EFFICIENCY IMPROVEMENT OF BIOACTIVE COMPOUNDS EXTRACTION FROM CANTALOUPE AND MUSKMELON BY FREEZE-THAWING AND PEF

  • Suvaluk Asavasanti KMUTT
  • Sukon Tantipaibulvut Department of Microbiology, Faculty of Science, King Mongkut’s University of Technology Thonburi, Bangkok, 10170
  • Anusaraporn Samaal Department of Microbiology, Faculty of Science, King Mongkut’s University of Technology Thonburi, Bangkok, 10170
  • Wiracha Sanuksaen Department of Microbiology, Faculty of Science, King Mongkut’s University of Technology Thonburi, Bangkok, 10170
Keywords: antioxidant activity, cantaloupe, freeze-thawing, muskmelon, pulsed electric field

Abstract

This study aimed to determine the effect of freeze-thawing cycle and pulsed electric field (PEF) pretreatment on extraction efficiency of bioactive compounds from cantaloupe    and muskmelon peels. The peels were treated by either 0-5 cycles of freeze-thawing or    0-10,000 pulses of PEF at E = 143 V/cm, f = 100 Hz, and the pulse width of 50 µs prior to extraction by aqueous acetone or aqueous ethanol solvent. Antioxidant activity was determined by ferric reducing power (FRP) and DPPH methods. It was found that the acetone based extract of cantaloupe skin pretreated with 5 cycles of freeze-thawing gave the highest antioxidant activity (2,031.28±3.01 mg AAE/100 g and 400.39±93.94 mg AE/100 g for FRP and DPPH methods, respectively) with 2.96% extraction yield. On the other hand, the acetone based extract of cantaloupe skin pretreated with PEF for 10,000 pulses gave the antioxidant activity of 1,613.19 ± 37.61 mg AAE/100 g and 327.54±6.06 mg AAE/100 g for FRP and DPPH methods, respectively) with 2.37% extraction yield. Although the PEF treatment gave the extract with less antioxidant activity and less yield, the time used by this method was much shorter than that of freeze-thawing, i.e., 1 min 40 s for PEF and 5 days for freeze-thawing. Thus, PEF is considered to be more energy efficient and economically sound than freeze-thawing  pretreatment. In addition, PEF pretreatment could yield greater extraction efficiency if higher electric field strength or number of pulses is applied.

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References

Asavasanti, S., Ristenpart, W., Stroeve, P. & Barrett, D.M. (2011). Permeabilization of plant tissues by monopolar pulsed electric fields: effect of frequency. Journal of Food Science. 76(1), E98-E111.

Aquino, C.F., Salomão, L.C.C., Ribeiro, S.M.R., De Siqueira, D.L. & Cecon, P.R. (2016). Carbohydrates, phenolic compounds and antioxidant activity in pulp and peel of 15 banana cultivars. Revista Brasileira de Fruticultura. 38(4), e-090.

Bobinaite, R., Pataro, G., Lamanauskas, N., Šatkauskas, S., Viškelis, P. & Ferrari, G. (2015). Application of pulsed electric field in the production of juice and extraction of bioactive compounds from blueberry fruits and their by-products. Journal of Food Science and Technology. 52(9), 5898–5905.

Bouras, M., Grimi, N., Bals, O. & Vorobiev, E. (2016). Impact of pulsed electric fields on olyphenols extraction from Norway spruce bark. Industrial Crops and Products. 80, 50–58.

Bodzon-Kulakowska, A., Bierczynska-Krzysik, A., Dylag, T., Drabik, A., Suder, P., Noga, M., Jarzebinska, J. & Silberring, J. (2007). Methods for samples preparation in proteomic research. Journal of Chromatography B. 849, 1–31.

Cook, R. (2009). Trends in the marketing of fresh produce and fresh-cut products. Retrieved November 17, 2017, from https://www.agecon.ucdavis.edu/people/faculty/facultydocs/Cook/Articles /freshcut2009Cook090922.pdf.

El-Din Ibrahim, M.E. & El-Masry, H.G. (2016). Phenolic content and antioxidant activity of antaloupe (Cucumis melo var. cantalupensis) and food application. International Journal of Nutrition and Food Sciences. 5(1), 16-24.

El Kantar, S., Boussetta, N., Lebovka, N., Foucart, F., Rajha, H.N., Maroun, R.G., Louka, N. & Vorobiev, E. (2017). Pulsed electric field treatment of citrus fruits : improvement of juice and polyphenols extraction. Innovative Food Science & Emerging Technologies. In press, Accepted Manuscript. https://doi.org/10.1016/j.ifset.2017.09.024.

Guo, C., Yang, J., Wei, J., Li, Y., Xu J. & Jiang, Y. (2003). Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Nutrition Research. 23(12), 1719-1726.

Hossain, M.B., Aguiló-Aguayoa, I., Lyng, J.G., Brunton, N.P. & Rai, D.K. (2015). Effect of pulsed electric field and pulsed light pre-treatment on the extraction of steroidal alkaloids from potato peels. Innovative Food Science and Emerging Technologies. 29, 9–14.

Ismail, H.I., Chan, K.W., Mariod, A.A. & Ismail, M. (2010). Phenolic content and antioxidant activity of cantaloupe (Cucumis melo) methanolic extracts. Food Chemistry. 119, 643–647.

James, J.B. & Ngarmsak, T. (2010). Processing of fresh-cut tropical fruits and vegetables : a technical guide. Retrieved November 17, 2017, from https://www.fao.org/docrep/014/i1909e/ i1909e00.pdf.

Johnson, B.H. & Hecht, M.H. (1994). Recombinant proteins can be isolated from E. coli cells by repeated cycles of freezing and thawing. Biotechnology. 12, 1357-1360.

Lester, G.E. (2008). Antioxidant, sugar, mineral, and phytonutrient concentrations across edible fruit tissues of orange-fleshed honeydew melon (Cucumis melo L.). Journal of Agricultural and Food Chemistry. 56(10), 3694–3698.

Li, Y., Guo, C., Yang, J., Wei, J., Xu, J. & Cheng, S. (2006). Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chemistry. 96(2), 254-260.

Luengo, E., Álvarez, I. & Raso, J. (2013). Improving the pressing extraction of polyphenols of orange peel by pulsed electric fields. Innovative Food Science and Emerging Technology. 17, 79–84.
Manzoor, M., Anwar, F., Bhatti, I.A. & Jamil, A. (2013). Variation of phenolics and antioxidant activity between peel and pulp parts of pear (Pyrus communis L.) fruit. Pakistan Journal of Botany. 45(5), 1521-1525.

Medina-Meza, I.G. & Barbosa-Cánovas, G.V. (2015). Assisted extraction of bioactive compounds from plum and grape peels by ultrasonics and pulsed electric fields. Journal of Food Engineering. 166, 268–275.

Menon, S.V. & Ramana Rao, T.V. (2012). Nutritional quality of muskmelon fruit as revealed by its biochemical properties during different rates of ripening. International Food Research Journal. 19(4), 1621-1628.

Metrouh-Amir, H., Duarte, C.M.M. & Maiza, F. (2015). Solvent effect on total phenolic contents, antioxidant, and antibacterial activities of Matricaria pubescens. Industrial Crops and Products. 67, 249-256.

Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology. 26(2), 211-219.

Parniakov, O., Barba, F.J., Grimi, N., Lebovka, N. & Vorobiev, E. (2016). Extraction assisted by pulsed electric energy as a potential tool for green and sustainable recovery of nutritionally valuable compounds from mango peels. Food Chemistry. 192, 842–848.

Sanino, L.B.S., Gonzaga, M.L.C., Soares, D.J., Lima, A.C.S., Lima, J.S.S., Almeida, M.M.B., Sousa, P.H.M. & Figueiredo, R.W. (2015). Bioactive compounds, antioxidant activity and minerals in flours prepared with tropical fruit peels. Acta Alimentaria. 44(4), 520–526.

Sasi Kumar R., Priyadharshini, S., Nandha Kumar K.P.L., Nivedha, S. (2014-15). In vitro pharmacognostical studies and evaluation of bioactive constituents from the fruits of cucumis melo L. (Muskmelon). International Journal of Pharmacognosy and Phytochemical Research. 6(4); 936-941.

Singh, J., Singh, V., Shukla, S., & Rai, A.K. (2016). Phenolic content and antioxidant capacity of selected cucurbit fruits extracted with different solvents. Journal of Nutrition & Food Sciences. 6(565). DOI: 10.4172/2155-9600.1000565.

Singhal, M., Paul, A., Singh, H.P., Dubey, S.K. & Gaur, K. (2011). Evaluation of reducing power assay of chalcone semicarbazones. Journal of Chemical and Pharmaceutical Research. 3(3), 639-645.

Vorobiev, E., & Lebovka, N. (2010). Enhanced extraction from solid foods and biosuspensions by pulsed electrical energy. Food Engineering Reviews. 2(2), 95-108.

Xue, Z., Feng, W., Cao, J., Cao, D. & Jiang, W. (2009). Antioxidant activity and total phenolic contents in peel and pulp of Chinese jujube (Ziziphus jujube Mill.) fruits. Journal of Food Biochemistry. 33(5), 613–629.
Published
2018-03-08
Section
บทความวิจัย (Research Article)