UTILIZATION OF STARCH HYDROLYZED WASTE FROM SUGAR FACTORY FOR PRODUCING YEAST BIOMASS: OPTIMUM CONDITION AND KINETICS
Keywords:Starch hydrolyzed waste, Yeast biomass, Saccharomyces cerevisiae, Aeration rate, Mathematical modeling
The aim of this research was to study the utilization of starch hydrolyzed waste (SHW) from glucose manufacturing as a raw material for producing yeast biomass Saccharomyces cerevisiae. Moreover, aeration rate of the yeast cultivation in fermenter scale was investigated. The result showed that total soluble solids, pH, and yeast extract affected to biomass production from SHW significantly. In addition, the quadratic equation for predicting the optimum condition was Biomass (g/L) = 2.38 + 0.36A + 1.13C - 0.021D + 0.052AC - 0.10AD - 0.042CD – 0.18A2 - 0.76C2 - 0.28D2. The optimum condition was total soluble solids, pH, and yeast extract for 16.73 °Brix, 4.84, and 8.78 g/L, respectively. Under this condition, the highest biomass was obtained for 2.95 g/L of shaking flask scale. Biomass production in 5-L fermenter demonstrated that the aeration rate influenced the biomass production. The maximum biomass and maximum biomass production rate at 1.0 vvm were higher than at 0.5 vvm for 10% and 75% approximately, respectively. Furthermore, experimental result denoted that the Logistic model and modified Gompertz model could simulate the growth of yeast in SHW as well.
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