Metschnikowia sirindhorniae f.a., sp. nov. and Moniliella princeps sp. nov., Two Novel Yeasts Species Isolated from Toddy Palm (Borassus flabellifer L.) Inflorescence Sap in Thailand
DOI:
https://doi.org/10.58837/tnh.25.8.267713Keywords:
Metschnikowia sirindhorniae f.s., sp. nov., Moniliella princeps sp. nov., novel yeast species, ThailandAbstract
Two strains, YSP-519 and YSP-478, respectively representing novel ascomycetous and basidiomycetous yeast species, were obtained from toddy palm inflorescence sap collected in Thailand. The ascomycetous yeast strain YSP-519 was closely related to Metschnikowia agaves CBS 7744T. There were 1.6% nucleotide substitutions (9 nucleotide substitutions and 2 gaps out of 555 nt) in the D1/D2 domains of the LSU rRNA gene and 0.7% nucleotide substitutions (3 nucleotide substitutions out of 454 nt) in the ITS regions. The basidiomycetous yeast strain YSP-478 was closely related to Moniliella pollinis CBS 461.67T. It had 0.5 % nucleotide substitutions (3 nucleotide substitutions out of 554 nt) in the D1/D2 domains of the LSU rRNA gene and 2.0% nucleotide substitutions (9 nucleotide substitutions out of 473 nt) in the ITS regions. Phylogenetic analysis based on the concatenated sequences of the ITS regions and the D1/D2 domains confirmed that the two strains represent two new species. The name Metschnikowia sirindhorniae f.a., sp. nov. is proposed for YSP-519T), and Moniliella princeps sp. nov. is proposed for YSP-478T. The holotypes of M. sirindhorniae and Mo. princeps are TBRC 10642T and TBRC 10641T, respectively and the ex-type are CGMCC 2.6079T and CGMCC 2.6078T, respectively. The MycoBank numbers of M. sirindhorniae and Mo. princeps are MB858878 and MB858879, respectively.
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