IDENTIFICATION OF YEASTS ISOLATED FROM KHAOPLOUNG FOREST BY COMPARTIVE ITS AND D1/D2 SEQUENCE ANALYSIS
Keywords:
Local yeast, Khaoploung forest, ITS, D1/D2 domainAbstract
The objective of this research was to identify the yeast species found in the Khaoploung forest area. The results showed that the 126 isolated yeasts were grown on acidified yeast extract malt extract agar which contained the antibiotic chloramphenicol at the concentration of 200 mg / l and 0.1 % propionic acid (pH 3.7). In a comparative study of the nucleotide sequences of ITS, 3 yeast species were identified; namely, Candida tropicalis PRUCT1, Trichosporon asahii PRUTA1, and Pichia sp. PRUPS1, with similarity percentage at 100, 100 and 99.12, respectively, and the nucleotide sequence of the D1/D2 domain of large subunit ribosomal DNA (LSU rDNA) or 26S rDNA was found in Dipodascus albidus PRUDA1 with a percentage of similarity at 91.47
References
Alfred B. (2011). The importance and ecology of yeasts in soil, Soil Biology and Biochemistry, 43(1), 1-8. Retrieved from https://doi.org/10.1016/j.soilbio.2010.10.001. [2021, 23 Jun.]
Bamrungsawad, K. (2012). Development of A Nature Trail in the Khaoploung Forest at Chaibadanpiphat College, Phranakhon Rajabhat University, Amphur Chai Badan, Lop Buri Province. (Master's thesis). Phranakhon Rajabhat University, Bangkok. (in Thai)
Barnett, J.A., Payne, R.W. & Yarrow, D. (2000). Yeasts: Characteristics and Identification, 3rd edition. Cambridge University, New York.
Boonmak, C., Jindamorakot, S., Kawasaki, H., Yongmanitchai, W., Suwanarit, P., Nakase, T. & Limtong, S. (2009). Candida siamensis sp. nov., an anamorphic yeast species in the Saturnispora clade isolated in Thailand. FEMS: Yeast Research, 9(4), 668-672.
Chanklan, R., Kungkaew, P., Am-In, S. & Jindamorakot, S. (2012) Diversity of yeasts in the nature education center for mangrove conservation and ecotourism, Chonburi Province. Thai Journal of Science and Technology,1(3), 155-168.
Chongsuwat, W. (2011) Study Report No.3 Survey Design and Master Plan Project Chai Badan Phiphat College Phranakhon Rajabhat University. Phranakhon Rajabhat University, Bangkok. (in Thai)
Fell, J.W., Boekhout, T., Fonesca, A., Scorzetti, G. & Tallman, A.S. (2000). Biodiversity and systematics of basdiomycetous yeasts determined by large subunit rDNA D1/D2 domain sequence analysis. International Journal of Systematic and Evolutionary Microbiology, 50, 1351-1371.
Haelewaters, D., Toome-Heller, M., Albu, S., & Aime, M. C. (2020). Red yeasts from leaf surfaces and other habitats: three new species and a new combination of Symmetrospora (Pucciniomycotina, Cystobasidiomycetes). Fungal systematics and evolution, 5, 187-196. Retrieved from https://doi.org/10.3114/fuse.2020.05.12 [2021, 23 Jun.]
Hermansyah, N. & Wiraningsih, M. (2016). Bioethanol production from cellulose by Candida tropicalis, as an alternative microbial agent to produce ethanol from lignocellulosic biomass. Sriwijaya. Journal of Environment, 1(1), 10-13.
Jamai, L. & Ettayebi, M. (2013). Bioethanol production process using the non-conventional yeast Candida tropicalis. International Renewable and Sustainable Energy Conference (IRSEC), 477-481.
Kadhim, R.A., Al-Saadoon, A.H., & Al-Mahmoud, W.A. (2019). Morphological and phylogenetic identification of Pichia species associated with foods in Basrah, Iraq. Basrah Journal of Science, 37(2), 223-236.
Kaewwichian, R. & Khamthaiklang, S. (2017). Yeast in Mangrove Forest Soil from the Central Thailand and Its Ability in the Degradation of Starch, Carboxymethyl cellulose and Xylan. Burapha Science Journal, 22(3). (in Thai)
Kuncharoen, N., Techo, S., Savarajara, A. & Tanasupawat, S. (2020). Identification and lipolytic activity of yeasts isolated from foods and wastes. Mycology, 11(4), 279-286. Retrieved from DOI: 10.1080/21501203.2020.1745922 [2021, 23 Jun.]
Kurtzman, C.P., Fell, J.W. & Boekhout T. (2011). The Yeasts (Fifth Edition). Elsevier Science. Inc., Amsterdam.
Kurtzman, C.P. & Robnett, C.J. (1998). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences, Antonie van Leeuwenhoek, 73, 331-371.
Kurtzman, C.P. & Sugiyama, J. (2001). Ascomycetous yeasts and yeastlike taxa, In D.J. McLaughlin and P.A. Lemke, eds. The Mycota VII: Systematics and Evolution Part A. Springer-Verlag, Berlin. 179-200.
Lagerheim, G. (1892). Dipodascus albidus, eine neue, geschlechtliche Hemiascee. Jahrb. Wiss. Bot., 24, 549-565.
Li A.H., Yuan F.X., Groenewald M., Bensch K., Yurkov A.M., Li K., Han P.J., Guo L.D., Aime M.C., Sampaio J.P., Jindamorakot S., Turchetti B., Inacio J., Fungsin B., Wang Q.M., & Bai F.Y. (2020). Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species. Studies in Mycology, 96, 17-140. Retrieved from https://doi.org/10.1016/j.simyco. 2020.01.002. [2021, 23 Jun.]
Limtong, S., Sintara, S., Suwannarit, P., & Lotong, N. (2002). Yeast diversity in Thai traditional fermentation starter (Loog-pang). Kasetsart Journal (Natural Sciences) (Thailand), 36(2), 149-158.
Limtong, S., Srisuk, N., Yongmanitchai, W., Kawasaki, H., Yurimoto, H., Nakase, T. & Kato, N. (2004). Three new thermotolerant methylotrophic yeasts. Candida krabiensis sp. nov., Candida sithepensis sp. nov., and Pichia siamensis sp. nov., isolated in Thailand. Journal of General and Applied Microbiology, 50, 119-127.
Limtong, S., Yongmanitchai, W., Kawasaki, H. & Seki, T. (2007). Candida thaimueangensis sp. nov., an anamorphic yeast species from estuarine water in a mangrove forest in Thailand. International Journal of Systematic and Evolutionary Microbiology, 57, 650-653.
Limtong, S., Youngmanitchai, W., Kawasaki, H. & Seki, T. (2008) Candida phangngensis sp. nov., an anamorphic yeast species in the Yarrowia clade, isolated from water in mangrove forests in Phang-Nga Province, Thailand. International Journal of Systematic and Evolutionary Microbiology, 58, 515-519.
Limtong, S. & Yongmanitchai, W. (2010). Candida chanthaburiensis sp. nov., Candida kungkrabaensis sp. nov. and Candida suratensis sp. nov., three novel yeast species from decaying plant materials submerged in water of mangrove forests. Antonie van Leeuwenhoek, 98, 379-388.
Limtong, S. (2013). Yeast genetics and guidelines for utilization. Thai Journal of Genetics, S.1(S1 (Special Issue 1)), 71-75. (in Thai)
Lopandic, K. and Prillinger, H. (2001). Genotypic identification and phylogenetic characteriza-tion of yeasts from Austrian dairy products. Fachgruppe Mikrobiologice und Molekularbiologir. 117-118.
Oslan S.N., Salleh A.B., Rahman R.N., Basri M. & Chor A.L.T. 2012. Locally isolated yeasts from Malaysia: identification, phylogenetic study and characterization. Acta Biochimica Polonica 59(2). 225–229. Retrieved from www.actabp.pl [2021, 23 Jun.]
Passoth V., Fredlund E., ÄdelDruvefors U., & Schnürer J. (2005). Biotechnology, physiology and genetics of the yeast Pichia anomala. Yeast Research. 1567-1364
Prasatsri, K. (2006). Identification of Yeast isolated from organic matter in mangrove forest by conventional and molecular taxonomy. Master of Science (Microbiology). Kasetsart University. (in Thai)
Rungcharoen, C., Phunan, W., Nilsuwan, A., Boonme, N., Keawprasert, K. & Somwong-in. (2019). Summary report on the performance of the resource protection project in the Khao Phluang forest area Activities to explore biological resources in the mushroom group that are useful in the ecosystem in the Khao Phluang forest area Chai Badan Phiphat College Lopburi. (Academic reports). Phranakhon Rajabhat University, Bangkok. (in Thai)
Sasiapa Boonkong. (2021). Development of yogurt with Nypa fruitcans in syrup. Phranakhon Rajabhat Research Journal (Science and Technology), 16(2), 1-11. (in Thai)
Siringam, T., Vanijajiva, O. and Thaweesuk, A. (2019). Resource Protection Project in the Khao Phluang Forest Area: Survey and Plant Coding, and Coordinating Code Activity at Chaibadanpiphat College, Lopburi Province. (Academic Report). Phranakhon Rajabhat University, Bangkok. (in Thai)
Soetarto, E.S. (1998). Xylan degrading enzymes of the yeast Candida tropicalis strain R1. Indonesian Journal of Biotechnology. 173-179.
Solairaj, D., Legrand, N.N.G., Yang, Q., & Zhang, H. (2020). Isolation of pathogenic fungi causing postharvest decay in table grapes and in vivo biocontrol activity of selected yeasts against them. Physiological and Molecular Plant Pathology, 110. Retrieved from https://doi.org/10.1016/j.pmpp.2020.101478. [2021, 23 Jun.]
Sulman, S. & Rehman, A. (2013). Isolation and Characterization of Cellulose Degrading Candida tropicalis W2 from Environmental Samples. Pakistan Journal of Zoology, 45(3), 809-816.
Sumpradit, T. (2005). Yeast diversity in soils from hill evergreen, mixed deciduous, dry dipterocarp, and pine forests of Nam Nao National Park. Kasetsart University/Bangkok. Retrieved from DOI: https://doi.nrct.go.th/ ListDoi/listDetail? Resolve_DOI= [2021, 23 Jun.]
Sumpradit, T., Wongvilair, R., & Wiangdao, S. (2015). Yeast Species Diversity in Soils Collected from the Mixed Deciduous and Dry Evergreen Forests in Mae Wong National Park. (Master's thesis). Naresuan University, Phitsanulok. (in Thai)
Tapia-Vázquez, I., Sánchez-Cruz, R., Arroyo-Domínguez, M., Lira-Ruan, V., Sánchez-Reyes, A., Sánchez-Carbente, M.R., Padilla-Chacón, D., Batista-García, R.A., & Folch-Mallol, J.L. (2020). Isolation and characterization of psychrophilic and psychrotolerant plant-growth promoting microorganisms from a high-altitude volcano crater in Mexico. Microbiological Research, 232. Retrieved from https://doi.org/ 10.1016/j.micres.2019.126394. [2021, 23 Jun.]
Thongekkaew, J., Khumsap, A. & Chatsa-nga, P. (2011). Yeasts in mixed deciduous forest areas of Phujong Nayoy National Park and their ability to produce xylanase and carboxymethyl cellulase. Songklanakarin. Journal of Science and Technology, 34 (2), 157-163.
Vandamme P., Pot B., Gillis M., P de Vos, Kersters K., & Swings, J. (1996). Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiological Reviews, 60(2), 407-438.
White, T.J., Bruns, T., Lee, S. and Taylor, J.W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M, Gelfand D, Sninsky JJ & White TJ (Eds) PCR protocols Academic Press, San Diego, California. 315–322.
Yurkov A. M. (2018). Yeasts of the soil - obscure but precious. Yeast (Chichester, England), 35(5), 369–378. Retrieved from https://doi.org/10.1002/yea.3310. [2021, 23 Jun.]
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
โปรดกรอกเอกสารและลงนาม "หนังสือรับรองให้ตีพิมพ์บทความในวารสารวิจัยมหาวิทยาลัยราชภัฏพระนคร สาขาวิทยาศาสตร์และเทคโนโลยี" ก่อนการตีพิมพ์
