• Wisarat Piya
  • Anitthan Srinual


Leaf anatomy, Leaf epidermis, Diospyros, Ebenaceae, Thailand


Leaf epidermal studies of 22 species of the genus Diospyros L. in Thailand were investigated. The leaf specimens were studied by using peeling and clearing methods, stained with 1% safranin in 70% ethanal. The results revealed general anatomical characteristics of this genus including: 1) three types of cuticular ornamentation which are smooth, straight or anonymous branching 2) the shapes of epidermal cell which included jigsaw-like, polygonal or irregular shapes 3) the patterns of cell walls are striated, cleft or emarginate form 4) the types of stomata are anomocytic, actinocytic, or cyclocytic 5) the types of trichome are unicellular hair, glandular hair and T-shape unicellular hair 6) the types of inclusions are prismatic crystal, dark red or dark brown staining. Some species further showed the secretory cavity in epidermis and inclusions in guard cells. These mentioned anatomical characteristics could be used to construct a dichotomous key for species identification in this genus.



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บทความวิจัย (Research Article)