GENETIC DIVERSITY OF PARAH TREE (Elateriospermum tapos) IN NOPPHITUM DISTRICT, NAKHON SI THAMARAT PROVINCE

  • ๋Juthamas Suppapan Faculty of Education, Nakhon Si Thammarat Rajabhat University
  • Verakiat Supmee Faculty of Science and Technology, Rajamangala University of technology Srivijaya
  • Phatthanaporn Rinthajak Khaonan Nationnal Park
Keywords: parah tree, genetic diversity, Nakhon Si Thammarat

Abstract

The parah tree (Elateriospermum tapos) is a species of forest and tree found in the Noppitum district, Nakhon Si Thammarat Province. At present, the number of trees has decreased due to deforestation. Therefore, genetic information should be available for use in management planning. The objectives of this research were (1) to analyze the genetic diversity of the parah trees, (2) to analyze the population genetic structure of the parah trees, and (3) to analyze the demographic history of the parah trees in the Noppitum district, Nakhon Si Thammarat Province. Leave samples were collected from four localities in the Noppitum district (natural forest: Huay lek, Hin Tor Waterfall, planted forest: Hin Tor Waterfall, Sunantha Waterfall). DNA was extracted and nucleotide sequences were analyzed from gene ribulose-bisphosphate carboxylase (rbcL) in chloroplast DNA. To analyze data, nucleotide sequences of parah trees from Malaysia and Brunei in National Center for Biotechnology Information (GenBank) were used for analysis. The total nucleotide sequence was 121 sequences. The result showed 3 haplotypes. The haplotype diversity was in the range of 0.000-0.667 and the nucleotide diversity was in the range of 0.00000-0.00103. The result of the study of population genetic structure shows that dividing the population into two groups (Noppitum group and MalayBrunei group). Results of the demographic history study found that the parah tree population in the Noppitum district has not expanded the population size.

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References

Avise, J.C., Neigel, J.E., & Arnold, J. (1984). Demographic influences on mitochondrial DNA lineage

survivorship in animal populations. Journal of Molecular Evolution, 20, 99-105.

Ayala, F.J. (1982). Population and Evolutionary Genetics: A Primer. The Benjamin Cummings Pub.

Co. Inc, California.

Baimai, V., & Tanthalekha, R. (2007). Khaonan-Pamek: Natural and Global Warming. Bangkok:

Bangkok Ltd. (in Thai)

Deng, Y., Liu, T., Xie, Y., Wei, Y., Xie, Z., Shi, Y., & Deng, X. (2020). High genetic diversity and low

differentiation in Michelia shiluensis, an endangered magnolia species in South China. Forests, 11, 469; DOI: 10.3390/f11040469.

Excoffier, L., & Lischer, H.E.L. (2010). Arlequin suite ver 3.5: A new series of programs to perform

population genetics analyses under Linux and Windows. Molecular Ecology Resources, 10, 564-567.

Feng, X., Wang, Y., & Gong, X. (2014). Genetic diversity, genetic structure and demographic history

of Cycas simplicipinna (Cycadaceae) assessed by DNA sequences and SSR markers.

BMC Plant Biology, 14,187.

Fu, F.X. (1997). Statistical tests of neutrality of mutations against population growth, hitchhiking

and background selection. Genetics, 147, 915–925.

Gao, Y., Yin, S., Wu, L., Dai, D., Wang, H., Liu, C., & Tang, L. (2017). Genetic diversity and structure

of wild and cultivated Amorphophallus paeoniifolius populations in southwestern China

as revealed by RAD-seq. Scientific Reports 7, 14183.

Harpending, R.C. (1994). Signature of ancient population growth in a low-resolution mitochondrial

DNA mismatch distribution. Human Biology, 66, 591-600.

Huang, J.C., Wang, W.K., Peng, C.I., & Chiang T. Y. (2005). Phylogeography and conservation genetics

of Hygrophila pogonocalyx (Acanthaceae) based on atpB-rbcL noncoding spacer cpDNA. Journal of plant research, 118, 1-11.

Jaroensutasinee, M. (2008). The impact of climatic factors on the appearance of parah tree

Retrieved from http://www.thaienergynews.com (in Thai), [2019, 20 September.]

Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin,

F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J., & Higgins, D.G. (2007).

Sequence analysis Clustal W and Clustal X version 2.0. Bioinformatics Applications Note,(21), 2947-2948.

Librado, P., & Rozas, J. (2009). DnaSP v5: a software for comprehensive analysis of DNA polymorphism

data. Bioinformatics, 25, 1451-1452.

Nei, M. (1987). Molecular Evolutionary Genetics. New York: Columbia University Press. Ramirez-Soriano A., Ramos-Onsins, S.E., Rozas, J.F., & Navarro, A. (2008). Statistical power analysis of neutrality tests under demographic expansions, contractions and bottlenecks with recombination. Genetics, 179, 555-567.

Rogers, A.R., & Harpending, H. (1992). Population growth makes waves in the distribution of pairwise

genetic differences. Molecular Biology and Evolution, 9, 552-569.

Slatkin, M. (1987). Rare alleles as indicators of gene flow. Evolution, 39(1), 53-65.

Su, Y.J., Wang, T., Zheng, B., Jiang, Y., Ouyang, P.Y., & Chen, G.P. (2005). Genetic variation and phylogeographical patterns in Alsophila podophylla from Southern China based on cpDNA

atpB-rbcL sequence data. American Fern Journal, 95(2), 68-79.

Sun, Y., Yang, H., Zhang, Q., Qin, L., Li, P., Lee, J., Chen, S., Rahman, K., Kang, T., & Jia, M. (2019).

Genetic diversity and its conservation implications of Vitex rotundifolia (Lamiaceae) populations in East Asia. Retrieved from http://doi.org/10.7717/peerj.6194

Tajima, F. (1989). Statistical method for testing the neutral mutation hypothesis by DNA polymorphism.

Genetics, 123, 585–595.

Tamura, K., Dudley, J., Nei, M., & Kumar, S. (2007). MEGA 4: molecular evolutionary genetics analysis

(MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599.

Tokuoka, T. (2007). Molecular phylogenetic analysis of (Euphorbiaceae sensu stricto) based on plastid

and nuclear DNA sequences and ovule and seed character evolution. Journal of plant

research, 120(4), 511-522.

Wurdack, K.J., Hoffmann, P., & Chase, M.W. (2005). Molecular phylogenetic analysis of uniovulate

Euphorbiaceae (Euphorbiaceae sensu stricto) using plastid RBCL and TRNL-F DNA sequences.

American journal of botany, 92(8), 1397-1420.

Yang, Z. (2006). Computational molecular evolution. Oxford University Press, New York.

Zhao, Y.J., & Gong, X. (2012). Genetic structure of the endangered Leucomeris decora (Asteraceae)

in China inferred from chloroplast and nuclear DNA markers. Conservation genetics, 13, 271-281

Published
2021-06-02
Section
บทความวิจัย (Research Article)