EFFICIENCY IMPROVEMENT OF BIOACTIVE COMPOUNDS EXTRACTION FROM CANTALOUPE AND MUSKMELON BY FREEZE-THAWING AND PEF
Keywords:antioxidant activity, cantaloupe, freeze-thawing, muskmelon, pulsed electric field
This study aimed to determine the effect of freeze-thawing cycle and pulsed electric field (PEF) pretreatment on extraction efficiency of bioactive compounds from cantaloupe and muskmelon peels. The peels were treated by either 0-5 cycles of freeze-thawing or 0-10,000 pulses of PEF at E = 143 V/cm, f = 100 Hz, and the pulse width of 50 µs prior to extraction by aqueous acetone or aqueous ethanol solvent. Antioxidant activity was determined by ferric reducing power (FRP) and DPPH methods. It was found that the acetone based extract of cantaloupe skin pretreated with 5 cycles of freeze-thawing gave the highest antioxidant activity (2,031.28±3.01 mg AAE/100 g and 400.39±93.94 mg AE/100 g for FRP and DPPH methods, respectively) with 2.96% extraction yield. On the other hand, the acetone based extract of cantaloupe skin pretreated with PEF for 10,000 pulses gave the antioxidant activity of 1,613.19 ± 37.61 mg AAE/100 g and 327.54±6.06 mg AAE/100 g for FRP and DPPH methods, respectively) with 2.37% extraction yield. Although the PEF treatment gave the extract with less antioxidant activity and less yield, the time used by this method was much shorter than that of freeze-thawing, i.e., 1 min 40 s for PEF and 5 days for freeze-thawing. Thus, PEF is considered to be more energy efficient and economically sound than freeze-thawing pretreatment. In addition, PEF pretreatment could yield greater extraction efficiency if higher electric field strength or number of pulses is applied.
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