• Thanaphorn Rakkan Faculty of Science, Thaksin University
  • Kanokphorn Sangkharak Faculty of Science, Thaksin University


Biodiese, Bioethanol, Glycerol, Polyhydroxyalkanoates, Polyhydroxybutyrate


This study aimed to produce biodiesel from used vegetable oil. Moreover, glycerol (the by-product) from biodiesel process was also utilized for bioethanol and polyhydroxyalkanoate production. The experiment was divided into 2 steps. First, the production of biodiesel from used vegetable oil and methanol by transesterification reaction using potassium hydroxide as catalyst. The condition for biodiesel production containing 6:1 oil to methanol ratio, 1% catalyst, 250 rpm at 60 ºC for 1 h. Under these condition, 90.92±0.06% of biodiesel with 96.5±0.01% methyl ester was obtained. In addition, the acid value, free fatty acid, saponification value, molecular weight (Potentiometric Titration), pour point and cloud point of biodiesel were 0.45±0.02 mg KOH/g oil, 0.21±0.02%, 153.3±0.15 352±0.15 g/mol, 18 ºC and 16 ºC, respectively. The characteristic of biodiesel was met the standard of Department of Energy Business (Thailand). Afterward, the glycerol by-product was isolated from biodiesel and utilized as carbon source for bioethanol and polyhydroxyalkanoate production using C. necator TISTR 1095 and S. cerevisiae under batch fermentation (150 rpm, 37 ºC). The result found that the NB supplemented with 10% glycerol yield highest bioethanol at 1.94% after 48 h cultivation. While, the highest polyhydroxyalkanoate (3.98 g/L) obtained from NB supplemented with 10% glycerol and 4% palm fiber after 84 h cultivation


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Banerjee, N., Ramakrishnan, R., & Jash, T. (2014). Biodiesel production from used vegetable oil collected

from shops. Energy Procedia, 54, 161-165.

Bhandasak, T., Ponchumni, P., Tanikku, P., Sawasdee, V., & Sawasdee, N. (2018). Polyhydroxyalkanoates

production from fatty acids of palm oil using Pseudomonas fluorescens TISTR 358. The Journal of Industrial Technology, 14(3), 1-14.

Diasakou, M. (1998). Kinetics of the non-catalytic transesterification of soybean oil. Fuel, 77, 1297-1302.

Kawentar, W.A., & Budiman, A. (2013). Synthesis of biodiesel from second-used cooking oil. Energy Procedia, 32, 190-199.

Kumneadklang, S., Arhma, A, Sangkarak, K., & O-Thong, S. (2012). Ethanol production from chemical

pretreated oil palm trunk by yeast Saccharomyces cerevisiae. Thaksin University Journal, 15(3), 116-123.

Luque, R., Lovett, J.C., Clancy, J., Campelo, J.M., & Romero, A.A. (2010). Biodiesel as feasible petrol

fuel replacement: a multidisciplinary overview. Energy & Environmental Science, 3(11), 1706-1721.

Miller, G.L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical

Chemistry, 31, 717-724.

Niwaswong, C., & Ruangviriyachai, C.(2012). Production of cellulosic ethanol in thailand. KKU Science

Journal, 40(4), 1073-1088.

Padermshoke, A., Katsumoto, Y., Sato, H., Ekgasit, S., & Noda, I.(2004). Surface melting and crystallization

behavior of polyhydroxyalkanoates studied by attenuated total reflection infrared spectroscopy. Polymer, 45, 6547-6554.

Phol-in, S. (2011). Screening and production of poly-beta-hydroxybutyrate (PHB) by marine

Microorganism. Master Thesis, Department of Biotechnology Silpakorn University. (in Thai)

Rafati, A., Tahvildari, K., & Nozari, M. (2018). Production of biodiesel by electrolysis method from

waste cooking oil using heterogeneous MgO-NaOH nano catalyst. Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 1-14.

Rakkan, T., Suwanno, S., Paichid, N., Yunu, T., Klomklao, S., & Sangkharak, K.(2017).Optimized synthesis

method for transesterification of residual oil from palm oil mill effluent and lipase from pacific white shrimp (Litopenaeus Vannamei hepatopancreas to environmentally friendly biodiesel. Fuel, 209, 309-314.

Ramezani, M., Amoozegar, M.A., & Ventosa, A. (2015). Screening and comparative assay of polyhydroxyalkanoates produced by bacteria isolated from the Gavkhooni Wetland in Iran and evaluation of poly-β-hydroxybutyrate production by halotolerant bacterium Oceanimonassp. GK1. Annals of Microbiology, 65(1), 517–526.

Sonnam, S., Yunu, T., Paichid, N., & Sangkharak, K. (2015). Optimization of polyhydroxybutyrate production by Alcaligenes eutrophus and its application. Thaksin University Journal, 18(1), 49-56.

Suksawat, S., & Pathom-aree, W. (2012). The role of bacteria on bioplastic. Srinakhrinwirot Science

Journal, 28(2). (in Thai).

Suwanno, S., Rakkan, T., Yunu, T., Paichid, N., Kimtun, P., Prasertsan, P., & Sangkharak, K. (2017). The

production of biodiesel using residual oil from palm oil mill effluent and crude lipase from oil palm fruit as an alternative substrate and catalyst. Fuel, 195, 82-87.





บทความวิจัย (Research Article)