OPTIMIZATION OF MEDIA AND SUCROSE ADDITION TIME FOR DEXTRAN PRODUCTION USING LACTIC ACID BACTERIA Leuconostoc mesenteroides TISTR053

Authors

  • Theppanya Charoenrat 1Department of Biotechnology, Faculty of Science and Technology, Thammasat University

Keywords:

Dextran, Lactic Acid Bacteria, Leuconostoc mesenteroides TISTR053, Cultivation medium, Sucrose addition time

Abstract

Dextran is an exopolysaccharide (EPS) from lactic acid bacteria (LAB) Leuconostoc mesenteriodes. It is mostly produced by fermentation process and has been widely applied in the food and medical industries. In addition to the development of production process that has influenced on yield and dextran content, other factors affecting to enhance microbial cultivation must be considered to achieve high dextran productivity. The objectives of this research were therefore to study the type of culture media (De Man Rogosa and Sharpe; MRS, glucose synthetic medium and sucrose synthetic medium) on cell growth and sucrose addition time during fermentation to obtain the highest yield. According to the parameters related to growth, the results showed that the suitable medium for growth was sucrose synthesis medium with lag time for 1.5 h. It provided viable cell concentration of (6.03 ± 0.39) x 1010 cfu/mL which higher than glucose synthetic medium and MRS around 1.3 and 1.4 times, respectively. The influence of sucrose addition time on dextran production revealed that sucrose added at 2 h of fermentation was optimum with dextran content and volumetric productivity of 29.63 ± 0.42 g/L and 2.69 ± 0.08 g/L∙h, respectively. These results were higher than when sucrose was added at 0, 4, 6, and 8 about 1.2, 1.7, 2.4, and 3 times, respectively. Regarding the results, further study should be developed on dextran production in bioreactor scale.

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References

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Published

2019-07-19

Issue

Section

บทความวิจัย (Research Article)