-SM11 and DMKU-SM28 (the holotype is TBRC 19380T; MycoBank No. is MB 858765).

References

Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D.J. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic acids research, 25(17): 3389–3402.

Angchuan, J., Khunnamwong, P., Wongpanit, K., Limtong, S. and Srisuk, N. 2021. Yeasts associated with the small-intestinal contents and epithelium of Pon Yang Kham (Charolais cross-bred) fattening beef cattle. Microorganisms, 9(7): 1444.

Barretto, D.A., Avchar, R., Carvalho, C., Sampaio, J.P., Vootla, S.K. and Baghela, A. 2018. Blastobotrys bombycis sp. nov., a d-xylose-fermenting yeast isolated from the gut of the silkworm larva Bombyx mori. International Journal of Systematic and Evolutionary Microbiology, 68(8): 2638–2643.

Barros, K.O., Souza, R.M., Palladino, F., Cadete, R.M., Santos, A.R.O., Goes-Neto, A., Berkov, A., Zilli, J.E., Vital, M.J., Lac-hance, M.A. and Rosa, C.A. 2021. Cyberlindnera dasilvae sp. nov., a xylitol-producing yeast species isolated from rotting wood and frass of cerambycid larva. International Journal of Systematic and Evolutionary Microbiology, 71(9): 004986.

Boonmak, C., Khunnamwong, P. and Limtong, S. 2020. Yeast communities of primary and secondary peat swamp forests in southern Thailand. Antonie Van Leeuwenhoek, 113(1): 55–69.

Boontham, W., Limtong, S., Rosa, C.A., Lopes, M.R., Vital, M.J. and Srisuk, N. 2017. Cyberlindnera tropicalis f.a., sp. nov., a novel yeast isolated from tropical regions. International Journal of Systematic and Evolutionary Microbiology, 67(8): 2569–2573.

Bonthong, P., Bunterngsook, B., Mhuantong, W., Aiewviriyasakul, K., Sritusnee, W., Champreda, V. and Lekakarn, H. 2025. Geno-mic and functional analysis of a novel yeast Cyberlindnera fabia-nii TBRC 4498 for high-yield xylitol production. Journal of Fungi, 11(6): 453.

Brysch-Herzberg, M., Dlauchy, D., Seidel, M. and Péter, G. 2021. Cyberlindnera sylvatica sp. nov., a yeast species isolated from forest habitats. International Journal of Systematic and Evolutionary Microbiology, 71(2): 004477.

Chai, C.Y., Jia, R.R., Chen, C.Y. and Hui, F.L. 2020. Blastobotrys baotianmanensis sp. nov. and Blastobotrys xishuangbannaensis f.a., sp. nov., two novel yeast species associated with insects and rotting wood. International Journal of Systematic and Evolu-tionary Microbiology, 70(7): 4217–23.

Chang, C.F., Huang, S.Y. and Lee, C.F., 2021. Vishniacozyma changhuana sp. nov., and Vishniacozyma taiwanica sp. nov., two novel yeast species isolated from mangrove forests in Taiwan. International Journal of Systematic and Evolutionary Micro-biology, 71(3): 004703.

Chang, C.F., Huang, L.Y., Chen, S.F. and Lee, C.F. 2012. Kloeckera taiwanica sp. nov., an ascomycetous apiculate yeast species isolated from mushroom fruiting bodies. International journal of systematic and evolutionary microbiology, 62(6): 1434–1437.

Crous, P.W., Cowan, D.A., Maggs-Kölling, G., Yilmaz, N., Larsson, E., Angelini, C., Brandrud, T.E., Dearnaley, J.D.W., Dima, B., Dovana, F. and Fechner, N. 2020. Fungal Planet description sheets: 1112–1181. Persoonia: Molecular Phylogeny and Evolu-tion of Fungi, 45: 251.

Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39(4): 783–791.

Fell, J.W. and Statzell, A.C. 1971. Sympodiomyces gen. n., a yeast-like organism from southern marine waters. Antonie Van Leeu-wenhoek, 37: 359–367.

Gungprakhon, P., Khammeankea, M., Limtong, S. and Khunnam-wong, P. 2025. Vishniacozyma siamensis sp. nov., a new anamor-phic tremellomycetous yeast species isolated from a mangrove forest in Thailand. International Journal of Systematic and Evolutionary Microbiology, 75(1): 006623.

Guamán-Burneo, M.C., Dussán, K.J., Cadete, R.M., Cheab, M.A., Portero, P., Carvajal-Barriga, E.J., da Silva, S.S. and Rosa, C.A. 2015. Xylitol production by yeasts isolated from rotting wood in the Galápagos Islands, Ecuador, and description of Cyberlin-dnera galapagoensis f.a., sp. nov. Antonie Van Leeuwenhoek, 108: 919–931.

Hoondee, P., Wattanagonniyom, T., Weeraphan, T., Tanasupawat, S. and Savarajara, A. 2019. Occurrence of oleaginous yeast from mangrove forest in Thailand. World Journal of Microbiology and Biotechnology, 35(7): 108.

Hsieh, C.W., Huang, L.Y., Tschen, E.F.T., Chang, C.F. and Lee, C.F. 2010. Five novel anamorphic, ascomycetous yeast species asso-ciated with mushrooms and soil. FEMS yeast research, 10(7): 948–956.

Into, P., Khunnamwong, P., Jindamoragot, S., Am-In, S., Intanoo, W. and Limtong, S. 2020. Yeast associated with rice phylloplane and their contribution to control of rice sheath blight disease. Micro-organisms, 8(3): 362.

Jackson, H.S. 1947. Trichomonascus, a new genus among simple Ascomycetes. Mycologia, 39: 709–715.

Kanpiengjai, A., Chui-Chai, N., Chaikaew, S. and Khanongnuch, C. 2016. Distribution of tannin-'tolerant yeasts isolated from Miang, a traditional fermented tea leaf (Camellia sinensis var. assamica) in northern Thailand. International Journal of Food Microbio-logy, 238: 121–131.

Khunnamwong, P., Jindamorakot, S. and Limtong, S. 2018. Endo-phytic yeast diversity in leaf tissue of rice, corn and sugarcane cultivated in Thailand assessed by a culture-dependent approach. Fungal Biology, 122(8): 785–799.

Khunnamwong, P., Kingphadung, K., Lomthong, T., Kanpiengjai, A., Khanongnuch, C. and Limtong, S. 2022. Wickerhamiella nakhonpathomensis f.a. sp. nov., a novel ascomycetous yeast species isolated from a mushroom and a flower in Thailand. International Journal of Systematic and Evolutionary Micro-biology, 72(1): 005191.

Khunnamwong, P., Nualthaisong, P., Kingphadung, K., Takashima, M., Sugita, T., Sumerta, I.N., Kanti, A., Kawasaki, H. and Lim-tong, S. 2025. Rhodotorula tropicalis sp. nov., a novel red yeast of the order Sporidiobolales isolated from Thailand, Indonesia and Japan. International Journal of Systematic and Evolutionary Microbiology, 75(3): 006701.

Kunthiphun, S., Chokreansukchai, P., Hondee, P., Tanasupawat, S. and Savarajara, A. 2018. Diversity and characterization of culti-vable oleaginous yeasts isolated from mangrove forests. World Journal of Microbiology and Biotechnology, 34(9): 125.

Khunnamwong, P., Nualthaisong, P., Sakolrak, B., Nutaratat, P. and Limtong, S. 2023. Yamadazyma sisaketensis f.a., sp. nov. and Yamadazyma koratensis f.a., sp. nov., two novel ascomycetous yeast species from mushrooms and cocoa leaves in Thailand, and reassignment of Candida andamanensis, Candida jaroonii and Candida songkhlaensis to the genus Yamadazyma. International Journal of Systematic and Evolutionary Microbiology, 73(11): 006174.

Kurtzman, C.P. 2010. Description of new yeast species-is one strain enough. Bull BISMiS, 1:17–24.

Kurtzman, C.P. 2011. Lindnera Kurtzman, Robnett & Basehoar & Powers (2008). In: Kurtzman CP, Fell JW, Boekhout T (eds). The Yeasts, a Taxonomic Study. 5th edn. Amsterdam, Elsevier, pp. 520–543.

Kurtzman, C.P. 2007. Blastobotrys americana sp. nov., Blastobotrys illinoisensis sp. nov., Blastobotrys malaysiensis sp. nov., Blasto-botrys muscicola sp. nov., Blastobotrys peoriensis sp. nov. and Blastobotrys raffinosifermentans sp. nov., novel anamorphic yeast species. International journal of systematic and evolutionary microbiology, 57(5): 1154–1162.

Kurtzman, C.P. and Robnett, C.J. 1998. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeu-wenhoek, 73: 331–371.

Kurtzman, C.P. and Robnett, C.J. 2007. Multigene phylogenetic analysis of the Trichomonascus, Wickerhamiella and Zygoascus yeast clades, and the proposal of Sugiyamaella gen. nov. and 14 new species combinations. FEMS Yeast Research, 7: 141–151.

Kurtzman, C.P., Fell, J.W., Boekhout, T. and Robert, V. 2011. Methods for isolation, phenotypic characterization and main-tenance of yeasts. In: Kurtzman CP, Fell JW, Boekhout T. (eds). The Yeasts, a Taxonomic Study. 5th edn. Amsterdam, Elsevier, pp. 87–110.

Kurtzman, C.P., Robnett, C.J. and Basehoar-Powers, E. 2008. Phylo-genetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov. FEMS Yeast Research 8: 939–954.

Lachance, M.A. 2012) In defense of yeast sexual life cycles: the forma asexualis–An informal proposal. Yeast Newsletter, 61: 24–25.

Limtong, S., Yongmanitchai, W., Tun, M.M., Kawasaki, H. and Seki, T. 2007. Kazachstania siamensis sp. nov., an ascomycetous yeast species from forest soil in Thailand. International Journal of Systematic and Evolutionary Microbiology, 57: 419–422.

Limtong, S., Youngmanitchai, W., Kawasaki, H. and Seki, T. 2008. Candida phangngensis sp. nov., an anamorphic yeast species in the Yarrowia clade, isolated from water in mangrove forests in Phang-Nga Province, Thailand. International journal of syste-matic and evolutionary microbiology, 58(2): 515–519.

Limtong, S. and Yongmanitchai, W. 2010. Candida chanthaburiensis sp. nov., Candida kungkrabaensis sp. nov. and Candida surat-ensis sp. nov., three novel yeast species from decaying plant materials submerged in water of mangrove forests. Antonie Van Leeuwenhoek, 98(3): 379–388.

Limtong, S., Kaewwichian, R., Yongmanitchai, W. and Kawasaki, H. 2014. Diversity of culturable yeasts in phylloplane of sugarcane in Thailand and their capability to produce indole-3-acetic acid. World Journal of Microbiology and Biotechnology, 30(6): 1785–1796.

Lindow, S.E. and Brandl, M.T. 2003. Microbiology of the phyllo-sphere. Applied and environmental microbiology, 69(4): 1875–1883.

Liu, S., Cai, D.Y. and Hui, F.L. 2024. Cyberlindnera qingyuanensis f.a., sp. nov., a yeast species isolated from rotting wood. Interna-tional Journal of Systematic and Evolutionary Microbiology, 74(8): 006507.

Liu, M., Jiang, Y.L., Zhang, Y.X. and Wang, Q.M. 2024a. Nakaza-waea tricholomae f.a, sp. nov., A novel ascomycetous yeast species isolated from two mushroom species in China. Current Microbiology, 81(3): 78.

Liu, M., Ding, R.X., Zhang, Y.X., Li, H.Z. and Wang, Q.M. 2024b. Wickerhamomyces corioli f.a., sp. nov., a novel yeast species discovered in two mushroom species. International Journal of Systematic and Evolutionary Microbiology, 74(4): 006333.

McNeill, J., Barrie, F.R., Buck, W.R., Demoulin, V., Greuter, W., Hawksworth, D.L., Herendeen, P.S., Knapp, S., Marhold, K., Prado, J. and Prudhomme Van Reine, W.F. 2012. International Code of Nomenclature for Algae, Fungi, and Plants (Melbourne Code) adopted by the Eighteenth International Botanical Congress Melbourne, Australia, July 2011 (Regnum Vegetabile 154). Koenigstein: Koeltz Scientific Books.

Minter, D.M. 2009. Cyberlindnera, a replacement name for Lindnera kurtzman, et al., nom. illegit. Mycotaxon. 110: 473–476.

Ninomiya, S., Mikata, K., Kajimura, H. and Kawasaki, H. 2013. Two novel ascomycetous yeast species, Wickerhamomyces scolyto-platypi sp. nov. and Cyberlindnera xylebori sp. nov., isolated from ambrosia beetle galleries. International Journal of Systematic and Evolutionary Microbiology, 63(7): 2706–2711.

Nouri, H., Moghimi, H., Geranpayeh Vaghei, M. and Nasr, S. 2018. Blastobotrys persicus sp. nov., an ascomycetous yeast species isolated from cave soil. Antonie van Leeuwenhoek, 111: 517–524.

Poomtien, J., Jindamorakot, S., Limtong, S., Pinphanichakarn, P. and Thaniyavarn, J. 2013. Two new anamorphic yeasts species, Cyberlindnera samutprakarnensis sp. nov. and Candida thasaen-ensis sp. nov., isolated from industrial wastes in Thailand. Antonie van Leeuwenhoek, 103(1): 229–238.

Santa-Brigída, R., Santos, A.R.O., Martins, M.B., Rosa, L.H., Lachance, M.A. and Rosa, C.A. 2024. Kodamaea schenbergiae f.a., sp. nov. and Suhomyces schwaniae f.a., sp. nov., two yeast species isolated from mushrooms and associated insects in a Brazilian Amazonian rainforest biome. International Journal of Systematic and Evolutionary Microbiology, 74(11): 006587.

Smith, M.T., de Hoog, G.S., Statzell‐Tallman, A. and Kurtzman, C.P. 2011a. Blastobotrys von Klopotek (1967). In: Kurtzman CP, Fell JW, Boekhout T (eds). The Yeasts, a Taxonomic Study. 5th edn. Amsterdam, Elsevier, pp. 959–979.

Smith, M.T., de Hoog, G.S., Malloch, D. and Kurtzman, C.P. 2011b. Trichomonascus HS Jackson emend. Kurtzman & Robnett (2007). In: Kurtzman CP, Fell JW, Boekhout T (eds). The Yeasts, a Taxonomic Study. 5th edn. Amsterdam, Elsevier, pp. 875–881.

Statzell-Tallman, A., Scorzetti, G. and Fell, J.W. 2010. Candida spencermartinsiae sp. nov., Candida taylorii sp. nov. and Pseu-dozyma abaconensis sp. nov., novel yeasts from mangrove and coral reef ecosystems. International journal of systematic and evolutionary microbiology, 60(8):1978–1984.

Tamura, K., Stecher, G. and Kumar, S. 2021. MEGA11: molecular evolutionary genetics analysis version 11. Molecular biology and evolution, 38: 3022–3027.

Thomas, S., Sanya, D.R., Fouchard, F., Nguyen, H.V., Kunze, G., Neuvéglise, C. and Crutz-Le Coq, A.M. 2019. Blastobotrys adeninivorans and B. raffinosifermentans, two sibling yeast species which accumulate lipids at elevated temperatures and from diverse sugars. Biotechnology for Biofuels, 12: 1–15.

van der Walt, J.P., Th Smith, M. and Yamada, Y. 1990. Arxula gen. nov. (Candidaceae), a new anamorphic, arthroconidial yeast genus. Antonie Van Leeuwenhoek, 57: 59–61.

Visagie, C.M., Boekhout, T., Theelen, B., Dijksterhuis, J., Yilmaz, N., Seifert, K.A. 2022. Da Vinci's yeast: Blastobotrys davincii f.a, sp. nov. Yeast, 40(1): 7–31.

Visagie, C.M., Meijer, M., Kraak, B., Groenewald, M., Houbraken, J., Theelen, B., Vorst, Y. and Boekhout, T. 2024. Blastobotrys nigripullensis, a new yeast species isolated from a fungal outbreak on an ancient Roman shipwreck in the Netherlands. Antonie van Leeuwenhoek, 117(1): 22.

Vu, D., Groenewald, M., Szöke, S., Cardinali, G., Eberhardt, U., Stielow, B., De Vries, M., Verkleij, G.J.M., Crous, P.W., Boek-hout, T.J.S.I.M. and Robert, V. 2016. DNA barcoding analysis of more than 9000 yeast isolates contributes to quantitative thres-holds for yeast species and genera delimitation. Studies in mycology, 85(1):91–105.

White, T.J., Bruns, T., Lee, S. and Taylor, J.W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (editor). PCR Protocols: A Guide to Methods and Applications. New York, Academic Press, pp. 315–322.

Zheng, J., Lu, Y.F., Liu, X.J. and Hui, F.L. 2017. Cyberlindnera xishuangbannaensis fa, sp. nov., a yeast isolated from rotting wood. International Journal of Systematic and Evolutionary Microbiology, 67(12): 5051–5055.

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Published

2025-10-20

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[1]
Khunnamwong, P., Gungprakhon, P. , Nualthaisong, P. , Sakolrak, B. , Srisuk, N. and Limtong, S. 2025. Three Novel Ascomycetous Yeast Species Isolated from Plant Leaves and Wild Mushrooms Collected in Thailand. Tropical Natural History. 25, 8 (Oct. 2025), 399–414. DOI:https://doi.org/10.58837/tnh.25.8.267392.

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No. 8 (2025): Supplement

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