Roles of Mangosteen, Durian Products and Their Combinations in Reduction of Mutagenesis and Mutagen Formation: Studies on Nitrite Treated 1-Aminopyrene

Abstract

Mangosteen, durian products and their different combinations were investigated for their mutagenicity, antimutagenicity and inhibition on mutagen formation by Ames test. All tested samples (dimethylsulfoxide extracts) showed no mutagenic potential towards Salmonella typhimurium strains TA98 and TA100 without metabolic activation after nitration at pH 3.0-3.4. Each sample was further evaluated for its mutagenicity against the products from 4-h nitration of 1-aminopyrene (AP-nitrite model). It was demonstrated the possibility of the samples in reducing direct-acting mutagenicity of compounds occurred in the model on both strains. Each tested sample was also added into the model at the beginning of the reaction to observe its inhibitory effect on mutagen formation. The results revealed the potentials of the samples in inhibiting mutagen formation. In conclusion, the reduction of the direct-acting mutagenenesis and the inhibition on mutagen formation of the DMSO extract of mangosteen, durian products and their combinations were detected in both S. typhimurium strains TA98 and TA100.